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Species: Homo sapiens
Genetic Insert: BCL2 5'UTR - firefly luciferase
Vector Backbone Description: Backbone Marker:Stanley Lemon (PubMed ID 10611164); Vector Backbone:pRL-HL; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15123638
Comments: pBCL2-FL(pA) contains BCL2 5'UTR upstream of firefly luciferase. See plasmid map link above for more details.
This plasmid is one of several reporter constructs created to assess potential IRES activity of the BCL2 5'UTR.
Please note that Addgene's sequencing results differ from the full plasmid sequence provided by the depositing laboratory. These differences do not affect the function of the plasmid.
Proper citation: RRID:Addgene_42594 Copy
Species: Homo sapiens
Genetic Insert: hairpin - renilla luciferase - BCL2 5'UTR - firefly luciferase
Vector Backbone Description: Backbone Marker:Stanley Lemon (PubMed ID 10611164); Vector Backbone:pRL-HL; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15123638
Comments: phpRL-BCL2-FL(pA) includes a 143-bp stable RNA hairpin located upstream of renilla luciferase, BCL2 5'UTR and firefly luciferase. See plasmid map link above for more details.
This plasmid is one of several reporter constructs created to assess potential IRES activity of the BCL2 5'UTR. The hairpin in this construct inhibits translation of renilla luciferase and to a lesser extent translation of firefly luciferase.
Please note that Addgene's sequencing results differ from the full plasmid sequence provided by the depositing laboratory. These differences do not affect the function of the plasmid.
Proper citation: RRID:Addgene_42595 Copy
Species: Homo sapiens
Genetic Insert: Human H2B.1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5368; Vector Backbone:Pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_42630 Copy
Species: Homo sapiens
Genetic Insert: Human H3.1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5368; Vector Backbone:Pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_42631 Copy
Species: Homo sapiens
Genetic Insert: p21
Vector Backbone Description: Backbone Marker:Invivogen; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:23299246
Proper citation: RRID:Addgene_42623 Copy
Species: Homo sapiens
Genetic Insert: ZNF668
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8600; Vector Backbone:pLenti4/V5-Dest; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21852383
Proper citation: RRID:Addgene_42627 Copy
Species: Homo sapiens
Genetic Insert: Human H2AZ.1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5368; Vector Backbone:Pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_42629 Copy
Species: Homo sapiens
Genetic Insert: human MYCBP2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-3B; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16342205
Comments: Addgene QC NGS analysis identified sequence discrepancies relative to NM_015057. Please also see the associated publication for additional information on the construction of this plasmid.
Proper citation: RRID:Addgene_42570 Copy
Species: Homo sapiens
Genetic Insert: TBX5
Vector Backbone Description: Backbone Size:9335; Vector Backbone:pLX303; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23245941
Proper citation: RRID:Addgene_42563 Copy
Species: Homo sapiens
Genetic Insert: shTCF7L2
Vector Backbone Description: Backbone Marker:TRC; Backbone Size:8000; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23245941
Comments: TRCN0000262846
Proper citation: RRID:Addgene_42558 Copy
Species: Homo sapiens
Genetic Insert: EGFR ECD (1-644)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17283074
Proper citation: RRID:Addgene_42666 Copy
Species: Homo sapiens
Genetic Insert: EGFR ICD (645-1186)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17283074
Proper citation: RRID:Addgene_42667 Copy
Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16224021
Comments: Please note that there is an additional mutation in EZH2 ORF, Q3R. The mutation does not affect plasmid function.
Proper citation: RRID:Addgene_42664 Copy
Species: Homo sapiens
Genetic Insert: EGFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17283074
Proper citation: RRID:Addgene_42665 Copy
Species: Homo sapiens
Genetic Insert: HRAS V12
Vector Backbone Description: Backbone Marker:J Exp Med (1986) 164:1478; Backbone Size:3000; Vector Backbone:pcEXV-3; Vector Types:Eukaryotic; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_39528 Copy
Species: Homo sapiens
Genetic Insert: HRAS N17
Vector Backbone Description: Backbone Marker:J Exp Med (1986) 164:1478; Backbone Size:3000; Vector Backbone:pcEXV-3; Vector Types:Eukaryotic; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_39527 Copy
Species: Homo sapiens
Genetic Insert: HRAS V12
Vector Backbone Description: Backbone Marker:Addgene plasmid #1764; Vector Backbone:pBABE-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_39526 Copy
Species: Homo sapiens
Genetic Insert: HRAS V12
Vector Backbone Description: Backbone Marker:Addgene plasmid #1767; Vector Backbone:pBABE-neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_39525 Copy
Species: Homo sapiens
Genetic Insert: HRAS V12 E38
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9150145
Proper citation: RRID:Addgene_39520 Copy
Species: Homo sapiens
Genetic Insert: ARAP3(R307,8A)
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11804589
Proper citation: RRID:Addgene_39486 Copy
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