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Species: Homo sapiens
Genetic Insert: SDCBP
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175484 Copy
Species: Homo sapiens
Genetic Insert: SDCBP
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175480 Copy
Species: Homo sapiens
Genetic Insert: SDCBP
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175482 Copy
Species: Homo sapiens
Genetic Insert: NF2
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175472 Copy
Species: Homo sapiens
Genetic Insert: PRDX1
Vector Backbone Description: Vector Backbone:pNIC-Bio3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2-pBirA cells for expression.
Please note: Plasmid is missing the GSGGGGSGGGGS linker sequence compared to the depositor's sequence. The missing extra linker between the full length coding sequence and Avitag does not affect function of the proteins.
Proper citation: RRID:Addgene_175473 Copy
Species: Homo sapiens
Genetic Insert: RAB5A
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175475 Copy
Species: Homo sapiens
Genetic Insert: RAB5A
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175476 Copy
Species: Homo sapiens
Genetic Insert: MSN
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37866628
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175470 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eRF3
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34413231
Proper citation: RRID:Addgene_175418 Copy
Species: Homo sapiens
Genetic Insert: CD44
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175448 Copy
Species: Homo sapiens
Genetic Insert: CD44
Vector Backbone Description: Vector Backbone:pNIC-CTH0; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175449 Copy
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Mouse Targeting, Cloning vector, to be used to deliver transgene into mice with dual heterologous Bxb1 attP sites; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35361849
Comments: We recommend this low-copy plasmid for the generation of donor DNA when the inserted transgene size is >15kb. For smaller transgene insertions, we recommend the high-copy plasmid p5154 (Addgene 175390).
Proper citation: RRID:Addgene_175391 Copy
Species: Homo sapiens
Genetic Insert: EZR
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175461 Copy
Species: Homo sapiens
Genetic Insert: MSN
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175465 Copy
Species: Homo sapiens
Genetic Insert: MSN
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37866628
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175468 Copy
Species: Homo sapiens
Genetic Insert: EPB41L3
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175458 Copy
Species: Mus musculus
Genetic Insert: Cd44
Vector Backbone Description: Vector Backbone:pNIC-CTH0; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175452 Copy
Species: Homo sapiens
Genetic Insert: CNN3
Vector Backbone Description: Vector Backbone:pSUMO-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning using SUMO-LIC5 and SUMO-LIC3 sites. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175456 Copy
Species: Homo sapiens
Genetic Insert: CAPN2 / CAPNS1
Vector Backbone Description: Vector Backbone:pET28a(+)-TEV; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Bicistronic for CAPN2 and CAPNS1. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175492 Copy
Species: Arabidopsis thaliana
Genetic Insert: ProGOXL4
Vector Backbone Description: Vector Backbone:pBI101.1; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34042158
Proper citation: RRID:Addgene_175560 Copy
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