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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pTE-4S-0X Resource Report Resource Website |
RRID:Addgene_17973 | tetR(B)r1.7 | Hygromycin | PMID:17483222 | revTetR is expressed from a strong mycobacterial promoter (psmyc = puv15). Also see Pubmed ID 18059281. Derivative of pMS2 with tetR r1.7 expressed by Psmyc. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators. | Backbone Size:0; Vector Backbone:pMS2; Vector Types:mycobacteria expression; Bacterial Resistance:Hygromycin | 2026-08-15 01:11:23 | 0 | ||
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pUV15tetORm Resource Report Resource Website 1+ mentions |
RRID:Addgene_17975 | pmyc1tetO-gfp, tetR(B) | Hygromycin | PMID:17483222 | Expresses GFP under control of tetR(B). Also see Pubmed IDs 18059281 and 15687379. pUV15 derivative, Genotype: Hygr, Kmr, pimyc-tetR, pmyc1 tetO-gfp+ | Backbone Size:0; Vector Backbone:pMS2; Vector Types:mycobacteria expression; Bacterial Resistance:Hygromycin | 2026-08-15 01:11:25 | 2 | ||
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pML2424 Resource Report Resource Website |
RRID:Addgene_117497 | Hygromycin | PMID:22885758 | Addgene QC Next-generation sequencing identified sequence discrepancies relative to the Genbank file linked under Supplemental Documents. Plasmid is expected to function as described in the associated publication. | Vector Backbone:pML2424; Vector Types:Deletion generation; Bacterial Resistance:Hygromycin | 2026-08-15 01:02:54 | 0 | |||
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pYUB1062-GFP Resource Report Resource Website |
RRID:Addgene_117695 | Hygromycin | PMID:30319771 | This is a shuttle vector, designed for expression of the inserted gene in Mycobacterium smegmatis as well as allowing cloning and expressions using E. coli. This plasmid was constructed by Anajana Radhakrishnan from the pYUB1062 vector. pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein College of Medicine, New York, USA. References: this paper has been accepted in RSC Advances: A GFP-strategy for efficient recombinant protein overexpression and purification in Mycobacterium smegmatis: Anjana Radhakrishnan, Christopher M Furze, Mohd Syed Ahangar, Elizabeth Fullam | Backbone Marker:pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein; Backbone Size:5800; Vector Backbone:pYUB1062; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin | 2026-08-15 01:02:56 | 0 | |||
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pMEG_BB3_YL18H_AC Resource Report Resource Website |
RRID:Addgene_117828 | Hygromycin | PMID:30698703 | Contains 34 bp GoldenMOCS-Yali Linker AB | Backbone Size:4649; Vector Backbone:pMEG_BB3_YL18H_AC; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Hygromycin | 2026-08-15 01:02:58 | 0 | |||
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pMST-1313 Resource Report Resource Website |
RRID:Addgene_118076 | pTEF1-cexA-tCYC1 | A. niger | Hygromycin | PMID:30553933 | Backbone Marker:Matthias Steiger; Vector Backbone:MST606; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin | 2026-08-15 01:03:00 | 0 | ||
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pBSV2H Resource Report Resource Website |
RRID:Addgene_118229 | Hygromycin | PMID:33257311 | Please visit https://www.biorxiv.org/content/early/2018/09/21/363390 for bioRxiv preprint. Addgene QC identified a partial duplication of the segment of the plasmid containing the HygR selectable marker. The plasmid is functional for transformation and selection in B. burgdorferi. This plasmid was functional for use as described in Takacs et al. 2018. | Vector Backbone:pBSV2H; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin | 2026-08-15 01:03:01 | 0 | |||
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pMyC Resource Report Resource Website 1+ mentions |
RRID:Addgene_42192 | Hygromycin | PMID:33006405 | Vector constructed by Dr. Arie Geerlof at EMBL-Hamburg (now at Helmholtz Zentrum München) | Backbone Marker:Daugelat et al 2003; Backbone Size:6900; Vector Backbone:pSD26; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin | 2026-08-15 01:14:51 | 6 | |||
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pRD393 Resource Report Resource Website |
RRID:Addgene_168774 | mEos3.2_H-NSdbd | Synthetic | Hygromycin | PMID:34734265 | Backbone Size:5625; Vector Backbone:pFPV27; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin | 2026-08-15 01:09:56 | 0 | ||
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pRD445 Resource Report Resource Website |
RRID:Addgene_168775 | mEos3.2 | Synthetic | Hygromycin | PMID:34734265 | Backbone Size:5625; Vector Backbone:pFPV27; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin | 2026-08-15 01:09:55 | 0 | ||
