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Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 284-319
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52172 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 284-319
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52173 Copy
Species: Synthetic
Genetic Insert: PRHIII-sfGFP
Vector Backbone Description: Backbone Marker:Bacillus Genetic Stock Center; Backbone Size:4217; Vector Backbone:pNW33N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24788326
Comments: Plasmid is designed for use in Geobacillus stearothermophilus NUB3621 (as well as E. coli.) Construct is described in the article linked at the bottom of this page. This plasmid is a companion to pIM1708-PsurP-surT-agaN-pNW33N which was previously submitted. As with pIM1708, despite the fact that your plasmid map displays an ampicillin resistance gene, the correct antibiotic resistance gene is a chloramphenicol acetyltransferase. We do not know if the ampicillin resistance gene is functional.
Proper citation: RRID:Addgene_52217 Copy
Species: Arabidopsis thaliana
Genetic Insert: zep
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:10040; Vector Backbone:pAC-ZEAXipi; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24506237
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53087 Copy
Species: S. pyogenes
Genetic Insert: tracrRNA
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23761437
Proper citation: RRID:Addgene_46570 Copy
Species: S. pyogenes
Genetic Insert: tracrRNA
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23761437
Proper citation: RRID:Addgene_46569 Copy
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:4980; Vector Backbone:pBR322; Vector Types:sequencing vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_46858 Copy
Genetic Insert: esaI-term
Vector Backbone Description: Backbone Size:4511; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23879176
Comments: esaI is 636 bp and indicated as lowercase letters in the partial sequence (between BamHI and XhoI sites). See pAC-EsaR for lac promoter and esaR sequence information.
Proper citation: RRID:Addgene_47660 Copy
Genetic Insert: esaR-I70V/D91G
Vector Backbone Description: Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23363022
Comments: esaR-I70V/D91G (750 bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map.
Proper citation: RRID:Addgene_47644 Copy
Genetic Insert: esaR-I70V/D91G/V220A
Vector Backbone Description: Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23363022
Comments: esaR-I70V/D91G/V220A (750bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map.
Proper citation: RRID:Addgene_47647 Copy
Genetic Insert: esaR-V220A
Vector Backbone Description: Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23363022
Comments: esaR-V220A (750bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map.
Proper citation: RRID:Addgene_47645 Copy
Genetic Insert: esaR-D91G
Vector Backbone Description: Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23363022
Comments: esaR-D91G (750bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in pAC-EsaR in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map.
Proper citation: RRID:Addgene_47646 Copy
Species: Rhodobacter capsulatus
Genetic Insert: crtI
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:8106; Vector Backbone:pAC-PHYT; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:7919981
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53271 Copy
Species: Arabidopsis thaliana
Genetic Insert: lcyE
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:9476; Vector Backbone:pAC-LYC; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:8798688
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53273 Copy
Species: Synechococcus PCC7942
Genetic Insert: pds
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:8106; Vector Backbone:pAC-PHYT; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:7919981
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53316 Copy
Species: Erwinia herbicola (Pantoea agglomerans) Eho10
Genetic Insert: crtE, crtY, crtI
Vector Backbone Description: Backbone Marker:New England BioLabs; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17634749
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53282 Copy
Species: Erwinia herbicola Eho10
Genetic Insert: crtE, idi, crtY, crtI, crtB
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:13581; Vector Backbone:pAC-EHER; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17634749
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53283 Copy
Species: Erwinia herbicola Eho10
Genetic Insert: crtY, crtI, crtB
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:10609; Vector Backbone:pAC-BETA; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17634749
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53281 Copy
Species: Synechococcus PCC7942
Genetic Insert: pds
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:13581; Vector Backbone:pAC-EHER; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17634749
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53284 Copy
Species: Haematococcus pluvialis
Genetic Insert: crtO
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:9259; Vector Backbone:pAC-BETAipi; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17634749
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53301 Copy
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