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Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5603; Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_114471 Copy
Species: Other
Genetic Insert: Hap2 + perDII
Vector Backbone Description: Vector Backbone:pIAGO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20189107
Comments: This plasmid encodes an AmphDI-PerDI hybrid glycosyltransferase and a PerDII perosamine synthase.
Proper citation: RRID:Addgene_114460 Copy
Species: Other
Genetic Insert: cPYP
Vector Backbone Description: Vector Backbone:PLL 7.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30203962
Comments: Plasmid lacks features necessary for lentiviral production, but should be unaffected for mammalian expression of tgRFP cPYP.
Proper citation: RRID:Addgene_114464 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5372; Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_114469 Copy
Species: Other
Genetic Insert: pegA
Vector Backbone Description: Vector Backbone:pIAGO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23563553
Proper citation: RRID:Addgene_114455 Copy
Species: Other
Genetic Insert: gCAG
Vector Backbone Description: Backbone Size:5257; Vector Backbone:pPN10; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27827362
Proper citation: RRID:Addgene_114384 Copy
Species: Other
Genetic Insert: gDM1d
Vector Backbone Description: Backbone Size:5257; Vector Backbone:pPN10; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27827362
Proper citation: RRID:Addgene_114387 Copy
Species: Other
Genetic Insert: dCas9-omega
Vector Backbone Description: Backbone Size:2574; Vector Backbone:p15a; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30272008
Proper citation: RRID:Addgene_114632 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Mammalian Expression, CRISPR, xenopus and zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24873830
Comments: Please see the Schier lab website http://labs.mcb.harvard.edu/schier/ for detailed protocols about target site selection, sgRNA production, stop codon cassette design, Cas9 protein purification, injection and downstream analysis.
This plasmid was used to prepare and inject Cas9 mRNA into zebrafish zygotes.
Proper citation: RRID:Addgene_47322 Copy
Species: Other
Genetic Insert: TRANSPORT INHIBITOR RESPONSE 1
Vector Backbone Description: Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23150568
Comments: Nishimura et al., 2009, Nature Methods
Please note that this version contains a nuclear export signal. A related construct without the NES can be found at: http://www.addgene.org/64945/
Proper citation: RRID:Addgene_47328 Copy
Species: Other
Genetic Insert: minCMV-dTomato_pA
Vector Backbone Description: Vector Backbone:pcrBluntII-topo; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47321 Copy
Species: Other
Genetic Insert: Cas9m2
Vector Backbone Description: Vector Backbone:pcDNA3.3_TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47314 Copy
Species: Other
Genetic Insert: wild type immunity region of lambda phage with tsr-venus-ub-cI
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:4225; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22751020
Proper citation: RRID:Addgene_47311 Copy
Species: Other
Genetic Insert: Cas9m2-VP64
Vector Backbone Description: Vector Backbone:pcDNA3.3_TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47317 Copy
Species: Other
Genetic Insert: Cas9m3-VP64
Vector Backbone Description: Vector Backbone:pcDNA3.3_TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47318 Copy
Species: Other
Genetic Insert: Cas9m3
Vector Backbone Description: Vector Backbone:pcDNA3.3_TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907171
Proper citation: RRID:Addgene_47315 Copy
Species: Other
Genetic Insert: Endophilin II
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2t; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Has A/G mutation resulting in K256E change
Proper citation: RRID:Addgene_47409 Copy
Species: Other
Genetic Insert: Endophilin
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2tk; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9341169
Proper citation: RRID:Addgene_47408 Copy
Species: Other
Genetic Insert: N-WASP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4735; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11584276
Comments: Original cDNA was a gift from Dr. Hiroaki Miki
Proper citation: RRID:Addgene_47406 Copy
Species: Other
Genetic Insert: CLCb
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4961; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15533941
Proper citation: RRID:Addgene_47404 Copy
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