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  • Organism:e. coli (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pHL 1571
 
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RRID:Addgene_37760 ptet_ompC_gfpAaV E. coli Ampicillin PMID:22618873 Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Synthetic Biology, E. coli; Bacterial Resistance:Ampicillin AaV degradation tag added to the 3'end 2026-08-15 01:14:16 0
pHL 1569
 
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RRID:Addgene_37759 ptet_ompC_gfpAaV E. coli Ampicillin PMID:22618873 Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Synthetic Biology, E. coli; Bacterial Resistance:Ampicillin AaV degradation tag added to the 3'end 2026-08-15 01:14:16 0
pET21-Streptavidin-Glutamate_Tag
 
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10+ mentions
RRID:Addgene_46367 Streptavidin-Glutamate_Tag E. coli Ampicillin PMID:24056174 Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:31 14
pBMN ltDHFR(DD)-YFP
 
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1+ mentions
RRID:Addgene_47076 ltDHFR(DD) E. coli Ampicillin PMID:23991108 This N-terminal ltDHFR(DD) plasmid is the clone#22 in Table 1 Backbone Marker:Garry Nolan ; Backbone Size:6943; Vector Backbone:pBMN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin amino acids mutations: R12H, N23S, G67S, V78A, E120G, E134G, E153V, E157G 2026-08-15 01:15:37 3
C321.ΔA
 
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10+ mentions
RRID:Addgene_48998 Recoded E. coli genome with all instances of the UAG codon and RF1 removed (UAG termination function removed) E. coli Ampicillin PMID:24136966 E. coli MG1655 Delta(ybhB-bioAB)::[lcI857 N(cro-ea59)::tetR-bla] Delta prfA Delta mutS::zeoR; all 321 UAG codons changed to UAA This strain is mutS-, which causes a spontaneous mutation frequency of ~2E-8. This strain is auxotrophic for d-biotin. A mutS+ version of this strain will be deposited with addgene soon. Genome sequence deposited at NCBI (accession # CP006698) Vector Backbone:E. coli genome; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin See genbank file 2026-08-15 01:15:56 13
C321.ΔA.exp
 
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10+ mentions
RRID:Addgene_49018 Recoded E. coli with all UAG codons and RF1 removed (UAG termination abolished); decreased mutation rate; 37C growth tolerated E. coli Bleocin (Zeocin) PMID:24136966 E. coli MG1655 Delta(ybhB-bioAB)::zeoR Delta prfA; all 321 UAG codons changed to UAA This strain is mutS+, which causes a normal spontaneous mutation frequency of ~1E-10. This strain is auxotrophic for d-biotin. The genome sequence of a similar strain (C321.deltaA) was deposited at NCBI (accession # CP006698.1). Vector Backbone:E. coli genome; Vector Types:Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin) See genbank file 2026-08-15 01:15:55 31
pFF19H
 
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RRID:Addgene_49783 HPH E. coli Ampicillin PMID:1369289 polylinker: SalI, XbaI, BamHI, SmaI, KpnI, SstI/SacI, NruI Backbone Marker:Messing Lab; Vector Backbone:pFF19, Addgene plasmid #49777; Vector Types:plant expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:02 0
pACM4-ACA
 
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RRID:Addgene_49809 ACP transacylase E. coli Chloramphenicol PMID:23361000 Backbone Size:3956; Vector Backbone:pACM4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol Silent site-directed mutagenesis to remove SalI site (GTCGAC to GTGGAC) 2026-08-15 01:16:02 0
Ptrc::galP_NS1
 
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1+ mentions
RRID:Addgene_101843 Ptrc promoter driving GalP transporter expression E. coli Nourseothricin (clonNat) PMID:28430105 Backbone Size:5300; Vector Backbone:pAM1303; Vector Types:Cloning vector; Bacterial Resistance:Nourseothricin (clonNat) 2026-08-15 01:00:22 1
pTR47m4-GFP
 
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RRID:Addgene_102436 Formaldehyde repressor protein (frmR) E. coli Ampicillin PMID:28921510 Vector Backbone:pTR47m4; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:00:24 0
pKSJ331
 
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1+ mentions
RRID:Addgene_103063 Colicin E1 and E1 immunity protein E. coli Ampicillin PMID:19029376 Insert was cloned from ~base pair 5015 to ~500 of the circular ColE1 plasmid, where numbering begins within the colicin E1 gene. The ColE1 sequences include both the promoter for colicin E1 and for E1 immunity protein, which is transcribed in the opposite direction from the colicin gene. The cloned DNA does not include the intact kil (lysis protein) gene. Backbone Marker:NEB; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:28 1
pKSJ376
 
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RRID:Addgene_103065 colicin E1 immunity protein E. coli Ampicillin PMID:27795317 The genes for colicin E1 with the R domain deletion and E1 immunity protein are transcribed from opposite strands of the vector. Colicin synthesis is under control of the vector inducible promoter; immunity protein synthesis is controlled by its own promoter. Backbone Marker:Novagen EMD/Millipore; Backbone Size:5627; Vector Backbone:pET52-b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:28 0
pMRBad-C-wtBlc
 
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RRID:Addgene_168257 C-fragment of wtBlc E. coli Kanamycin PMID:33210733 Backbone Size:4504; Vector Backbone:pMRBad; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:52 0
pRelA'
 
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RRID:Addgene_175595 Catalytic domain of E.coli relA gene. E. coli Kanamycin PMID:34990598 Backbone Marker:BglBrick; Vector Backbone:pBbS2k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Only the catalytic domain of the enzyme is used (C Terminal Domain [CTD] of WT RelA is removed). AGGAGGAAAAAA RBS sequence added before the start codon. 2026-08-15 01:10:52 0
pRelA*
 
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RRID:Addgene_175590 Catalytic domain of E.coli relA gene. E. coli Kanamycin PMID:34990598 Backbone Marker:BglBrick; Vector Backbone:pBbS2k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Only the catalytic domain of the enzyme is used (C Terminal Domain [CTD] of WT RelA is removed). AGGAGGAAAAAA RBS sequence added before the start codon. 2026-08-15 01:10:52 0
pFIP
 
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RRID:Addgene_17565 inversion switch E. coli Ampicillin PMID:16534780 Backbone Size:0; Vector Backbone:pBAD18; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:10:52 0
CAG.mCherry-eDHFR
 
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RRID:Addgene_176201 mCherry-eDHFR E. coli Ampicillin PMID:33904910 Please note that there are minor differences between the Addgene NGS result and the depositor's sequence. These differences do not alter plasmid function. Backbone Marker:Dr. Eugene Makeyev; Backbone Size:-1; Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:56 0
mU6-sgNeo1-hU6-sgNT
 
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RRID:Addgene_177211 sgNeo/sgNT1 E. coli Ampicillin Vector Backbone:pLL3.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:11:06 0
bU6-sgNeo1-mU6-sgNT-hU6-sgNeo2
 
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RRID:Addgene_177231 sgNeo1/sgNT1/sgNeo2 E. coli Ampicillin Vector Backbone:pLL3.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:11:06 0
AraC-pHG165c WT
 
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RRID:Addgene_170114 AraC E. coli Ampicillin PMID:34369028 Vector Backbone:pHG165c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:05 0

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