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Species: Gallus gallus
Genetic Insert: mutated calmodulin gene
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2745; Vector Backbone:pEXP5-NT/TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23420322
Proper citation: RRID:Addgene_42568 Copy
Species: Gallus gallus
Genetic Insert: mutated calmodulin gene
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2745; Vector Backbone:pEXP5-NT/TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23420322
Proper citation: RRID:Addgene_42569 Copy
Species: Gallus gallus
Genetic Insert: Hen egg white lysozyme
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5641; Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16258828
Proper citation: RRID:Addgene_39233 Copy
Species: Gallus gallus
Genetic Insert: Focal Adhesion Kinase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5002; Vector Backbone:pcDNA-5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23393139
Comments: Discrepancies between QC and full sequence are of no functional consequence
Proper citation: RRID:Addgene_45047 Copy
Species: Gallus gallus
Genetic Insert: Hoxa6
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCAGGs; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23359544
Proper citation: RRID:Addgene_45552 Copy
Species: Gallus gallus
Genetic Insert: Hoxa5
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCAGGs; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23359544
Proper citation: RRID:Addgene_45551 Copy
Species: Gallus gallus
Genetic Insert: Hoxc8
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCAGGs; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23359544
Proper citation: RRID:Addgene_45555 Copy
Species: Gallus gallus
Genetic Insert: Hoxd10
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCAGGs; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18662545
Proper citation: RRID:Addgene_45721 Copy
Species: Gallus gallus
Genetic Insert: TN-XXL
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19160515
Proper citation: RRID:Addgene_45797 Copy
Species: Gallus gallus
Genetic Insert: Vcl 1-1066 + mCitrine
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pECFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17074767
Comments: Similar to FRET probe 1 (Addgene plasmid 46276) except ECFP changed to monomeric Citrine.
Proper citation: RRID:Addgene_46282 Copy
Species: Gallus gallus
Genetic Insert: ECFP + Vcl 1-419
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15883197
Comments: To make the control FRET probe pEYFP-C1/CFPV1-400, a HindIII site was engineered before the ATG site of pET15b/CFP-V1-851 and a KpnI site after codon 400 by PCR amplification with 5 -CAAGCTTCGatggtgagcaagggc-3 and 5 -GGTACCTCAtgcaactttccttgc-3 . The PCR product was introduced into TOPO pCRII and sequenced. The HindIII–KpnI fragment of CFP-vinculin1-400 was subcloned into pEYFP-C1 to generate the control plasmid pEYFP-CFPV1-400.
Tandem EYFP-ECFP- hooked to portion of vinculin that localizes to FA; maximum FRET.
Proper citation: RRID:Addgene_46277 Copy
Species: Gallus gallus
Genetic Insert: Vcl 1-1066
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEYFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15883197
Proper citation: RRID:Addgene_46278 Copy
Species: Gallus gallus
Genetic Insert: Vcl 1-1066 + EYFP
Vector Backbone Description: Backbone Marker:Craig lab; Vector Backbone:pECFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15883197
Comments: A 9-bp fragment encoding a NotI site was introduced by mutagenesis into pEGFP-C1/vinculin cDNA (chicken) immediately after the codon for aa 883 (Coutu and Craig, 1988) using
the Quick Change kit (Stratagene). The cDNA of EYFP (CLONTECH Laboratories, Inc.) minus the stop codon and flanked by NotI was inserted into NotI-digested vinculin.
Proper citation: RRID:Addgene_46276 Copy
Species: Gallus gallus
Genetic Insert: Vcl 1-851
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16608855
Comments: Nde-Xho fragment from pET15b/Vh1-851 (Addgene plasmid 46172) was subcloned into EGFP/V1-1066 (Addgene plasmid 46265) vector modified to contain unique Nde, Xho sites. Modifications were made by QuikChange PCR to facilitate cloning of cDNAs previously in the pET15b vector. The NdeI site present in the cytomegalovirus promoter region was removed (mutating adenine 234 to thymine), and
a new NdeI site was introduced into the multiple cloning site immediately before the vinculin start codon. Additionally, the 5-XhoI site was removed (mutating cytosine 1345 to adenine), and a 3-XhoI site was introduced 3' to the vinculin cDNA in lieu of a PstI site.
Proper citation: RRID:Addgene_46268 Copy
Species: Gallus gallus
Genetic Insert: TagRFP675
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3998; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23677204
Proper citation: RRID:Addgene_44276 Copy
Species: Gallus gallus
Genetic Insert: Flag-Sox9
Vector Backbone Description: Backbone Marker:Sean Megason and Andrew McMahon; Backbone Size:6240; Vector Backbone:pCIG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23382206
Proper citation: RRID:Addgene_44281 Copy
Species: Gallus gallus
Genetic Insert: V5-Snail2
Vector Backbone Description: Backbone Marker:Sean Megason and Andrew McMahon; Backbone Size:6240; Vector Backbone:pCIG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23382206
Proper citation: RRID:Addgene_44282 Copy
Species: Gallus gallus
Genetic Insert: SIINFEKL
Vector Backbone Description: Backbone Size:8300; Vector Backbone:PresentER; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30499773
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/22/267047 for bioRxiv preprint. Please note that the Addgene sequencing result identifies a V163A mutation within EGFP that does not affect fluorescence.
Proper citation: RRID:Addgene_102944 Copy
Species: Gallus gallus
Genetic Insert: GAPDH left arm
Vector Backbone Description: Backbone Size:4900; Vector Backbone:LSL-Cas9-Rosa26TV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29946437
Proper citation: RRID:Addgene_114009 Copy
Species: Gallus gallus
Genetic Insert: adenylate kinase isoenzyme 1 AK1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5443; Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30632751
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/18/420570 for bioRxiv preprint.
Proper citation: RRID:Addgene_118977 Copy
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