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Genetic Insert: coxM1′::lacZ reporter system with RcoM binding sites "a-f" spaced at 21bp AND RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX3254 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3254 (coxM1′::lacZ reporter system with binding sites “a-f” with a deletion between the “c” and “d” binding motifs such that the individual binding motifs “a,” “b,” “c,” “d,” “e,” and “f” are spaced at 21-bp intervals) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, pUX3254 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37178 Copy
Genetic Insert: coxM1′::lacZ reporter system of pUX3009 with an improved −35/ −10 RNAP binding region AND RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX3272 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3272 (pUX3009 based coxM1′::lacZ reporter system with binding sites “d + e + f” and an improved −35/−10 RNAP binding region (5′-TTGACA-(18N)-TATCCT-3′) ) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, pUX3272 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37179 Copy
Genetic Insert: coxM1′::lacZ reporter system of pUX3009 with “e” binding site “TTnnnG” motif changed to “GGnnnG” AND RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX3297 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3297 (pUX3009 based coxM1′::lacZ reporter system with binding sites “d + e + f” with the “e” binding site “TTnnnG” motif changed to “GGnnnG;”) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, pUX3297 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37180 Copy
Genetic Insert: coxM1′::lacZ reporter system with RcoM binding site "f" AND RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX3008 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3008 (coxM1′::lacZ reporter system with binding site “f”) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, pUX3008 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37169 Copy
Genetic Insert: coxM1′::lacZ reporter system AND Burkholderia xenovorans RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX2996 and pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX2996 (pUX2970 with a coxM1′::lacZ reporter system such that ...vector DNA – BamHI (unique site) – “e + f” binding region – coxM1′(with unique SalI site)::lacZ-> tfd <-KanR (with 5′-end unique XhoI site) – vector DNA <-SpR...) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, plasmid pUX2996 is KanR and SpecR and plasmid is pUX2410 is AmpR. Can be grown in media containing Amp + Kan.
For strain usage see attached pdf. Please note that this strain has not been tested functionally by Addgene.
Proper citation: RRID:Addgene_37167 Copy
Species: Burkholderia xenovorans
Genetic Insert: ~1200-bp fragment from Burkholderia xenovorans LB400 cloned into pEXT20
Vector Backbone Description: Vector Backbone:pUX2442; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:18326575
Comments: This strain contains Roberts lab plasmid pUX2442 which contains ~1200bp fragment from Burkholderia xenovorans LB400 with introduced terminal HindIII and SalI sites [such that HindIII – (<-rcoM1
– intergenic region – coxM1′->) – SalI] cloned into pEXT20 with the lacZ + KanR cassette from pKOK6 cloned at the SalI site such that coxM1′::lacZ->tfd<-KanR. Suitable template for amplification of the Burkholderia xenovorans RcoM1 binding region + reporter cassette.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37164 Copy
Genetic Insert: coxM1′::lacZ reporter system with RcoM binding sites "d + e + f" AND RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX3009 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3009 (coxM1′::lacZ reporter system with binding sites “d + e + f”) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, pUX3009 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37171 Copy
Genetic Insert: RAJ11 system (no transRNA)
Vector Backbone Description: Backbone Marker:Jaramillo Lab; Backbone Size:4042; Vector Backbone:pSTC0; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:22949707
Proper citation: RRID:Addgene_39245 Copy
Vector Backbone Description: Backbone Marker:Registry of standard biological parts; Backbone Size:4042; Vector Backbone:pSB1AK3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:22949707
Comments: This plasmid is used for inserting a 5'UTR in front of the GFP CDS using the NheI and EcoRi sites
Proper citation: RRID:Addgene_39240 Copy
Genetic Insert: pLlacO-1+BBa_B0034+GFP
Vector Backbone Description: Backbone Marker:Jaramillo Lab; Backbone Size:4042; Vector Backbone:pSTC0; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:22949707
Proper citation: RRID:Addgene_39242 Copy
Genetic Insert: RAJ22 system (no transRNA)
Vector Backbone Description: Backbone Marker:Jaramillo Lab; Backbone Size:4042; Vector Backbone:pSTC0; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:22949707
Proper citation: RRID:Addgene_39252 Copy
Genetic Insert: RAJ22 system
Vector Backbone Description: Backbone Marker:Jaramillo Lab; Backbone Size:4042; Vector Backbone:pSTC0; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:22949707
Proper citation: RRID:Addgene_39251 Copy
Genetic Insert: RAJ23 system
Vector Backbone Description: Backbone Marker:Jaramillo Lab; Backbone Size:4042; Vector Backbone:pSTC0; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:22949707
Proper citation: RRID:Addgene_39253 Copy
Vector Backbone Description: Vector Backbone:pJOE; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:34175462
Comments: Please note that the Addgene verified sequence differs from the depositor's reference sequence linked under the supplemental documents section. These differences do not alter the plasmid function.
