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Species: Homo sapiens
Genetic Insert: U6
Vector Backbone Description: Backbone Marker:Addgene plasmid 51283; Vector Backbone:pAluYa5-neo-TET; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19390602
Comments: U6 is tagged with the self splicing intron neo cassette such that G418 resistance will be obtained only if retrotransposition occurs.
Please note that the cassette is in the reverse orientation within the MCS relative to the full sequence--This has no functional consequence.
Proper citation: RRID:Addgene_51286 Copy
Species: Homo sapiens
Genetic Insert: L1 ORF2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4595; Vector Backbone:pBudCE4.1; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:21572950
Comments: This plasmid was created using the codon optimized L1RP as a source for the ORF2 coding sequence.
Proper citation: RRID:Addgene_51289 Copy
Species: Homo sapiens
Genetic Insert: L1
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript II; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21572950
Comments: L1 is tagged with neoI indicator cassette containing an inverted neomycin resistance gene. The SV40 promoter (SV40p) drives transcription of the neomycin gene which is disrupted by an intron in the opposite orientation that renders it non-functional. The intron is only spliced out from tagged L1 transcripts generated by the CMV promoter. When retrotransposition of the spliced L1 RNA occurs, the new insert will contain a functional neo gene that is expressed from the SV40 promoter.
The L1 open reading frames were codon optimized using Primo Optimum 3.4 (http://www.changbioscience.com/primo/primoo.html), which makes synonymous changes to optimal codons within the nucleotide sequence. A 63 bp inter-ORF with 92% sequence identity to L1RP was utilized for all constructs. Sequence changes to this region were generated to introduce restriction enzyme sites used for plasmid construction. The optimized nucleotide sequence is 74.9% identical to the sequence of L1RP.
Proper citation: RRID:Addgene_51288 Copy
Species: Homo sapiens
Genetic Insert: L1RE1
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19390602
Comments: L1 is tagged with the self splicing intron neo cassette such that G418 resistance will be obtained only if retrotransposition occurs.
Proper citation: RRID:Addgene_51284 Copy
Species: Homo sapiens
Genetic Insert: H2B-eYFP
Vector Backbone Description: Backbone Marker:Luigi Naldini; Backbone Size:6697; Vector Backbone:pRRLsin.cPPT.Tie1p.eGFP.wpre; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20856866
Comments: Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2.
Proper citation: RRID:Addgene_51293 Copy
Species: Homo sapiens
Genetic Insert: Lin28B
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951094
Comments: N-terminal deletion (70 amino acids)
Proper citation: RRID:Addgene_51374 Copy
Species: Homo sapiens
Genetic Insert: pri-let-7a-1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951094
Proper citation: RRID:Addgene_51377 Copy
Species: Homo sapiens
Genetic Insert: pri-miR-29b-1~29a
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19079265
Comments: Point mutations: insertion (1115bp), deletion (A47, T666) (Unnecessary region for miRNA biogenesis)
Proper citation: RRID:Addgene_51376 Copy
Species: Homo sapiens
Genetic Insert: Lin28B
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951094
Proper citation: RRID:Addgene_51373 Copy
Species: Homo sapiens
Genetic Insert: Lin28A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951094
Comments: H147A, H169A
Proper citation: RRID:Addgene_51372 Copy
Species: Homo sapiens
Genetic Insert: pri-let-7b
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951094
Proper citation: RRID:Addgene_51378 Copy
Species: Homo sapiens
Genetic Insert: Kinesin-associated protein 3
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16262723
Proper citation: RRID:Addgene_51413 Copy
Species: Homo sapiens
Genetic Insert: pri-let-7e
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951094
Proper citation: RRID:Addgene_51380 Copy
Species: Homo sapiens
Genetic Insert: DGCR8
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16963499
Proper citation: RRID:Addgene_51386 Copy
Species: Homo sapiens
Genetic Insert: DGCR8
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16963499
Proper citation: RRID:Addgene_51385 Copy
Species: Homo sapiens
Genetic Insert: Lin28A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703396
Proper citation: RRID:Addgene_51387 Copy
Species: Homo sapiens
Genetic Insert: pri-let-7g
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951094
Proper citation: RRID:Addgene_51381 Copy
Species: Homo sapiens
Genetic Insert: DGCR8
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16963499
Comments: Addgene Quality Control sequencing has found some discrepancies in the full plasmid sequence in the V5 tag and linker region of the plasmid. The V5 tag and DGCR8 insert are not effected.
Proper citation: RRID:Addgene_51383 Copy
Species: Homo sapiens
Genetic Insert: NM_001039802.1
Vector Backbone Description: Backbone Marker:Promega (modified to have 3' multi cloning sites); Vector Backbone:Cdc42 3'UTR; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19079265
Comments: miR-29 seed mutant (position: 578-584, 997-1003, TGCT->GTAG)
Proper citation: RRID:Addgene_51390 Copy
Species: Homo sapiens
Genetic Insert: p85alpha 3'UTR
Vector Backbone Description: Backbone Marker:Promega (modified to have 3' multi cloning sites); Vector Backbone:pGL3-UC; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19079265
Comments: miR-29 seed mutant (TGCT->GTAG)
Proper citation: RRID:Addgene_51393 Copy
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