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Species: A. niger
Genetic Insert: pTEF1-cexA-tCYC1
Vector Backbone Description: Backbone Marker:Matthias Steiger; Vector Backbone:MST606; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30553933
Proper citation: RRID:Addgene_118076 Copy
Vector Backbone Description: Vector Backbone:pBSV2H; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/21/363390 for bioRxiv preprint.
Addgene QC identified a partial duplication of the segment of the plasmid containing the HygR selectable marker. The plasmid is functional for transformation and selection in B. burgdorferi. This plasmid was functional for use as described in Takacs et al. 2018.
Proper citation: RRID:Addgene_118229 Copy
Vector Backbone Description: Backbone Size:5835; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126598 Copy
Species: Synthetic
Genetic Insert: sgRNAflaB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Comments: Addgene QC identified an IS element present in the backbone sequence. The depositing lab does not believe this impacts the functional use of the plasmid but requesting scientists should validate expected behavior prior to experimentation.
Proper citation: RRID:Addgene_149561 Copy
Species: Synthetic
Genetic Insert: sgRNAmreB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149567 Copy
Species: Synthetic
Genetic Insert: sgRNArodA
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149569 Copy
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9H(-10TC); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149587 Copy
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pJDC89; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:26934495
Proper citation: RRID:Addgene_157876 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cyclin D3;1
Vector Backbone Description: Backbone Marker:15572; Vector Backbone:pGWB2; Vector Types:Plant Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31679162
Proper citation: RRID:Addgene_140750 Copy
Species: Synthetic
Genetic Insert: crRNA to ligD
Vector Backbone Description: Vector Backbone:pMN016; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30039929
Comments: Shuttle vector Mycobacterium smegmatis/E.coli
Proper citation: RRID:Addgene_104623 Copy
Species: Other
Genetic Insert: HH - FS23 linker - HDV
Vector Backbone Description: Vector Backbone:Episomal ARS/CEN Backbone; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30737742
Comments: Additional Publication: Prielhofer, R. etal (2017) BMC Syst Biol. 11,123 (PMID:29221460)
Proper citation: RRID:Addgene_104907 Copy
Species: Other
Genetic Insert: HH - FS23 linker - HDV
Vector Backbone Description: Vector Backbone:Episomal ARS/CEN Backbone; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30737742
Comments: Additional Publication: Prielhofer, R. etal (2017) BMC Syst Biol. 11,123 (PMID:29221460)
Proper citation: RRID:Addgene_104912 Copy
Genetic Insert: multiple cloning site
Vector Backbone Description: Backbone Size:5059; Vector Backbone:pMS2; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Proper citation: RRID:Addgene_20320 Copy
Species: synthetic gene derived from Tn10 tetR
Genetic Insert: Pimyc-tetR#10
Vector Backbone Description: Backbone Size:5133; Vector Backbone:pTE-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Comments: There are minor discrepancies between depositor's and Addgene's sequence. Depositor is aware of these and thinks they should not have any affect on function.
Proper citation: RRID:Addgene_20318 Copy
Species: Escherichia coli
Genetic Insert: e. coli galactokinase
Vector Backbone Description: Backbone Size:10000; Vector Backbone:PMSG346; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20851171
Proper citation: RRID:Addgene_26958 Copy
Species: synthetic gene
Genetic Insert: P#38-luxAB
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27061 Copy
Species: synthetic gene
Genetic Insert: P#38-mcherry rfp-DAS+4
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27060 Copy
Species: synthetic gene
Genetic Insert: P#38-mcherry rfp
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27058 Copy
Species: synthetic gene
Genetic Insert: P#38-gfp-DAS+4
Vector Backbone Description: Backbone Size:6087; Vector Backbone:pTE-0X1-GCT6; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Comments: pTE-mcs derivative (Addgene plasmid# 20320) with GFP-DAS+4 under the control of a strong promoter. DAS+4 sequence is AANDENYSENYADAS at end of GFP.
Proper citation: RRID:Addgene_27057 Copy
Species: synthetic gene
Genetic Insert: P#38-luxA-FLAG-luxB-DAS+4
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27064 Copy
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