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Species: Danio rerio
Genetic Insert: vgll4a
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_254513 Copy
Species: Danio rerio
Genetic Insert: vgll4b_TDU2
Vector Backbone Description: Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Comments: Please note: This plasmid contains a D29E mutation in vgll4b. This mutation is not known to impact plasmid function.
Proper citation: RRID:Addgene_254509 Copy
Species: Danio rerio
Genetic Insert: chimeric zebrafish flt1 enhancer element
Vector Backbone Description: Vector Backbone:pDONR-P4-P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_261113 Copy
Species: Danio rerio
Genetic Insert: mir-206-1/mir-133b
Vector Backbone Description: Backbone Marker:Bartel Lab; Backbone Size:0; Vector Backbone:pBSII SK+ modified to introduce GFP and more cloning sites; Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19240133
Comments: For polycistronic mir-206-1/mir-133b, we were not able to identify the transcriptional start precisely by 5′-RACE due to the repetitive sequence in this region. Analyses of EST clones (BM259633, CF999541, CF348604, DR729161) mapped the approximate start of the mir-206-1/mir-133b primary transcript to 1.4 kb from the mir-206 hairpin. A 4.5-kb upstream fragment that includes ∼0.9 kb of the primary transcript was amplified from genomic DNA using primers 3 and 4 (5'-CTGCCCCATTTAGACTCTTTCTTGACG-3' and 5'-CCAGAAGAACCCAGACGAAACCCAC-3'). The promoter fragment was fused to a GFP reporter gene. The miRNA promoter fusion was flanked by I-SceI meganuclease recognition sites to increase the efficiency of transgenensis.
Please note that Addgene's sequencing results show some differences from the published sequence. The depositing lab is aware of the differences.
Proper citation: RRID:Addgene_20793 Copy
Species: Danio rerio
Genetic Insert: mir-124
Vector Backbone Description: Backbone Marker:Bartel Lab; Backbone Size:0; Vector Backbone:pBSII SK+ modified to introduce GFP and more cloning sites; Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19240133
Comments: 5′-RACE (GenRacer kit, Invitrogen) identified a transcriptional start of the mir-124-5 primary transcript, which is located 1.1 kb from the mir-124-5 hairpin. A 5.6-kb fragment upstream of the mir-124-5 transcriptional start was amplified from genomic DNA using primers 1 and 2 (5'-TGCTTTGAGGCACGGTTATGGTCCC-3' and 5'-CTCGGGCAGCGGCTTTTTGGTCCTG-3'). The promoter fragment was fused to a GFP reporter gene. The miRNA promoter fusion was flanked by I-SceI meganuclease recognition sites to increase the efficiency of transgenensis.
Proper citation: RRID:Addgene_20792 Copy
Species: Danio rerio
Genetic Insert: pBS119d10L
Vector Backbone Description: Backbone Marker:Strategene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12167406
Comments: To view partial vector sequence from the depositing lab, please click on the Reviews link.
Proper citation: RRID:Addgene_22081 Copy
Species: Danio rerio
Genetic Insert: vegf
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12110173
Proper citation: RRID:Addgene_22417 Copy
Species: Danio rerio
Genetic Insert: npas4l
Vector Backbone Description: Backbone Size:4063; Vector Backbone:pCS2; Vector Types:mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36044573
Comments: Addgene NGS result had deletion of amino acids 349-352 (TSSS). Depositor confirmed that this deletion does not affect the plasmid function.
Proper citation: RRID:Addgene_164654 Copy
Species: Danio rerio
Genetic Insert: ENSDARG00000024546
Vector Backbone Description: Backbone Size:4373; Vector Backbone:pHat2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34969839
Proper citation: RRID:Addgene_187001 Copy
Species: Danio rerio
Genetic Insert: ENSDARG00000024546
Vector Backbone Description: Backbone Size:4373; Vector Backbone:pHat2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34969839
Proper citation: RRID:Addgene_187185 Copy
Species: Danio rerio
Genetic Insert: ENSDARG00000024546
Vector Backbone Description: Backbone Size:4137; Vector Backbone:pHat2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34969839
Proper citation: RRID:Addgene_187188 Copy
Species: Danio rerio
Genetic Insert: Il34
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Vertebrate expression (zebrafish, chicken, xenopus); Bacterial Resistance:Ampicillin
Defining Citation: PMID:34979548
Proper citation: RRID:Addgene_168112 Copy
Species: Danio rerio
Genetic Insert: Csf1a
Vector Backbone Description: Backbone Size:9748; Vector Backbone:pAcGP-67B; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34979548
Proper citation: RRID:Addgene_168103 Copy
Species: Danio rerio
Genetic Insert: N22_HA_dreAdar1_E488Q
Vector Backbone Description: Vector Backbone:pCS2; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41653923
Proper citation: RRID:Addgene_250533 Copy
Species: Danio rerio
Genetic Insert: drePum1_HA_dreAdar1
Vector Backbone Description: Vector Backbone:pCS2; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41653923
Proper citation: RRID:Addgene_250541 Copy
Species: Danio rerio
Genetic Insert: brd9
Vector Backbone Description: Backbone Size:4817; Vector Backbone:pCS2+; Vector Types:Brd9(zebrafish)-EGFP reporter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41326797
Proper citation: RRID:Addgene_248787 Copy
Species: Danio rerio
Genetic Insert: CBS pair domain of zebrafish CNNM3
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_250563 Copy
Species: Danio rerio
Genetic Insert: CBS pair domain of zebrafish CNNM1
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_250561 Copy
Species: Danio rerio
Genetic Insert: zebrafish PRL3
Vector Backbone Description: Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_250560 Copy
Species: Danio rerio
Genetic Insert: E1b:eGFP
Vector Backbone Description: Backbone Size:3873; Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37367831
Comments: A. Emelyanov and S. Parinov. Mifepristone-inducible LexPR system to drive and control gene expression in transgenic zebrafish. Developmental Biology, 320(1):113– 121, 2008. ISSN 00121606. doi: 10.1016/j.ydbio.2008.04.042.
A. Emelyanov, Y. Gao, N. I. Naqvi, and S. Parinov. Trans-kingdom transposition of the maize Dissociation element. Genetics, 174(3):1095–1104, 2006. ISSN 00166731. doi: 10.1534/genetics.106.061184.
Please visit https://www.biorxiv.org/content/10.1101/450684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_102417 Copy
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