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Species: Pseudomonas putida
Genetic Insert: AlkS(WT)
Vector Backbone Description: Backbone Size:3997; Vector Backbone:pACYC; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:22326628
Proper citation: RRID:Addgene_44382 Copy
Species: Pseudomonas putida
Genetic Insert: AlkSm4-3
Vector Backbone Description: Backbone Size:3984; Vector Backbone:pACYC; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:22326628
Proper citation: RRID:Addgene_44383 Copy
Genetic Insert: pTEt-Bxb1, pBAD-TP901
Vector Backbone Description: Vector Backbone:J64100; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23539178
Proper citation: RRID:Addgene_44456 Copy
Genetic Insert: flaA-GFP
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pTLP6; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:10447882
Comments: The ~1kb BamHI/SphI flaAGFP fragment from pflaG into the ~4.6kb BamHI/SphI fragment of the ColE1 replicon pTLP6.
Proper citation: RRID:Addgene_45476 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: S. cerevisiae Hsp82
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC-Duet1 and pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17908693
Proper citation: RRID:Addgene_41187 Copy
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pSR47S; Vector Types:Bacterial Expression, Bacterial allelic exchange vector with sacB; Bacterial Resistance:Chloramphenicol
Comments: Plasmid pSR47S Cm was constructed by removing the KmR region from pSR47S by PCR amplification of the residual plasmid, then cloning in the CmR gene. The CmR gene was amplified from a pMMB207 plasmid by PCR, with restriction sites that allowed cloning into pSR47S that lacked the KmR region.
Proper citation: RRID:Addgene_41892 Copy
Species: S. pyogenes
Genetic Insert: dCas9 (bacteria)
Vector Backbone Description: Vector Backbone:p15A vector; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23452860
Proper citation: RRID:Addgene_44249 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: PpsbAI::luxCDE, PpsbAI::luxAB
Vector Backbone Description: Vector Backbone:pAM1582; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17417005
Comments: NSII.1 vector
Lux Genes: luxAB & luxCDE
synthesizes luciferase and substrate in S. elongatus
SmaI--PvuII fragment containing PpsbAI::luxCDE was cloned into SmaI site of pAM1582. Orientation of PpsbAI::luxCDE is same as that of PpsbAI::luxAB. NS2.1, PpsbAI::luxCDE, PpsbAI::luxAB plasmid.
Proper citation: RRID:Addgene_40245 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Strain description: CsrA system knockout
Chromosomal changes: ΔCsrA, ΔCsrB, ΔCsrC, ΔglgCAP, ΔpgaABCD, +LacIq at galK
Proper citation: RRID:Addgene_37980 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17220888
Comments: Host strain for pHL32B
Proper citation: RRID:Addgene_37976 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17220888
Comments: Host strain for pHL32B
Strain description: ΔDam
Chromosomal changes: ΔDam
Proper citation: RRID:Addgene_37977 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17220888
Comments: Host strain for pHL32B
Strain description: Δ(Dam+OxyR)
Chromosomal changes: Δ(Dam+OxyR)
Proper citation: RRID:Addgene_37979 Copy
Genetic Insert: Bxb1-Excisionase
Vector Backbone Description: Backbone Size:2800; Vector Backbone:J64100; Vector Types:Synthetic Biology, BioBrick cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:22615351
Comments: In the reset circuit, PBAD controls expression of a polycistron encoding excisionase with a strong RBS followed by Bxb1 integrase with a GTG start codon to decrease its translation rate
and increase the excisionase to integrase ratio. This construct was cloned in a plasmid
with a regulated ColE1 origin of replication (J64100, 50-70 copies, chloramphenicol resistance).
Proper citation: RRID:Addgene_38207 Copy
Species: Homo sapiens
Genetic Insert: AASDHPPT
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pCOEX-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: http://www.thesgc.org/structures/2BYD/
Proper citation: RRID:Addgene_39192 Copy
Species: S. pyogenes
Genetic Insert: tracrRNA
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23761437
Proper citation: RRID:Addgene_46570 Copy
Species: S. pyogenes
Genetic Insert: tracrRNA
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23761437
Proper citation: RRID:Addgene_46569 Copy
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:4980; Vector Backbone:pBR322; Vector Types:sequencing vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_46858 Copy
Genetic Insert: aiiA
Vector Backbone Description: Vector Backbone:pZ vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:22178928
Proper citation: RRID:Addgene_48888 Copy
Species: Shewanella amazonensis SB2B
Genetic Insert: AS02_915
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49729 Copy
Species: E. coli
Genetic Insert: ACP transacylase
Vector Backbone Description: Backbone Size:3956; Vector Backbone:pACM4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23361000
Proper citation: RRID:Addgene_49809 Copy
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