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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pCI-EGFP-NR2a wt Resource Report Resource Website 1+ mentions |
RRID:Addgene_45445 | NR2A | Rattus norvegicus | Ampicillin | PMID:12160751 | Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | EGFP inserted after the predicted signal peptide cleavage site (after amino acid residue 21) | 2026-08-15 01:15:24 | 5 | |
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pCDNA3.1 AT1AR-TSTS/4A Resource Report Resource Website |
RRID:Addgene_45634 | AT1AR-TSTS/A | Rattus norvegicus | Ampicillin | PMID:15355986 | Mutant AT1A receptors were generated by mutagenesis PCR using pcDNA3.1-HA-AT1A receptor (provided by M. G. Caron, Duke University) as a template and a QuikChange multisite-directed mutagenesis kit (Stratagene) according to the manufacturer’s instructions. The primer for the TSTS/A receptor was: 5' p-CTCAAGCCTGTCTGCGAAAATGGCCGCTTGCTTACCGGCCTTC 3'. N4D mutation introduced to prevent glycosylation at this consensus site and steric hindrance of antibody binding. | Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3.1/Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Thr332, Ser335, Thr336, and Ser338 all changed to Alanine | 2026-08-15 01:15:25 | 0 |
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α1Ic-HE2-pcDNA3 Resource Report Resource Website |
RRID:Addgene_45808 | α1Ic | Rattus norvegicus | Ampicillin | PMID:12297319 | Backbone Marker:Perez-Reyes; Backbone Size:5400; Vector Backbone:pcDNA3-HE2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:26 | 0 | ||
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pCMV5 alpha 1A AR Resource Report Resource Website 1+ mentions |
RRID:Addgene_45760 | alpha 1A AR | Rattus norvegicus | Ampicillin | PMID:1970822 | Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:26 | 1 | ||
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pcDNA3.1 AT1R DRY-AAY Resource Report Resource Website 1+ mentions |
RRID:Addgene_45759 | AT1R DRY-AAY | Rattus norvegicus | Ampicillin | PMID:12746278 | The cDNA of the rat vascular smooth muscle AT1A receptor was donated by Dr. K. E. Bernstein (Emory University, Atlanta, GA). Mutations in the rat AT1A receptor were performed with the Mutagene kit(Bio-Rad Laboratories, Inc., Hercules, CA) N4D mutation is present to prevent glycosylation at this consensus site and steric hindrance of antibody binding. | Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | changed amino acids 125-126 from DR to AA | 2026-08-15 01:15:26 | 1 |
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pGEX-parkin W403A-C431S Resource Report Resource Website |
RRID:Addgene_45977 | Parkin W403A-C431S | Rattus norvegicus | Ampicillin | PMID:23661642 | Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Codon usage optimized for E.coli expression. Double mutation W403A-C431S | 2026-08-15 01:15:28 | 0 | |
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pGEX-parkin 219-465 Resource Report Resource Website |
RRID:Addgene_45972 | Parkin 219-465 | Rattus norvegicus | Ampicillin | PMID:23661642 | Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Codon usage optimized for E.coli expression. Deleted a.a. 1-219 | 2026-08-15 01:15:28 | 0 | |
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pGEX-parkin F146A Resource Report Resource Website |
RRID:Addgene_45973 | Parkin F146A | Rattus norvegicus | Ampicillin | PMID:23661642 | Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Codon usage optimized for E.coli expression. Changed Phe146 to alanine. | 2026-08-15 01:15:28 | 0 | |
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pGEX-parkin F463A Resource Report Resource Website |
RRID:Addgene_45975 | Parkin F463A | Rattus norvegicus | Ampicillin | PMID:23661642 | Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Codon usage optimized for E.coli expression. Changed Phe463 to alanine. | 2026-08-15 01:15:28 | 0 | |
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pGEX-parkin WT Resource Report Resource Website |
RRID:Addgene_45969 | Parkin | Rattus norvegicus | Ampicillin | PMID:23661642 | Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Codon usage optimized for E.coli expression | 2026-08-15 01:15:28 | 0 | |
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pT3-EFa1-HA-Jag1 Resource Report Resource Website |
RRID:Addgene_46051 | Jag1 | Rattus norvegicus | Ampicillin | PMID:23419361 | This plasmid is in the pT3-EF1a vector which contains loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid cannot not used with Cre for in vivo studies. | Backbone Size:7000; Vector Backbone:pT3-EF1a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:28 | 0 | |
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pcDNA3 βarr1 S412D Flag Resource Report Resource Website 1+ mentions |
