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Species: Saccharomyces cerevisiae
Genetic Insert: CRY2PHR-GAL4DBD
Vector Backbone Description: Backbone Size:8200; Vector Backbone:pSV605; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30340045
Proper citation: RRID:Addgene_112643 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SST2
Vector Backbone Description: Backbone Size:6600; Vector Backbone:pSV604; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30340045
Proper citation: RRID:Addgene_112642 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcs1 R54K
Vector Backbone Description: Vector Backbone:P415 Met25; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31331965
Proper citation: RRID:Addgene_112647 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcs1
Vector Backbone Description: Vector Backbone:P415 Met25; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31331965
Proper citation: RRID:Addgene_112646 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcs1 R54K AxxA
Vector Backbone Description: Vector Backbone:P415 Met25; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31331965
Proper citation: RRID:Addgene_112649 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eRF3
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34413231
Proper citation: RRID:Addgene_175418 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 3' homology for integration site XII-5
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176177 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dropout cassette containing toxic genes ccdB and TPK2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015).
Proper citation: RRID:Addgene_176179 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' homology for integration site XI-3
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176170 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' homology for integration site XI-5
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176172 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 3' homology for integration site XII-2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176175 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' homology for integration site XII-2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176174 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' homology for integration site XI-1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176166 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 3' homology for integration site XI-1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176167 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 3' homology for integration site XI-2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176169 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TPK2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015).
Proper citation: RRID:Addgene_176180 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ub CPS (8-16)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:parallel GST2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16462746
Comments: The vector backbone, parallel GST2, was made by replacing the polylinker of pGEX4T1 with the polylinker isolated from the baculovirus expression plasmid pFastBac (see PMID: 10024467).
Proper citation: RRID:Addgene_17631 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Osh4
Vector Backbone Description: Backbone Size:5000; Vector Backbone:modified pGEX-4T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16136145
Comments: DNA encoding Osh4 residues 2–434 was amplified by PCR and subcloned into the BamHI and XhoI sites of a pGEX-4T vector modified to contain a TEV protease recognition site.
Proper citation: RRID:Addgene_17632 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Vps23(1-385) – His/Vps28(1-242) – Vps37(1-213) – Mvb12(1-101)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pST39; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16615894
Comments: DNAs for Mvb12, Vps37, Vps28, and Vps23 were cloned into pST39.
Proper citation: RRID:Addgene_17634 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: His/Vps27(345-440) – Hse1(275-375)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pST39; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17543868
Proper citation: RRID:Addgene_17638 Copy
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