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Species: Other
Genetic Insert: HPV16 E6E7
Vector Backbone Description: Backbone Size:5874; Vector Backbone:pLXSN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1845902
Proper citation: RRID:Addgene_52394 Copy
Species: Other
Genetic Insert: NGFP+Z-fusion
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5641; Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15631464
Proper citation: RRID:Addgene_52734 Copy
Species: Other
Genetic Insert: link-NGFP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5641; Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15631464
Proper citation: RRID:Addgene_52732 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Mammalian Expression, CRISPR, xenopus and zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24873830
Comments: Please see the Schier lab website http://labs.mcb.harvard.edu/schier/ for detailed protocols about target site selection, sgRNA production, stop codon cassette design, Cas9 protein purification, injection and downstream analysis.
This plasmid was used to prepare and inject Cas9 mRNA into zebrafish zygotes.
Proper citation: RRID:Addgene_47322 Copy
Species: Other
Genetic Insert: TRANSPORT INHIBITOR RESPONSE 1
Vector Backbone Description: Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23150568
Comments: Nishimura et al., 2009, Nature Methods
Please note that this version contains a nuclear export signal. A related construct without the NES can be found at: http://www.addgene.org/64945/
Proper citation: RRID:Addgene_47328 Copy
Species: Other
Genetic Insert: minCMV-dTomato_pA
Vector Backbone Description: Vector Backbone:pcrBluntII-topo; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47321 Copy
Species: Other
Genetic Insert: Cas9m2
Vector Backbone Description: Vector Backbone:pcDNA3.3_TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47314 Copy
Species: Other
Genetic Insert: wild type immunity region of lambda phage with tsr-venus-ub-cI
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:4225; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22751020
Proper citation: RRID:Addgene_47311 Copy
Species: Other
Genetic Insert: Cas9m2-VP64
Vector Backbone Description: Vector Backbone:pcDNA3.3_TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47317 Copy
Species: Other
Genetic Insert: Cas9m3-VP64
Vector Backbone Description: Vector Backbone:pcDNA3.3_TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47318 Copy
Species: Other
Genetic Insert: Cas9m3
Vector Backbone Description: Vector Backbone:pcDNA3.3_TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47315 Copy
Species: Other
Genetic Insert: Endophilin II
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2t; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Has A/G mutation resulting in K256E change
Proper citation: RRID:Addgene_47409 Copy
Species: Other
Genetic Insert: Endophilin
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2tk; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9341169
Proper citation: RRID:Addgene_47408 Copy
Species: Other
Genetic Insert: N-WASP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4735; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11584276
Comments: Original cDNA was a gift from Dr. Hiroaki Miki
Proper citation: RRID:Addgene_47406 Copy
Species: Other
Genetic Insert: CLCb
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4961; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15533941
Proper citation: RRID:Addgene_47404 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23876708
Proper citation: RRID:Addgene_47516 Copy
Species: Other
Genetic Insert: Intersectin SH3 domain C
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2t; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9813051
Proper citation: RRID:Addgene_47398 Copy
Species: Other
Genetic Insert: Intersectin SH3 domain A
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2t; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9813051
Proper citation: RRID:Addgene_47396 Copy
Species: Other
Genetic Insert: Alpha A-crystallin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3716; Vector Backbone:pET-20b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479631
Comments: Gene was obtained from collaborator Andor Kiss, Miami University of Ohio.
Proper citation: RRID:Addgene_47666 Copy
Species: Other
Genetic Insert: Alpha A-crystallin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3716; Vector Backbone:pET-20b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479631
Proper citation: RRID:Addgene_47667 Copy
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