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Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (aa 345 - 468)
Vector Backbone Description: Backbone Marker:Cheeseman lab; Backbone Size:16397; Vector Backbone:pIC26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: MEX-5 transgenes driven by the pie-1 promoter and pie-1 3'UTR were constructed by cloning the mex-5 cDNA as a SpeI fragment downstream of a Dendra2/TEV/S-peptide tag cloned into pIC26 LAP tag (Cheeseman et al., 2004).
Alternate plasmid name: pIC26 Dendra + MEX-5 (aa345-STOP) (Dendra + MEX-5 ORF)
Proper citation: RRID:Addgene_40086 Copy
Species: Caenorhabditis elegans
Genetic Insert: GLH-1::nos-2 3’UTR
Vector Backbone Description: Backbone Marker:custom; Backbone Size:6700; Vector Backbone:pEV1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20335358
Proper citation: RRID:Addgene_37487 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-let-7
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:7066; Vector Backbone:MXW-PGK-IRES-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22142974
Proper citation: RRID:Addgene_37646 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-let-7
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:7066; Vector Backbone:MXW-PGK-IRES-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22142974
Proper citation: RRID:Addgene_37647 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-let-7
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:7066; Vector Backbone:MXW-PGK-IRES-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22142974
Proper citation: RRID:Addgene_37642 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-let-7
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:7066; Vector Backbone:MXW-PGK-IRES-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22142974
Proper citation: RRID:Addgene_37643 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-let-7
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:7066; Vector Backbone:MXW-PGK-IRES-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22142974
Proper citation: RRID:Addgene_37644 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-let-7
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:7066; Vector Backbone:MXW-PGK-IRES-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22142974
Proper citation: RRID:Addgene_37641 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pnhx-2cpl-1::YFP
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37834 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pnhx-2cpl-1W32A;Y35A::YFP
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37835 Copy
Species: Caenorhabditis elegans
Genetic Insert: F13D12.6(G166R)
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37837 Copy
Species: Caenorhabditis elegans
Genetic Insert: Ub-V
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37839 Copy
Species: Caenorhabditis elegans
Genetic Insert: cbr-unc-119::FRT*::galk::FRT*
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KSII(+); Vector Types:Worm Expression, recombineering; Bacterial Resistance:Ampicillin
Comments: This plasmid carries a complementary cassette consisting of a genomic copy of the C. briggsae unc-119 gene and the selectable galK marker flanked by FRT* sites in the presumptive 3’ UTR of the Cbr-unc-119 gene.
The 3.6 kb Cbr-unc-119::FRT*::galk::FRT* cassette can be targeted by recombineering to insert between nts 281:282 in the pCC1FOS fosmid vector backbone. In other words, this cassette can be readily inserted in all fosmids contained in the C. elegans library developed by Don Moerman and colleagues. We have tested the construct in fosmid recombineering and have shown that it rescues unc-119(ed3) mutants.
Proper citation: RRID:Addgene_38149 Copy
Species: Caenorhabditis elegans
Genetic Insert: ptc-3::GFP
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KSII(+); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21215260
Comments: The pPK348 plasmid was derived by inserting the GFP cassette from pPD103.87 (Addgene plasmid 1524) flanked by XbaI ends into the unique SpeI site present in exon 8 of ptc-3 and carries the entire predicted ptc-3 locus comprised of a 6.9 kb genomic coding region fused in-frame to GFP and more than 5 kb of 5′ upstream sequence, including the putative promoter
Please note that Addgene's sequencing results identified several mismatches in the region of bp#722-824 when compared to the full plasmid sequence provided by the depositing laboratory. The depositor states that these mismatches are not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_38145 Copy
Species: Caenorhabditis elegans
Genetic Insert: let-60p::ptc-3::gfp
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KSII(+); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21215260
Comments: The pPK700 plasmid was derived by ligating the let-60 promoter region to ptc-3::gfp expression cassette, which was PCR amplified from pPK348 (Addgene plasmid 38145)
Proper citation: RRID:Addgene_38146 Copy
Species: Caenorhabditis elegans
Genetic Insert: mir40-3
Vector Backbone Description: Backbone Marker:Bartel lab (Addgene plasmid# 46646); Backbone Size:8038; Vector Backbone:pcDNA3.2 V5-DEST mir-1-1 reporter; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23415231
Proper citation: RRID:Addgene_46698 Copy
Species: Caenorhabditis elegans
Genetic Insert: mir40-1
Vector Backbone Description: Backbone Marker:Bartel lab (Addgene plasmid# 46646); Backbone Size:8038; Vector Backbone:pcDNA3.2 V5-DEST mir-1-1 reporter; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23415231
Proper citation: RRID:Addgene_46696 Copy
Species: Caenorhabditis elegans
Genetic Insert: mir40-4
Vector Backbone Description: Backbone Marker:Bartel lab (Addgene plasmid# 46646); Backbone Size:8038; Vector Backbone:pcDNA3.2 V5-DEST mir-1-1 reporter; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23415231
Proper citation: RRID:Addgene_46699 Copy
Species: Caenorhabditis elegans
Genetic Insert: mir50-7
Vector Backbone Description: Backbone Marker:Bartel lab (Addgene plasmid# 46646); Backbone Size:8038; Vector Backbone:pcDNA3.2 V5-DEST mir-1-1 reporter; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23415231
Proper citation: RRID:Addgene_46716 Copy
Species: Caenorhabditis elegans
Genetic Insert: mir50-wt
Vector Backbone Description: Backbone Marker:Bartel lab (Addgene plasmid# 46646); Backbone Size:8038; Vector Backbone:pcDNA3.2 V5-DEST mir-1-1 reporter; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23415231
Proper citation: RRID:Addgene_46719 Copy
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