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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pChlr-8SE
 
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RRID:Addgene_52172 Pumilio homology domain amino acids 284-319 Homo sapiens Chloramphenicol PMID:24581042 Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol Changed 12N to 12S and 16Q to 16E in module 8 2026-08-15 01:16:25 0
pChlr-8NQ
 
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RRID:Addgene_52173 Pumilio homology domain amino acids 284-319 Homo sapiens Chloramphenicol PMID:24581042 Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:16:21 0
pIM1773-PRHIII-sfGFP-pNW33N
 
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RRID:Addgene_52217 PRHIII-sfGFP Synthetic Chloramphenicol PMID:24788326 Plasmid is designed for use in Geobacillus stearothermophilus NUB3621 (as well as E. coli.) Construct is described in the article linked at the bottom of this page. This plasmid is a companion to pIM1708-PsurP-surT-agaN-pNW33N which was previously submitted. As with pIM1708, despite the fact that your plasmid map displays an ampicillin resistance gene, the correct antibiotic resistance gene is a chloramphenicol acetyltransferase. We do not know if the ampicillin resistance gene is functional. Backbone Marker:Bacillus Genetic Stock Center; Backbone Size:4217; Vector Backbone:pNW33N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:16:26 0
pAC-VIOL
 
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RRID:Addgene_53087 zep Arabidopsis thaliana Chloramphenicol PMID:24506237 For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:10040; Vector Backbone:pAC-ZEAXipi; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol Lacks codons for the first 60 N-terminal amino acids that are thought to comprise the chloroplast transit sequence 2026-08-15 01:16:32 0
pWJ66
 
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1+ mentions
RRID:Addgene_46570 tracrRNA S. pyogenes Chloramphenicol PMID:23761437 Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol 2026-08-15 01:15:33 4
pdCas9
 
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10+ mentions
RRID:Addgene_46569 tracrRNA S. pyogenes Chloramphenicol PMID:23761437 Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol 2026-08-15 01:15:33 17
pINIT_cat
 
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1+ mentions
RRID:Addgene_46858 Chloramphenicol PMID:21410291 Backbone Marker:unknown; Backbone Size:4980; Vector Backbone:pBR322; Vector Types:sequencing vector; Bacterial Resistance:Chloramphenicol 2026-08-15 01:15:36 6
pAC-EsaR-EsaI
 
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RRID:Addgene_47660 esaI-term Chloramphenicol PMID:23879176 esaI is 636 bp and indicated as lowercase letters in the partial sequence (between BamHI and XhoI sites). See pAC-EsaR for lac promoter and esaR sequence information. Backbone Size:4511; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:15:43 0
pAC-EsaR-I70V/D91G
 
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RRID:Addgene_47644 esaR-I70V/D91G Chloramphenicol PMID:23363022 esaR-I70V/D91G (750 bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map. Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol Changed Isoleucine 70 to Valine and Aspartic acid 91 to Glycine 2026-08-15 01:15:43 0
pAC-EsaR-I70V/D91G/V220A
 
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RRID:Addgene_47647 esaR-I70V/D91G/V220A Chloramphenicol PMID:23363022 esaR-I70V/D91G/V220A (750bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map. Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol Changed Isoleucine 70 to Valine, Aspartic acid 91 to Glycine, and Valine 220 to Alanine 2026-08-15 01:15:43 0
pAC-EsaR-V220A
 
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RRID:Addgene_47645 esaR-V220A Chloramphenicol PMID:23363022 esaR-V220A (750bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map. Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol Changed Valine 220 to Alanine 2026-08-15 01:15:43 0
pAC-EsaR-D91G
 
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RRID:Addgene_47646 esaR-D91G Chloramphenicol PMID:23363022 esaR-D91G (750bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in pAC-EsaR in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map. Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol Changed Aspartic acid 91 to Glycine 2026-08-15 01:15:44 0
pAC-NEUR
 
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RRID:Addgene_53271 crtI Rhodobacter capsulatus Chloramphenicol PMID:7919981 For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:8106; Vector Backbone:pAC-PHYT; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol 2026-08-15 01:16:29 0
pAC-DELTA
 
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1+ mentions
RRID:Addgene_53273 lcyE Arabidopsis thaliana Chloramphenicol PMID:8798688 For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:9476; Vector Backbone:pAC-LYC; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol 2026-08-15 01:16:32 1
pAC-ZETA
 
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1+ mentions
RRID:Addgene_53316 pds Synechococcus PCC7942 Chloramphenicol PMID:7919981 For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:8106; Vector Backbone:pAC-PHYT; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol 2026-08-15 01:16:29 1
pAC-85b
 
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1+ mentions
RRID:Addgene_53282 crtE, crtY, crtI Erwinia herbicola (Pantoea agglomerans) Eho10 Chloramphenicol PMID:17634749 For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. Backbone Marker:New England BioLabs; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol 2026-08-15 01:16:32 4
pAC-PHYTipi
 
Resource Report
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1+ mentions
RRID:Addgene_53283 crtE, idi, crtY, crtI, crtB Erwinia herbicola Eho10 Chloramphenicol PMID:17634749 For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:13581; Vector Backbone:pAC-EHER; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol 2026-08-15 01:16:29 1
pAC-94N
 
Resource Report
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1+ mentions
RRID:Addgene_53281 crtY, crtI, crtB Erwinia herbicola Eho10 Chloramphenicol PMID:17634749 For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:10609; Vector Backbone:pAC-BETA; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol 2026-08-15 01:16:29 1
pAC-ZETAipi
 
Resource Report
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1+ mentions
RRID:Addgene_53284 pds Synechococcus PCC7942 Chloramphenicol PMID:17634749 For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:13581; Vector Backbone:pAC-EHER; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol 2026-08-15 01:16:29 1
pAC-CANTHipi
 
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1+ mentions
RRID:Addgene_53301 crtO Haematococcus pluvialis Chloramphenicol PMID:17634749 For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:9259; Vector Backbone:pAC-BETAipi; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol 2026-08-15 01:16:29 9

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