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Species: Gallus gallus
Genetic Insert: Vcl
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Chicken vinculin, full length(EcoRI fragment of p1005 subcloned into EcoRI-cut pEGFP-C1); A50I mutation (alanine 50 to isoleucine) introduced by QCPCR.
This mutant binds poorly (undetectably to talin in pull down assays; sensitivity of assay Kd= 10-20 uM).
Proper citation: RRID:Addgene_46267 Copy
Species: Gallus gallus
Genetic Insert: Vcl 1-851
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15728584
Comments: EYFP was PCR amplified to introduce NdeI site at both 5' and 3' ends. The NdeI flanked EYFP was inserted into NdeI site of pET15b/Vh1-851 (Addgene plasmid 46172), cloned between NdeI and XhoI. Plasmid contains a 12-residue spacer between the EYFP and vinculin domain.
Proper citation: RRID:Addgene_46264 Copy
Species: Gallus gallus
Genetic Insert: Vcl
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15728584
Comments: full length chicken vinculin (EcoRI fragment of p1005 subcloned into EcoRI-cut pEGFP-C1). ATG at bp 1390-1392. TAA at bp 4588-90. EGFP at N-terminus.
Proper citation: RRID:Addgene_46265 Copy
Species: Gallus gallus
Genetic Insert: Vcl 1-258
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15728584
Comments: EYFP was PCR amplified to introduce NdeI site at both 5' and 3' ends. The NdeI flanked EYFP was inserted into NdeI site of pET15b/Vh1-258 (Addgene plasmid 46173), where V1-258 was cloned between NdeI and XhoI. Plasmid contains a 12-residue spacer between the EYFP and vinculin domain.
Proper citation: RRID:Addgene_46263 Copy
Species: Gallus gallus
Genetic Insert: Vcl 884-1066
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: YFP subcloned into 15b/V884-1066 (Addgene plasmid 46176) via a PCR strategy which placed Nde sites at the ends of the YFP cDNA and added the spacer region in EGFPC1/V1-1066 at the Cterminus of YFP.
Proper citation: RRID:Addgene_46181 Copy
Species: Gallus gallus
Genetic Insert: Vcl 884-1066
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_46182 Copy
Species: Gallus gallus
Genetic Insert: Chicken ArcLight-S174
Vector Backbone Description: Backbone Size:5701; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24312287
Comments: The chicken voltage sensitive phosphatase (VSP) sequence used in this construct contains an E14K mutation, when compared to the reference sequence XP_417079.2 (the full plasmid sequence provided includes K14). This mutation could be polymorphism at the site or a PCR error. The depositing laboratory states that the plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_53567 Copy
Species: Gallus gallus
Genetic Insert: Chicken ArcLight-A173
Vector Backbone Description: Backbone Size:5701; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24312287
Comments: The chicken voltage sensitive phosphatase (VSP) sequence used in this construct contains an E14K mutation, when compared to the reference sequence XP_417079.2 (the full plasmid sequence provided includes K14). This mutation could be polymorphism at the site or a PCR error. The depositing laboratory states that the plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_53615 Copy
Species: Gallus gallus
Genetic Insert: CCPB1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mEmerald-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 487; Emission = 509. Localization data: Actin Capping Protein
Proper citation: RRID:Addgene_54027 Copy
Species: Gallus gallus
Genetic Insert: FAK
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mEmerald; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 487; Emission = 509. Localization data: Focal Adhesion Kinase
Proper citation: RRID:Addgene_54091 Copy
Species: Gallus gallus
Genetic Insert: LASP2
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mEmerald-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 487; Emission = 509. Localization data: Focal Adhesions
Proper citation: RRID:Addgene_54143 Copy
Species: Gallus gallus
Genetic Insert: C-Src
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mEmerald; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21378978
Comments: . Excitation = 487; Emission = 509. Localization data: Membrane/Vesicles
Proper citation: RRID:Addgene_54052 Copy
Species: Gallus gallus
Genetic Insert: Paxillin-KM
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mApple-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 568; Emission = 592
Proper citation: RRID:Addgene_54936 Copy
Species: Gallus gallus
Genetic Insert: FAK
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mApple; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 568; Emission = 592
Proper citation: RRID:Addgene_54898 Copy
Species: Gallus gallus
Genetic Insert: C-Src
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mApple; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 568; Emission = 592
Proper citation: RRID:Addgene_54868 Copy
Species: Gallus gallus
Genetic Insert: C-Src DN
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Kinase Dead K295R. Excitation = 587; Emission = 610
Proper citation: RRID:Addgene_55003 Copy
Species: Gallus gallus
Genetic Insert: C-Src
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19771335
Comments: . Excitation = 587; Emission = 610
Proper citation: RRID:Addgene_55002 Copy
Species: Gallus gallus
Genetic Insert: CCPB1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 587; Emission = 610
Proper citation: RRID:Addgene_55009 Copy
Species: Gallus gallus
Genetic Insert: CCPB1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 587; Emission = 610
Proper citation: RRID:Addgene_55010 Copy
Species: Gallus gallus
Genetic Insert: Paxillin KM
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 587; Emission = 610
Proper citation: RRID:Addgene_55115 Copy
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