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Species: Homo sapiens
Genetic Insert: ZFP36L1 (TIS11B)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30449617
Proper citation: RRID:Addgene_121140 Copy
Species: Homo sapiens
Genetic Insert: CD274 with its own 3'UTR
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30449617
Proper citation: RRID:Addgene_121142 Copy
Species: Homo sapiens
Genetic Insert: 1-phosphatidylinositol 3-phosphate 5-kinase isoform 2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30612035
Proper citation: RRID:Addgene_121148 Copy
Species: Other
Genetic Insert: MVA Pathway (ERG12, ERG8, MVD1, IDI, MvaE, MvaS )
Vector Backbone Description: Vector Backbone:pCDF- Duet-1; Vector Types:Synthetic Biology; Bacterial Resistance:Streptomycin
Defining Citation: PMID:30103600
Proper citation: RRID:Addgene_121149 Copy
Species: Synthetic
Genetic Insert: mAID-mClover-BS
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31026591
Proper citation: RRID:Addgene_121193 Copy
Species: Other
Genetic Insert: BR32_00141031
Vector Backbone Description: Backbone Size:2626; Vector Backbone:pUC57-Kan; Vector Types:Golden Gate; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32460610
Proper citation: RRID:Addgene_121260 Copy
Species: Homo sapiens
Genetic Insert: TSC2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16636147
Comments: Compared to GenBank reference sequence NP_001107854.1, the TSC2 insert contains a S802R mutation.
Proper citation: RRID:Addgene_12132 Copy
Species: Mus musculus
Genetic Insert: Foxo1
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11696581
Comments: Plasmid contains K219R and L619P polymorphisms, which are not known to affect function.
Proper citation: RRID:Addgene_12142 Copy
Species: Mus musculus
Genetic Insert: p53
Vector Backbone Description: Backbone Size:5010; Vector Backbone:pLCR0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1761576
Comments: This plasmid, containing
the wild type murine p53 cDNA under the control of the previously
described BPV LCR-SV40 hybrid promoter (Spaholz et al., 1985),
was constructed as follows. Plasmid p407-1 (Spaholz et al., 1985) was
digested with EcoRI and HindIII, and the 5010-base pair fragment
containing the LCR-SV40 hybrid promoter was isolated and filled in
with Klenow.
After the ligation of BamHI linkers to the ends of the fragment, regenerating the terminal EcoRI site, the fragment was digested with
BamHI and ligated to the 2334-base pair BamHI fragment from the p53 cDNA expression vector pll-4 (Tan et al., 1986). This BamHI
fragment contained the entire wild type murine p53 coding region, 50 base pairs of 5'-untranslated sequence, 287 base pairs of 3'-untranslated
sequence, and a 3'-located SV40 mRNA splice site and polyA signal.
Proper citation: RRID:Addgene_12136 Copy
Species: Homo sapiens
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Vector Backbone:pLX317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30059005
Proper citation: RRID:Addgene_115439 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Erg11-iGFPx3
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30858194
Proper citation: RRID:Addgene_115422 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: vps10-104
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30858194
Comments: Please note sequence is from JK93da yeast strain rather than S288C
Proper citation: RRID:Addgene_115426 Copy
Species: Other
Genetic Insert: Renilla Luciferase
Vector Backbone Description: Backbone Marker:ThermoFisher; Vector Backbone:pTK; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15337849
Comments: clone number 122. BoxB repeat sequence: gggccctgaagaagggccc
Proper citation: RRID:Addgene_115365 Copy
Species: Other
Genetic Insert: Renilla Luciferase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCIneo; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16081698
Comments: clone number 137
Proper citation: RRID:Addgene_115366 Copy
Species: Other
Genetic Insert: HA
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCIneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15337849
Comments: clone number 211
Proper citation: RRID:Addgene_115360 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP104
Vector Backbone Description: Backbone Size:4895; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14978213
Proper citation: RRID:Addgene_1154 Copy
Species: bacteriophage P1
Genetic Insert: Phosphoglycerate kinase 1 promoter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2906; Vector Backbone:XhopKS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Full Gene Name: PGK-Cre-bpA: Phosphoglycerate kinase I promoter (PGK), the bacteriophage P1 recombinase Cre with a SV40 large T antigen nuclear localization signal (NLS-Cre), bovine growth hormone polyadenylation site (bpA). Use to express the Cre recombinase in mammalian cells. The vector backbone is a variant of pBluescriptKS- (Stratagene), in which the Xbal site was converted to an Xhol site (provided by Paul Hasty) (Soriano P., Cell 1997). The plasmid was generated by Kurt Fellenberg. He cloned a NLS-Cre sequence, as published by Hua Gu (Gu H, Zou YR, Rajewsky K. 1993. Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting. Cell. 73:1155-1164) and cloned it into the pPGK-neo-bpA (Soriano P, Montgomery C, Geske R, Bradley A. 1991 Targeted disruption of the c-src proto-oncogene leads to osteopetrosis in mice. Cell. Feb 22;64(4):693-702.), replacing the neomycin resistance gene (neo) with the NLS-Cre.
NOTE: There are some discrepancies between the Addgene quality control sequence and the sequence provided by the depositing lab, but these differences should not affect plasmid function.
Proper citation: RRID:Addgene_11543 Copy
Species: Synthetic
Genetic Insert: Green Fluorescent Protein
Vector Backbone Description: Vector Backbone:pRS406-ADH1/CYC1; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30804216
Comments: Please visit https://www.biorxiv.org/content/10.1101/345637v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_115565 Copy
Species: Homo sapiens
Genetic Insert: XIAP
Vector Backbone Description: Backbone Size:5353; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10797013
Proper citation: RRID:Addgene_11558 Copy
Species: Mus musculus
Genetic Insert: JNK KTR-mCherry
Vector Backbone Description: Backbone Marker:Allan Bradley (Wellcome Sanger Institute); Backbone Size:6777; Vector Backbone:pPB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30184500
Comments: See also: Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
Proper citation: RRID:Addgene_115493 Copy
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