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Species: Synthetic
Genetic Insert: SeqFosC
Vector Backbone Description: Backbone Size:7372; Vector Backbone:pAF1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24981864
Proper citation: RRID:Addgene_51428 Copy
Species: Synthetic
Genetic Insert: SeqFosA
Vector Backbone Description: Backbone Size:7485; Vector Backbone:pAF1-VP64; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24981864
Proper citation: RRID:Addgene_51430 Copy
Species: Synthetic
Genetic Insert: SeqFosC
Vector Backbone Description: Backbone Size:7705; Vector Backbone:pAF1-VP16; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24981864
Proper citation: RRID:Addgene_51432 Copy
Species: Synthetic
Genetic Insert: TALEN CCR5 Right (R18) Can C-term
Vector Backbone Description: Backbone Marker:Joung lab; Backbone Size:5055; Vector Backbone:JDS70; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24531420
Proper citation: RRID:Addgene_51439 Copy
Species: Synthetic
Genetic Insert: TALEN CCR5 Right (R18) Q3 C-term
Vector Backbone Description: Backbone Marker:Joung lab; Backbone Size:5055; Vector Backbone:JDS70; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24531420
Proper citation: RRID:Addgene_51441 Copy
Species: Synthetic
Genetic Insert: TALEN CCR5 Left (L18) Q3 C-term
Vector Backbone Description: Backbone Marker:Joung lab; Backbone Size:5055; Vector Backbone:JDS70; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24531420
Proper citation: RRID:Addgene_51440 Copy
Species: Synthetic
Genetic Insert: TALEN CCR5 Right (R18) N3 N-term
Vector Backbone Description: Backbone Marker:Joung lab; Backbone Size:5055; Vector Backbone:JDS70; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24531420
Proper citation: RRID:Addgene_51449 Copy
Species: Synthetic
Genetic Insert: TALEN CCR5 Left (L18) N3 N-term
Vector Backbone Description: Backbone Marker:Joung lab; Backbone Size:5055; Vector Backbone:JDS70; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24531420
Proper citation: RRID:Addgene_51448 Copy
Species: Synthetic
Genetic Insert: TALEN CCR5 Left (L18) N1 N-term
Vector Backbone Description: Backbone Marker:Joung lab; Backbone Size:5055; Vector Backbone:JDS70; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24531420
Proper citation: RRID:Addgene_51446 Copy
Species: Synthetic
Genetic Insert: Replacing the cpc operon with kanamycin resistance
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4835; Vector Backbone:pJ207; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25046143
Comments: Formighieri C, Melis A (2015) A phycocyanin·phellandrene synthase fusion enhances recombinant protein expression and β-phellandrene (monoterpene) hydrocarbons production in Synechocystis (cyanobacteria). Metab Eng 32:116–124. http://dx.doi.org/10.1016/j.ymben.2015.09.010
Proper citation: RRID:Addgene_51468 Copy
Species: Synthetic
Genetic Insert: Quasar2-mOrange-Chr64-GFP
Vector Backbone Description: Vector Backbone:lentivirus backbone; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_51656 Copy
Species: Synthetic
Genetic Insert: superfolder GFP
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23893733
Comments: The sfGFP in this plasmid contains Y148C and K214I mutations that do not affect the function of the plasmid.
Proper citation: RRID:Addgene_51559 Copy
Species: Synthetic
Genetic Insert: superfolder GFP
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_51562 Copy
Species: Synthetic
Genetic Insert: Codon-optimized Cas9 D10A
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24491566
Comments: This plasmid vector was constructed by mutation PCR of pCAG-T3-hCAS9-pA plasmid vector (Addgene plasmid 48625) using the primers (Forward primer, 5’-AGTACTCCATTGGGCTCGccATCGGCACAAAC, and Reverse primer, 5’-CGAGCCCAATGGAGTACTTCTTGTCGGCTGC).
For the in vitro synthesis of CAS9 D10A mRNAs, the vector was linearized by SphI and in-vitro transcribed using T3-RNA-polymerase (Promega) in the presence of m7G(5′)ppp(5′)G to synthesize capped RNA.
The Tbpl1 3′UTR 95 nucleotide polyA tail at the 3' end of the insert allows in vitro transcribed mRNA from this plasmid to be used directly for microinjection into animal embryos or oocytes without additional polyadenylation treatment.
The CAG promoter in this plasmid also allows it to be used as a CAS9-expression vector.
Proper citation: RRID:Addgene_51638 Copy
Species: Synthetic
Genetic Insert: EBFP2
Vector Backbone Description: Vector Backbone:pZDONOR 5-6; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25369267
Proper citation: RRID:Addgene_51790 Copy
Species: Synthetic
Genetic Insert: aDTX-lumitoxin
Vector Backbone Description: Backbone Size:5341; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24407101
Proper citation: RRID:Addgene_51686 Copy
Species: Synthetic
Genetic Insert: SpyLigase
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:6400; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24639550
Comments: SnoopLigase is the more recent peptide-peptide ligation technology from the Howarth lab and is now preferred over SpyLigase in terms of more general reaction conditions and higher coupling yield on a range of proteins.
pET28a-AviTag-SnoopLigase https://www.addgene.org/105626/
pET28a-HaloTag7-SnoopLigase https://www.addgene.org/105627/
SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. Jacob O. Fierer, Gianluca Veggiani and Mark Howarth
Transform plasmid into BL21 DE3 RIPL for expression.
Proper citation: RRID:Addgene_51722 Copy
Species: Synthetic
Genetic Insert: Optopatch2
Vector Backbone Description: Backbone Marker:Pavel Osten; Backbone Size:9236; Vector Backbone:FCK(1.3); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24952910
Proper citation: RRID:Addgene_51694 Copy
Species: Synthetic
Genetic Insert: Optopatch1
Vector Backbone Description: Backbone Marker:Pavel Osten; Backbone Size:9236; Vector Backbone:FCK(1.3); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24952910
Proper citation: RRID:Addgene_51695 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Zhang lab; Vector Backbone:lentiCRISPR (pXPR_001), Addgene plasmid #49535; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24336571
Comments: gRNA target sequence GGCCACAAGTTCAGCGTGTC
These six EGFP-targeting lentiCRISPRs have EGFP-targeting sgRNAs cloned into the lentiCRISPR backbone plasmid (Addgene plasmid 49535) and are used in Fig 1B of Shalem*, Sanjana* et al (2014). If you want to clone your own targeting sequences, please use the lentiCRISPR backbone plasmid (Addgene plasmid 49535), as the sgRNA Type IIs cloning site is not present in these plasmids.
Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check https://zlab.bio/ for the most up-to-date information.
Proper citation: RRID:Addgene_51762 Copy
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