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  • Organism:homo sapiens (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pU6-neo-TET
 
Resource Report
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1+ mentions
RRID:Addgene_51286 U6 Homo sapiens Ampicillin PMID:19390602 U6 is tagged with the self splicing intron neo cassette such that G418 resistance will be obtained only if retrotransposition occurs. Please note that the cassette is in the reverse orientation within the MCS relative to the full sequence--This has no functional consequence. Backbone Marker:Addgene plasmid 51283; Vector Backbone:pAluYa5-neo-TET; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:15 1
pBudORF2-CH
 
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1+ mentions
RRID:Addgene_51289 L1 ORF2 Homo sapiens Bleocin (Zeocin) PMID:21572950 This plasmid was created using the codon optimized L1RP as a source for the ORF2 coding sequence. Backbone Marker:Invitrogen; Backbone Size:4595; Vector Backbone:pBudCE4.1; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:16:15 2
pBS-L1PA1-CH-mneo
 
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1+ mentions
RRID:Addgene_51288 L1 Homo sapiens Ampicillin PMID:21572950 L1 is tagged with neoI indicator cassette containing an inverted neomycin resistance gene. The SV40 promoter (SV40p) drives transcription of the neomycin gene which is disrupted by an intron in the opposite orientation that renders it non-functional. The intron is only spliced out from tagged L1 transcripts generated by the CMV promoter. When retrotransposition of the spliced L1 RNA occurs, the new insert will contain a functional neo gene that is expressed from the SV40 promoter. The L1 open reading frames were codon optimized using Primo Optimum 3.4 (http://www.changbioscience.com/primo/pri​moo.html), which makes synonymous changes to optimal codons within the nucleotide sequence. A 63 bp inter-ORF with 92% sequence identity to L1RP was utilized for all constructs. Sequence changes to this region were generated to introduce restriction enzyme sites used for plasmid construction. The optimized nucleotide sequence is 74.9% identical to the sequence of L1RP. Backbone Marker:Stratagene; Vector Backbone:pBluescript II; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:12 4
L1-neo-TET
 
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1+ mentions
RRID:Addgene_51284 L1RE1 Homo sapiens Ampicillin PMID:19390602 L1 is tagged with the self splicing intron neo cassette such that G418 resistance will be obtained only if retrotransposition occurs. Vector Backbone:pBluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:12 6
pLenti.tie1.H2B.eYFP.W
 
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RRID:Addgene_51293 H2B-eYFP Homo sapiens Ampicillin PMID:20856866 Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2. Backbone Marker:Luigi Naldini; Backbone Size:6697; Vector Backbone:pRRLsin.cPPT.Tie1p.eGFP.wpre; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:16:12 0
pcDNA3-FLAG-Lin28BdelN70
 
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RRID:Addgene_51374 Lin28B Homo sapiens Ampicillin PMID:18951094 N-terminal deletion (70 amino acids) Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin N-terminal 70 amino acids deletion 2026-08-15 01:16:13 0
pcDNA3-pri-let-7a-1
 
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1+ mentions
RRID:Addgene_51377 pri-let-7a-1 Homo sapiens Ampicillin PMID:18951094 Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:13 2
pcDNA3-miR-29b-1~29a
 
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RRID:Addgene_51376 pri-miR-29b-1~29a Homo sapiens Ampicillin PMID:19079265 Point mutations: insertion (1115bp), deletion (A47, T666) (Unnecessary region for miRNA biogenesis) Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:13 0
pcDNA3-FLAG-Lin28B
 
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1+ mentions
RRID:Addgene_51373 Lin28B Homo sapiens Ampicillin PMID:18951094 Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:13 6
pcDNA3-FLAG-Lin28A-mCCHC
 
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1+ mentions
RRID:Addgene_51372 Lin28A Homo sapiens Ampicillin PMID:18951094 H147A, H169A Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin CCHC domain mutant (H147A, H169A) 2026-08-15 01:16:16 1
pcDNA3-pri-let-7b
 
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RRID:Addgene_51378 pri-let-7b Homo sapiens Ampicillin PMID:18951094 Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 0
KAP3-FL
 
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RRID:Addgene_51413 Kinesin-associated protein 3 Homo sapiens Kanamycin PMID:16262723 Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:16:13 0
pcDNA3-pri-let-7e
 
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1+ mentions
RRID:Addgene_51380 pri-let-7e Homo sapiens Ampicillin PMID:18951094 Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:13 1
pcDNA3-V5-DGCR8-mDRBD2
 
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RRID:Addgene_51386 DGCR8 Homo sapiens Ampicillin PMID:16963499 Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin DRBD2 mutant (A676K, S677K) 2026-08-15 01:16:13 0
pcDNA3-V5-DGCR8-mDRBD1&2
 
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RRID:Addgene_51385 DGCR8 Homo sapiens Ampicillin PMID:16963499 Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin DRBD1 & DRBD2 mutant (A568K, A569K, A676K, S677K) 2026-08-15 01:16:13 0
pcDNA3-V5-Lin28A
 
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RRID:Addgene_51387 Lin28A Homo sapiens Ampicillin PMID:19703396 Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:17 0
pcDNA3-pri-let-7g
 
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RRID:Addgene_51381 pri-let-7g Homo sapiens Ampicillin PMID:18951094 Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:17 0
pcDNA3-V5-DGCR8
 
Resource Report
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1+ mentions
RRID:Addgene_51383 DGCR8 Homo sapiens Ampicillin PMID:16963499 Addgene Quality Control sequencing has found some discrepancies in the full plasmid sequence in the V5 tag and linker region of the plasmid. The V5 tag and DGCR8 insert are not effected. Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:13 1
pGL3-CMV-Cdc42 3'UTR mutant (position 577-599 and 997-1019)
 
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RRID:Addgene_51390 NM_001039802.1 Homo sapiens Ampicillin PMID:19079265 miR-29 seed mutant (position: 578-584, 997-1003, TGCT->GTAG) Backbone Marker:Promega (modified to have 3' multi cloning sites); Vector Backbone:Cdc42 3'UTR; Vector Types:Luciferase; Bacterial Resistance:Ampicillin miR-29 binding site mutant (position 577-599, 997-1019) 2026-08-15 01:16:17 0
pGL3-CMV-p85a 3'UTR mutant
 
Resource Report
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RRID:Addgene_51393 p85alpha 3'UTR Homo sapiens Ampicillin PMID:19079265 miR-29 seed mutant (TGCT->GTAG) Backbone Marker:Promega (modified to have 3' multi cloning sites); Vector Backbone:pGL3-UC; Vector Types:Luciferase; Bacterial Resistance:Ampicillin miR-29 binding site mutant 2026-08-15 01:16:17 0

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