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Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector Q-R
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 16 and 18 of EMMA platform.
Proper citation: RRID:Addgene_100659 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector L-W
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 11 and 23 of EMMA platform.
Proper citation: RRID:Addgene_100655 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector L-Y
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 11 and 25 of EMMA platform.
Proper citation: RRID:Addgene_100656 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector L-M
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 11 and 13 of EMMA platform.
Proper citation: RRID:Addgene_100652 Copy
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 19 of EMMA platform.
Proper citation: RRID:Addgene_100629 Copy
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 15 of EMMA platform.
Proper citation: RRID:Addgene_100625 Copy
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 16 of EMMA platform.
Proper citation: RRID:Addgene_100626 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector A-B
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: DNA element with no function. It is used as a bridge to fill in the gap between the backbone and parts placed in position 2 of EMMA platform.
Proper citation: RRID:Addgene_100638 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector B-C
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 1 and 3 of EMMA platform.
Proper citation: RRID:Addgene_100639 Copy
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 23 of EMMA platform.
Proper citation: RRID:Addgene_100633 Copy
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 20 of EMMA platform.
Proper citation: RRID:Addgene_100630 Copy
Species: Homo sapiens
Genetic Insert: RARRES3
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20100577
Proper citation: RRID:Addgene_100724 Copy
Species: Homo sapiens
Genetic Insert: C-KDEL
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: KDEL sequence at C terminal of the coding sequence cloned in position 7.
Proper citation: RRID:Addgene_100681 Copy
Species: Synthetic
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: Fluorescent reporter - Red - ?ex (nm) = 559 ?em (nm) = 600 . doi: 10.1038/nmeth.2171
Proper citation: RRID:Addgene_100687 Copy
Species: Homo sapiens
Genetic Insert: Pim1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23936194
Proper citation: RRID:Addgene_100722 Copy
Species: Homo sapiens
Genetic Insert: MTMR1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27018598
Comments: The MTMR1 construct is for structural determination and contains the PH-GRAM and PTPase domains (amino acids 95-607). PTPase activity was confirmed.
Proper citation: RRID:Addgene_100723 Copy
Species: Homo sapiens
Genetic Insert: PGKpA
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: mouse phosphoglucokinase polyA. Terminator.
Proper citation: RRID:Addgene_100683 Copy
Species: Synthetic
Genetic Insert: Insulator_FB
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: FII/BEAD-A (FB) element. (STEMCELLS2008;26:3257�3266 ). FII/BEAD-A (FB) contains the minimal enhancer-blocking components of the chicken b-globin 5'HS4 insulator and a homologous region from the human T-cell receptor BEAD-1 insulator
IMPORT NOTES: Special character found in field(Comments)
Proper citation: RRID:Addgene_100686 Copy
Genetic Insert: CMV promoter
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: Strong constitutive promoter : Human�cytomegalovirus�(CMV) immediate early enhancer and�promoter
IMPORT NOTES: Special character found in field(Comments)
Proper citation: RRID:Addgene_100690 Copy
Genetic Insert: PuroR
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: Puromycin resistance gene ecnoding a puromycin N-acetyl-transferase.
Proper citation: RRID:Addgene_100691 Copy
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