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Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Proper citation: RRID:Addgene_234588 Copy
Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Vector Backbone:pMB.001; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Proper citation: RRID:Addgene_234574 Copy
Species: Homo sapiens
Genetic Insert: SUZ12
Vector Backbone Description: Vector Backbone:pMB.001; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Proper citation: RRID:Addgene_234575 Copy
Species: Homo sapiens
Genetic Insert: RBBP4
Vector Backbone Description: Vector Backbone:pMB.001; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Proper citation: RRID:Addgene_234577 Copy
Species: Homo sapiens
Genetic Insert: AEBP2 [Isoform 3]
Vector Backbone Description: Vector Backbone:pMB.001; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Proper citation: RRID:Addgene_234578 Copy
Species: Homo sapiens
Genetic Insert: AEBP2iso2.mt2A
Vector Backbone Description: Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247009 Copy
Species: Homo sapiens
Genetic Insert: AEBP2iso1
Vector Backbone Description: Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247005 Copy
Species: Homo sapiens
Genetic Insert: AEBP2iso2.mt1A
Vector Backbone Description: Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247006 Copy
Species: Homo sapiens
Genetic Insert: AEBP2iso2.mt1K
Vector Backbone Description: Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247007 Copy
Species: Homo sapiens
Genetic Insert: AEBP2iso2.mt1A2A
Vector Backbone Description: Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247012 Copy
Species: Homo sapiens
Genetic Insert: AEBP2iso2.mt1K2K
Vector Backbone Description: Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247013 Copy
Species: Homo sapiens
Genetic Insert: AEBP2iso2.mt1∆2∆
Vector Backbone Description: Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247014 Copy
Species: Homo sapiens
Genetic Insert: JARID2
Vector Backbone Description: Vector Backbone:pMB.Pos6; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247018 Copy
Species: Homo sapiens
Genetic Insert: Dipeptidyl peptidase 9
Vector Backbone Description: Backbone Size:4683; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39552560
Proper citation: RRID:Addgene_254905 Copy
Species: Homo sapiens
Genetic Insert: Dipeptidyl peptidase 9
Vector Backbone Description: Backbone Size:4683; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39552560
Proper citation: RRID:Addgene_254906 Copy
Genetic Insert: SAGE cassette
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18T-mini-Tn7T-Gm; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Comments: A C to T mutation at nt 33 of SAGE cassette was found, this does not impact the intended use of the plasmid.
Alternative name: pRC414.v2
Usage/Comments: This plasmid is used to generate a cSAGE-compatible base strain by integrating the poly-attB landing pad cassette into the host genome using a Tn7-based strategy. The landing pad contains multiple orthogonal serine recombinase attachment sites (attB), enabling subsequent site-specific integration of genetic payloads via cSAGE. Once integrated, the host strain becomes compatible with Bxb1, R4, TG1, and other serine recombinases for modular genome engineering. This plasmid is used only for installation of the landing pad and is not required for downstream integration steps after the base strain is constructed. Please visit https://doi.org/10.64898/2026.04.17.717921 for bioRxiv preprint.
Proper citation: RRID:Addgene_251503 Copy
Species: Pseudomonas aeruginosa PA14
Genetic Insert: Type IF CRISPR-Cas system from Pseudomonas aeruginosa PA14
Vector Backbone Description: Backbone Marker:Herbert Schweizer (Addgene #63121); Backbone Size:4500; Vector Backbone:pUC18T-mini-Tn7T-Gm; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40904105
Comments: Synthetic Type IF CRISPR-Cas system based on the sequence from Pseudomonas aeruginosa PA14 with a minimised version of the CRISPR 2 array containing a non-targeting spacer with BbsI restriction sites for cloning. Native promoter regions were cloned as the intergenic region between the CRISPR components and upstream genes. MluI, NsiI, NotI, NcoI, AscI restriction sites were added to make the system modular.
Proper citation: RRID:Addgene_246943 Copy
Vector Backbone Description: Backbone Size:5279; Vector Backbone:pFBDM; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:17117155
Comments: pFBDM was derived from pFastBacDUAL (Invitrogen), which enables the production of recombinant proteins in insect cells by baculovirus. This pFBDM vector is used mostly in our lab for the production of various target proteins composed of one or two polypeptides.
Proper citation: RRID:Addgene_110738 Copy
Species: Synthetic
Genetic Insert: SpyTag-noro-VLP
Vector Backbone Description: Backbone Marker:Gibco; Backbone Size:5238; Vector Backbone:pFastBac Dual; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:33468139
Comments: Original publication:
SpyCatcher:
Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. doi: 10.1073/pnas.1115485109. Epub 2012 Feb 24. PMID: 22366317; PMCID: PMC3311370.
Proper citation: RRID:Addgene_165989 Copy
Species: Scytonema hofmanni
Genetic Insert: tnsB
Vector Backbone Description: Vector Backbone:R6K conditional origin, bla, RP4 oriT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:34873292
Comments: Clone guides using the 2xBsaI cloning site.
Clone Tn barcodes using the 2xBsmBI cloning site.
Proper citation: RRID:Addgene_186692 Copy
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