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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pCS2-Cas9
 
Resource Report
Resource Website
10+ mentions
RRID:Addgene_47322 Cas9 Other Ampicillin PMID:24873830 Please see the Schier lab website http://labs.mcb.harvard.edu/schier/ for detailed protocols about target site selection, sgRNA production, stop codon cassette design, Cas9 protein purification, injection and downstream analysis. This plasmid was used to prepare and inject Cas9 mRNA into zebrafish zygotes. Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Mammalian Expression, CRISPR, xenopus and zebrafish expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:40 15
pBabe TIR1-9myc
 
Resource Report
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10+ mentions
RRID:Addgene_47328 TRANSPORT INHIBITOR RESPONSE 1 Other Ampicillin PMID:23150568 Nishimura et al., 2009, Nature Methods Please note that this version contains a nuclear export signal. A related construct without the NES can be found at: http://www.addgene.org/64945/ Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:15:40 12
GFP-Rab35 WT
 
Resource Report
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1+ mentions
RRID:Addgene_47424 Rab35 Homo sapiens Kanamycin PMID:23264734 Backbone Marker:clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:15:41 8
pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)
 
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1+ mentions
RRID:Addgene_47542 Glutamate-gated chloride channels Caenorhabditis elegans Ampicillin PMID:23720773 Backbone Marker:Invitrogen; Backbone Size:2037; Vector Backbone:pcDNA3.1/V5-His TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin retention motif in ER (starting at R340) changed from RSR to AAA in the intracellular loop of Beta subunit; Y182F; monomerizing A206K in YFP 2026-08-15 01:15:42 1
pDD162 (Peft-3::Cas9 + Empty sgRNA)
 
Resource Report
Resource Website
100+ mentions
RRID:Addgene_47549 Cas9 Synthetic Ampicillin PMID:23995389 For more information on Goldstein Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Goldstein/ Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3 Backbone Size:2300; Vector Backbone:pCFJ601; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin Codon optimized and with synthetic introns for C. elegans 2026-08-15 01:15:42 164
GFP-Rab35 S22N inactive
 
Resource Report
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1+ mentions
RRID:Addgene_47426 Rab35 S22N Homo sapiens Kanamycin PMID:23264734 Backbone Marker:clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin S22N inactive 2026-08-15 01:15:41 4
pEN_TT MEK5-DD-mRFP1
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_47547 MEK5-DD Homo sapiens Gentamicin PMID:23332156 Backbone Marker:Addgene #25752; Backbone Size:4422; Vector Backbone:pEN_TTmiRc2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin S311D, T315D 2026-08-15 01:15:43 1
Tet-pLKO-puro-SOX2
 
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1+ mentions
RRID:Addgene_47540 SOX2 shRNA Synthetic Ampicillin PMID:22941189 Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin 2026-08-15 01:15:43 2
pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F (Opt α-GluClv2.0)
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_47387 glutamate-gated chloride Channels Caenorhabditis elegans Ampicillin PMID:23720773 Backbone Marker:Invitrogen; Backbone Size:5523; Vector Backbone:pcDNA3.1/V5-His TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Leu9 prime mutated to F (Phenylalanine in M3-M4 loop, L315F); monomerizing A206K in YFP 2026-08-15 01:15:41 1
Flag-Vac 14 Full Length
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_47418 Vac14 Homo sapiens Kanamycin PMID:17161399 Backbone Marker:Agilent; Backbone Size:4324; Vector Backbone:pCMV-Tag2B; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:15:42 3
pMSCVpuroATT
 
Resource Report
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1+ mentions
RRID:Addgene_47539 Chloramphenicol and Ampicillin PMID:23145123 Backbone Marker:Clontech; Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:15:42 1
Intersectin I SH3 A domain (human)
 
Resource Report
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1+ mentions
RRID:Addgene_47413 Intersectin SH3 domain A Homo sapiens Ampicillin PMID:9813051 Backbone Size:5000; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin only SH3 domain A 2026-08-15 01:15:41 2
MIGR1-cyclin E-AA
 
Resource Report
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1+ mentions
RRID:Addgene_47498 cyclin E1 Homo sapiens Ampicillin PMID:23873023 Human cyclin E1 (CCNE1) cDNA was mutated at N- and C-terminal Cdc4 phosphodegrons (T62A and T380A, see references) to disrupt interaction with Fbw7 ubiquitin ligase. cDNA is inserted into MIGR1 (MSCV-based retroviral vector), permitting transduction of hematopoietic cells. Cloned cDNA contains approximately 100 bp from CCNE1 3' UTR. GFP is co-expressed with cDNA via IRES, permitting enrichment for transduced cells using flow sorting. References: Koepp DM, Schaefer LK, Ye X, Keyomarsi K, Chu C, Harper JW et al (2001). Phosphorylation-dependent ubiquitination of cyclin E by the SCFFbw7 ubiquitin ligase. Science 294: 173-177. Strohmaier H, Spruck CH, Kaiser P, Won KA, Sangfelt O, Reed SI (2001). Human F-box protein hCdc4 targets cyclin E for proteolysis and is mutated in a breast cancer cell line. Nature 413: 316-322. Backbone Size:6521; Vector Backbone:MIGR1; Vector Types:Mammalian Expression, Retroviral, MSCV-IRES-GFP; Bacterial Resistance:Ampicillin threonines 62 and 380 to alanine (T62A T380A) 2026-08-15 01:15:42 1
Intersectin I SH3 D domain (human)
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_47416 Intersectin SH3 domain D Homo sapiens Ampicillin PMID:9813051 Backbone Size:5000; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin only SH3 domain D 2026-08-15 01:15:41 1
Intersectin I SH3 E domain (human)
 
Resource Report
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1+ mentions
RRID:Addgene_47417 ITSN1 intersectin 1 Homo sapiens Ampicillin PMID:9813051 Backbone Size:5000; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin only SH3 domain E 2026-08-15 01:15:41 1
Intersectin I SH3 B domain (human)
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_47414 Intersectin SH3 domain B Homo sapiens Ampicillin PMID:9813051 Backbone Size:5000; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin only SH3 domain B 2026-08-15 01:15:41 1
Endophilin II Full
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_47409 Endophilin II Other Ampicillin Has A/G mutation resulting in K256E change Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2t; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:41 1
GFP-N-WASP
 
Resource Report
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1+ mentions
RRID:Addgene_47406 N-WASP Other Kanamycin PMID:11584276 Original cDNA was a gift from Dr. Hiroaki Miki Backbone Marker:Clontech; Backbone Size:4735; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:15:41 3
pVC228 VEGF Site#3
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_47507 gRNA-VEGF site 3 Homo sapiens Ampicillin PMID:23792628 Target site: GGTGAGTGAGTGTGTGCGTG gRNA expression vector targeting human VEGF for use with JDS246 (Addgene plasmid# 43861--mammalian codon-optimized streptococcus pyogenes Cas9) Backbone Marker:Joung lab (Addgene plasmid #43860); Backbone Size:2278; Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:15:42 3
pEGFP GR
 
Resource Report
Resource Website
10+ mentions
RRID:Addgene_47504 Glucocorticoid receptor Homo sapiens Kanamycin Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:15:42 13

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