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Species: Streptococcus pyogenes
Genetic Insert: SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101229 Copy
Species: Synthetic
Genetic Insert: hSpCas9-Cluster4(F491A/M495A/T496A/N497A)
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Proper citation: RRID:Addgene_101189 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cry2olig
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28041848
Comments: Our Cry2WT contains an additional 29 amino acids at the C-terminus of Cry2PHR. These additional residues are also retained in other constructs we used in the associated study.
Proper citation: RRID:Addgene_101222 Copy
Species: Homo sapiens
Genetic Insert: FUS(aa1-214)
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28041848
Comments: Our Cry2WT contains an additional 29 amino acids at the C-terminus of Cry2PHR. These additional residues are also retained in other constructs we used in the associated study.
Proper citation: RRID:Addgene_101224 Copy
Species: Homo sapiens
Genetic Insert: DDX4(1-236)
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28041848
Comments: Our Cry2WT contains an additional 29 amino acids at the C-terminus of Cry2PHR. These additional residues are also retained in other constructs we used in the associated study.
Proper citation: RRID:Addgene_101225 Copy
Species: Homo sapiens
Genetic Insert: MUC4
Vector Backbone Description: Vector Backbone:pSico; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28290446
Proper citation: RRID:Addgene_101152 Copy
Species: Homo sapiens
Genetic Insert: MUC4
Vector Backbone Description: Vector Backbone:pSico; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28290446
Proper citation: RRID:Addgene_101154 Copy
Species: Homo sapiens
Genetic Insert: Locus #2(tandem repeat)
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28290446
Proper citation: RRID:Addgene_101155 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101208 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9 variant N497A/R661A/Q695A/Q926A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101209 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HH-gRNA-HDV targetting SeILV6 in S. pastorianus
Vector Backbone Description: Backbone Size:5868; Vector Backbone:pUDP003; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29207996
Proper citation: RRID:Addgene_101166 Copy
Species: Other
Genetic Insert: MCP-YFP
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28290446
Proper citation: RRID:Addgene_101160 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101204 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9 variant Q926A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101206 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9 variant N497A/R661A/Q695A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101207 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HH-gRNA-HDV targetting SeILV6 in S. pastorianus
Vector Backbone Description: Backbone Marker:JM Daran; Backbone Size:10664; Vector Backbone:pUDP004; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29207996
Proper citation: RRID:Addgene_101167 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9 variant C80S/C574S/E60C/D273C
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101200 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
Vector Backbone Description: Backbone Marker:JM Daran; Backbone Size:10664; Vector Backbone:pUDP004; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29207996
Proper citation: RRID:Addgene_101168 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101203 Copy
Species: Homo sapiens
Genetic Insert: Cox8A
Vector Backbone Description: Backbone Size:6077; Vector Backbone:pCLIP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Originally cloned by Covalys. The CLIP-tag is an C-insert tag. Additional antibiotic resistance to Kanmycin
For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/
This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
Proper citation: RRID:Addgene_101130 Copy
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