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Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid # 48015); Vector Backbone:pAGM4723; Vector Types:Golden Gate assembly; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37278403
Comments: L2 empty backbone for golden gate assembly (Bpi) to diatoms.
This is a shorter backbone to improve transformation and cloning for systems that do not need the Agrobacterium T-DNA delivery.
The colonies are red due to the canthaxanthin operon. Successful insertion cut out the canthaxanthin operon and lead to white colonies.
Proper citation: RRID:Addgene_112207 Copy
Species: Synthetic
Genetic Insert: SpyCatcher-mi3
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30028591
Proper citation: RRID:Addgene_112255 Copy
Genetic Insert: E2F1RE (responsive element)
Vector Backbone Description: Vector Backbone:pGreenFire1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_112248 Copy
Species: Homo sapiens
Genetic Insert: IRS-1
Vector Backbone Description: Backbone Size:7583; Vector Backbone:pCMV histidinol; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10958681
Comments: pCMV human IRS-1 was cut with ApaLI and SalI. A 5' IRS-1 oligo at bp4746 (amino acid 1242) and 3'IRS-1 oligo with 3' IRS-1 sequence fused to the p21ras CAAX prenylation motif (CMSCKCVLS) were used for PCR and the insert was ligated into pCMV human IRS-1. Select cells expressing plasmid with approximately 5mM histidinol (Sigma), depending on the cell type.
Proper citation: RRID:Addgene_11238 Copy
Species: Homo sapiens
Genetic Insert: TFE3 homology arms flanking EGFP-IRES-Neo cassette
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Cloning source: K562. Use with plasmid pTFE3.1.0-gDNA.
Proper citation: RRID:Addgene_112377 Copy
Species: Homo sapiens
Genetic Insert: TFE3
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pTFE3-donor.
Proper citation: RRID:Addgene_112473 Copy
Species: Adenovirus
Genetic Insert: AdML-M3
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2500; Vector Backbone:pSP72; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12215496
Comments: Reed lab clone #388.
Proper citation: RRID:Addgene_11244 Copy
Species: Homo sapiens
Genetic Insert: CUX1
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pCUX1-donor.
Proper citation: RRID:Addgene_112434 Copy
Vector Backbone Description: Vector Backbone:pBAT4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8660525
Proper citation: RRID:Addgene_112592 Copy
Species: Synthetic
Genetic Insert: ProD gfpmut3 TT
Vector Backbone Description: Vector Backbone:pFPV25.1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29201859
Proper citation: RRID:Addgene_112518 Copy
Vector Backbone Description: Vector Backbone:pGEMEX1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8660525
Proper citation: RRID:Addgene_112580 Copy
Species: Homo sapiens
Genetic Insert: cathepsin B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16339146
Comments: See author's map for more information.
Proper citation: RRID:Addgene_11249 Copy
Vector Backbone Description: Vector Backbone:pBAT4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8660525
Proper citation: RRID:Addgene_112588 Copy
Vector Backbone Description: Vector Backbone:pBAT4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8660525
Proper citation: RRID:Addgene_112589 Copy
Vector Backbone Description: Backbone Size:5270; Vector Backbone:pRS410; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: pRS400 + pRS415 --> pRS410. Insert is KanMX (G418 resistance) from AatII-cut pRS400. Backbone is ApaLI-cut pRS415 (CEN plasmid).
Proper citation: RRID:Addgene_11258 Copy
Species: Homo sapiens
Genetic Insert: CDK4
Vector Backbone Description: Backbone Size:5558; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11884599
Comments: R24C allele of the CDK4 gene encodes a kinase that is insensitive to inhibition by p16INK4A and p15INK4B, the major negative regulators of CDK4.
Proper citation: RRID:Addgene_11254 Copy
Species: Synthetic
Genetic Insert: ChR2(H134R)
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pZac2.1; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31116972
Proper citation: RRID:Addgene_112496 Copy
Species: Homo sapiens
Genetic Insert: TPST2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12887918
Comments: See author's map for more information.
Proper citation: RRID:Addgene_11253 Copy
Vector Backbone Description: Backbone Size:5043; Vector Backbone:pRS418; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: p4339 + pRS410 --> pRS418. Insert is NatMX (clonNAT resistance) from EcoRI-cut p4339. Backbone is NcoI-cut pRS410 (CEN plasmid).
Proper citation: RRID:Addgene_11256 Copy
Vector Backbone Description: Backbone Size:4528; Vector Backbone:pRS408; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: pRS400 + pRS428 --> pRS408. Insert is NatMX (clonNAT resistance) from EcoRI-cut pRS428. Backbone is EcoRI-cut pRS400 (integrating plasmid).
Proper citation: RRID:Addgene_11255 Copy
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