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Species: Saccharomyces cerevisiae
Genetic Insert: NAB3
Vector Backbone Description: Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22564898
Proper citation: RRID:Addgene_110534 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NAB3
Vector Backbone Description: Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22564898
Proper citation: RRID:Addgene_110532 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NAB3
Vector Backbone Description: Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29023495
Proper citation: RRID:Addgene_110537 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YBR139W
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29514932
Proper citation: RRID:Addgene_106470 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRC1
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29514932
Proper citation: RRID:Addgene_106471 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YBR139W(D415A)
Vector Backbone Description: Vector Backbone:pKP112; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29514932
Proper citation: RRID:Addgene_106468 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YBR139W(S219A)
Vector Backbone Description: Vector Backbone:pKP112; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29514932
Proper citation: RRID:Addgene_106466 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YBR139W
Vector Backbone Description: Vector Backbone:pRS416-PA; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29514932
Proper citation: RRID:Addgene_106461 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YBR139W(N163,242Q)
Vector Backbone Description: Vector Backbone:pRS416-PA; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29514932
Proper citation: RRID:Addgene_106462 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YBR139W(S219,D415,H474A)
Vector Backbone Description: Vector Backbone:pKP112; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29514932
Proper citation: RRID:Addgene_106469 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5886; Vector Backbone:pYES2.1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29326101
Comments: Contains the coding sequence for L-homoserine-O-acetyltransferase.
cerMET2 has A78T and G298E variants compared to the NCBI reference [NP_014122.1]. The plasmid has been used for complementation analyses and should function as described.
Proper citation: RRID:Addgene_107435 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5886; Vector Backbone:pYES2.1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29326101
Comments: contains the coding sequence for siroheme synthase
Proper citation: RRID:Addgene_107439 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5886; Vector Backbone:pYES2.1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29326101
Comments: contains the coding sequence for the beta subunit of sulfite reductase
Construct was not functional in complementation analysis, but this may be due to tags rather than mutations in the coding sequence. Use with caution.
Proper citation: RRID:Addgene_107437 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET17
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5886; Vector Backbone:pYES2.1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29326101
Comments: contains the coding sequence for homocysteine synthase
Proper citation: RRID:Addgene_107443 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET16
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5886; Vector Backbone:pYES2.1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29326101
Comments: contains the coding sequence for 3'-phosphoadenylsulfate reductase
Proper citation: RRID:Addgene_107442 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScPGM2 promoter-yEGFP
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29301066
Proper citation: RRID:Addgene_104502 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Tlg1
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Comments: Addgene's quality control identified G264D in URA3. The depositing lab states that this mutation should not affect selection.
Proper citation: RRID:Addgene_105261 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Arf1
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Proper citation: RRID:Addgene_105255 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Chc1 portion
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Proper citation: RRID:Addgene_105256 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Apl5
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Proper citation: RRID:Addgene_105253 Copy
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