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pSC301 Resource Report Resource Website 1+ mentions |
RRID:Addgene_31851 | GFP (including ATG start codon) | Hygromycin | PMID:11250077 | Plasmid can also be grown in mycobacteria. | Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:5497; Vector Backbone:pSODIT; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin | 2026-08-15 01:13:36 | 3 | ||
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pALACE Resource Report Resource Website |
RRID:Addgene_31853 | Hygromycin | PMID:12754249 | Plasmid can also be grown in mycobacteria. Also see PMID 15342574 for additional reference. Please note that Addgene were unable to verify the His tag in this construct. In addition there is a 1bp mismatch in our ACEpro-F sequence with the available NCBI reference. Depositing lab believes the construct is functional. | Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:8221; Vector Backbone:pACE; Vector Types:Bacterial Expression, Escherichia coli-Mycobacterium shuttle plasmid; Bacterial Resistance:Hygromycin | 2026-08-15 01:13:40 | 0 | |||
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pSC300 Resource Report Resource Website 1+ mentions |
RRID:Addgene_31820 | GFP (lacking ATG start codon) | Hygromycin | PMID:11250077 | Plasmid can also be grown in mycobacteria. | Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:5497; Vector Backbone:pSODIT; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin | 2026-08-15 01:13:36 | 1 | ||
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pMN252 Resource Report Resource Website |
RRID:Addgene_32177 | rpsL, FRT-hyg-FRT | Hygromycin | PMID:15563844 | This is a suicide vector for gene deletions in mycobacteria; requires rpsL mutant (streptomycin resistant) | Vector Backbone:pMN250; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin | 2026-08-15 01:13:43 | 0 | ||
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pML1361 Resource Report Resource Website |
RRID:Addgene_32379 | int Ms6; gfpm2+; xylEm; ttsbiA, ttsbiB | Hygromycin | PMID:20692326 | This is an improved Ms6 integration vector for stable gene integration in mycobacteria with codon optimized gfp and xylE expression as reporter genes; terminators to protect expression cassette against transcriptional interference. Please note that the conserved N->S point mutation found in the Addgene QC sequence of the Ms6 integrase gene is of no consequence and the plasmid is still fully functional for integration. | Vector Backbone:pMl603; Vector Types:bacterial integration vector; Bacterial Resistance:Hygromycin | 2026-08-15 01:13:45 | 0 | ||
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pML1357 Resource Report Resource Website 1+ mentions |
RRID:Addgene_32378 | int Giles; gfpm2+; xylEm; ttsbiA, ttsbiB | Hygromycin | PMID:20692326 | This is an improved Giles integration vector for stable gene integration in mycobacteria with codon optimized gfp and xylE as reporter genes; terminators to protect expression cassette against transcriptional interference. | Vector Backbone:pML603; Vector Types:bacterial integration vector; Bacterial Resistance:Hygromycin | 2026-08-15 01:13:40 | 2 | ||
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pMycVec2 Resource Report Resource Website |
RRID:Addgene_32361 | pJAZ38 ori | Hygromycin | PMID:11707328 | This is a low copy number (one?) plasmid that co-replicates with pAL5000-derived plasmids in mycobacteria. | Vector Backbone:pMS2; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin | 2026-08-15 01:13:45 | 0 | ||
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pMS82 Resource Report Resource Website |
RRID:Addgene_68110 | aac3(IV) | E. coli | Hygromycin | PMID:12923110 | For research using this plasmid, please acknowledge the depositing laboratory and associated citation. | Vector Backbone:pSET152; Vector Types:Streptomyces integrating vector; Bacterial Resistance:Hygromycin | Fragment | 2026-08-15 01:18:40 | 0 |
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pTEC22 Resource Report Resource Website 1+ mentions |
RRID:Addgene_30181 | Mycobacterium Strong Promoter (MSP) | M. marinum | Hygromycin | PMID:22840407 | This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with tdKatuschka2. The Katuschka tandem-dimer ORF is present at bp# 44-1522 | Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin | 2026-08-15 01:13:25 | 4 | |
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pTEC21 Resource Report Resource Website |
RRID:Addgene_30180 | Mycobacterium Strong Promoter (MSP) | M. marinum | Hygromycin | This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Kaede. The Kaede ORF is present at bp# 5624-6304. | Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin | 2026-08-15 01:13:23 | 0 |
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