Proper citation: RRID:Addgene_169872 Copy
Species: Other
Genetic Insert: OsTIR1
Vector Backbone Description: Vector Backbone:pDB4697; Vector Types:Other; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:34849776
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.19.452993v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171126 Copy
Species: Synthetic
Genetic Insert: GLT1 POL1 24xPP7SL
Vector Backbone Description: Vector Backbone:pHIS; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21512033
Comments: Note that Addgene NGS results found 22xPP7 stem loop repeats rather than expected 24. It is not known how this impacts plasmid function.
This plasmid was designed to construct mRNAs that contain multiple copies of an RNA sequence (the 24xPP7 stem-loops) that can be recognized by a chimeric RNA-binding protein fused to GFP, encoded in plasmid pDZ276 (Addgene plasmid #35194). See associated publication for detailed methods and use of this plasmid.
Please note that the LacZa-ccdB fusion in this plasmid is not functional and was originally present in the vector backbone to aid in screening for insert.
Proper citation: RRID:Addgene_35196 Copy
Species: Desulfovibrio vulgaris
Genetic Insert: uracil phosphoribosyltransferase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCR4/TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23393089
Comments: Selection for kanamycin resistance after introduction of pMO746 identifies those cells in which integration into the chromosome of part or all of the plasmid has occurred.
Kanamycin resistance is a strong selection in the sulfate-reducing bacteria (SRB). Ampicillin is not universally effective in the SRB but is often used for selection in Escherichia coli. Thus this plasmid can be readily grown in E. coli but is actually unstable in the SRB.
Proper citation: RRID:Addgene_117480 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: Km Resistance, neutral site II integration platform for Synechococcus
Vector Backbone Description: Vector Backbone:1572; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:10812624
Comments: NSII vector
cloning sites: NheI, XbaI, StuI, SalI, and EcoRV.
Km cassette into Klenow blunted BglII site of pAM1572. XhoI digest gives 0.7kb band. NSII vector; unique cloning sites NheI, XbaI, StuI, SalI, EcoRV.
Proper citation: RRID:Addgene_40240 Copy
Species: S. mediterranea
Genetic Insert: Protein arginine methyltransferase 1 homolog
Vector Backbone Description: Backbone Marker:Jim Collins, Newmark lab; Vector Backbone:pJC53.2; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:22318224
Comments: There are several mismatches between Addgene's quality control sequence and the available NCBI sequence. Depositing lab believes these mismatches will not affect plasmid function.
Proper citation: RRID:Addgene_35694 Copy
Species: S. mediterranea
Genetic Insert: Protein arginine methyltransferase 5
Vector Backbone Description: Backbone Marker:Jim Collins, Newmark lab; Vector Backbone:pJC53.2; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:22318224
Comments: There are several mismatches between Addgene's quality control sequence and the best match available NCBI sequence (AEX33822.1). Depositing lab believes these mismatches will not affect plasmid function.
Proper citation: RRID:Addgene_35692 Copy
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