RRID:Addgene_42196 | β-arrestin 1 S412D | Rattus norvegicus | Ampicillin | PMID:9924018 | Flag epitope-tagged truncation mutants of β-arrestin 1 were prepared by the polymerase chain reaction (PCR), incorporating an Eco RI site, minimal Kozak sequence (ACC), and initiator methionine codon into the 5' primer, and the Flag epitope sequence, stop codon, and an Xho I site into the 3' primer. PCR products were subcloned into pcDNA3. DNA sequences were confirmed by dideoxynucleotide sequencing. | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S412D | 2026-08-15 01:14:51 | 2 |
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ankG-mCherry Resource Report Resource Website 1+ mentions |
RRID:Addgene_42566 | ankyrin G | Rattus norvegicus | Kanamycin | PMID:21551097 | Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:55 | 5 | ||
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pRSET-RCaMP1h Resource Report Resource Website 1+ mentions |
RRID:Addgene_42874 | Red Fluorescent Calcium binding Protein | Rattus norvegicus | Ampicillin | PMID:23459413 | Gene was cloned using BaMHI and HindIII - But part of the vector backbone then becomes part of the protein (leader sequence containing histag) - using NdeI and HindIII will completely remove the open reading frame/gene. | Backbone Marker:Life Technologies (Invitrogen); Backbone Size:2753; Vector Backbone:pRSETA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:58 | 7 | |
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pXL-CAG-3XAP-NLG1 Resource Report Resource Website |
RRID:Addgene_43923 | 3XAP-NLG1 | Rattus norvegicus | Ampicillin | PMID:23457442 | Backbone Size:6390; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:11 | 0 | ||
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pcDNA3.1-Alpha-0 Dronpa Resource Report Resource Website |
RRID:Addgene_44193 | G protein alpha 0 | Rattus norvegicus | Ampicillin | PMID:17576765 | Dronpa was inserted between amino acid 92 and 93 using the KpnI and SpeI sites which were engineered between these sites. | Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Introduced KpnI and SpeI sites between 92nd and 93rd amino acids of alpha 0 | 2026-08-15 01:15:13 | 0 |
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pRc/CMV HA TrkB-T1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_39980 | TrkB.T1 | Rattus norvegicus | Ampicillin | PMID:9927421 | The T1 isoform of trkB was cloned from a pCMV-5 trkB.T1 construct using HindIII and XbaI to fuse it to the HA-tagged N-terminus of trkB in the vector pB-KS followed by subcloning into pRC/CMV AC7 with NheI and NotI. See Middlemas et al (Mol Cell Biol. 1991 Jan;11(1):143-53., https://www.ncbi.nlm.nih.gov/pubmed?term=1846020 ) to learn more about the TrkB.T1 variant. Please note that the endogenous signal sequence of TrkB-T1 was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to translated sequences for GenBank ID M55292.1 or NM_001163168.1, the TrkB-T1 protein sequence starts at amino acid #32 | Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRC/CMV AC7 vector, a derivative of the pRC/CMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | TrkB.T1 variant; endogenous signal sequence was replaced with BM40 and HA tag | 2026-08-15 01:14:32 | 2 |
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pRc/CMV HA TrkC Resource Report Resource Website |
RRID:Addgene_39979 | TrkC | Rattus norvegicus | Ampicillin | PMID:9927421 | cloned in the pRC/CMV AC7 vector, a derivative of the pRC/CMV vector (Invitrogen), which contains the BM40 signal peptide Please note that the endogenous signal sequence of TrkC was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to translated sequence for GenBank ID NM_001270656.1, the TrkC protein sequence starts at amino acid #32 | Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRC/CMV AC7 vector, a derivative of the pRC/CMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | endogenous signal sequence was replaced with BM40 and HA tag | 2026-08-15 01:14:32 | 0 |
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pRc/CMV HA TrkA Resource Report Resource Website 1+ mentions |
RRID:Addgene_39977 | TrkA | Rattus norvegicus | Ampicillin | PMID:9927421 | cloned in the pRC/CMV AC7 vector, a derivative of the pRC/CMV vector (Invitrogen), which contains the BM40 signal peptide Please note that the endogenous signal sequence of TrkA was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to GenBank ID NP_067600.1, the TrkA protein sequence starts at amino acid #30 | Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRC/CMV AC7 vector, a derivative of the pRC/CMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | endogenous signal sequence was replaced with BM40 and HA tag | 2026-08-15 01:14:32 | 1 |
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pCMV Myc rat SAPAP4 Resource Report Resource Website |
RRID:Addgene_40218 | SAPAP4 | Rattus norvegicus | Ampicillin | PMID:9115257 | Vector Backbone:pCMV5 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | N834D | 2026-08-15 01:14:34 | 0 |
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