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Species: Mus musculus
Genetic Insert: Wnt3A
Vector Backbone Description: Backbone Size:9220; Vector Backbone:pAdTrack-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15496414
Proper citation: RRID:Addgene_12518 Copy
Species: Mus musculus
Genetic Insert: MCK promoter
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescriptKS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9844629
Comments: A 6.5 kb XhoI fragment containing 3 kb of the MCK promoter and enhancer 1, untranslated exon 1, 3 kb of intron 1 including the enhancer region E2, and the first 16 bp of exon 2 was subcloned into the XhoI site of pBluescriptKS to result in pBSMCK. A PolyA sequence was added downstream (see author's map, MCK-polyA-2). Please note that the restriction sites shown between the MCK promoter and polyA region in the author's map are NOT unique sites.
Proper citation: RRID:Addgene_12528 Copy
Species: Mus musculus
Genetic Insert: CCAAT/Enhancer-binding Protein beta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The C/EBPbeta gene encodes 3 in-frame translational isoforms (LAP*, LAP, and LIP). LAP is the predominant activating isoform expressed in most cells, and this plasmid is designed to express the LAP isoform of the protein. The construct does not include the extra 21 codons at the N terminus that are present in LAP*. The complete insert (not just the ORF) includes a Kozak box sequence at the N terminus.
For more information on the C/EBPbeta isoforms, please see the original reference:
Descombes, P., and Schibler, U. A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA. Cell 67:569-579 (1991).
Proper citation: RRID:Addgene_12557 Copy
Species: Mus musculus
Genetic Insert: Kir2.1
Vector Backbone Description: Vector Backbone:pCALNL; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37290446
Comments: Please visit https://www.biorxiv.org/content/10.1101/625616v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_125578 Copy
Species: Mus musculus
Genetic Insert: Kir2.1
Vector Backbone Description: Vector Backbone:pCALNL; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37290446
Comments: Please visit https://www.biorxiv.org/content/10.1101/625616v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_125579 Copy
Species: Mus musculus
Genetic Insert: CCAAT enhancer binding protein gamma
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11980905
Proper citation: RRID:Addgene_12560 Copy
Species: Mus musculus
Genetic Insert: shRNA targeting Sik3
Vector Backbone Description: Backbone Size:7900; Vector Backbone:LEPG #111160; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31697837
Comments: Please visit https://www.biorxiv.org/content/10.1101/636969v1 for BioRxiv preprint
Proper citation: RRID:Addgene_125878 Copy
Species: Mus musculus
Genetic Insert: shRNA targeting Sik3
Vector Backbone Description: Backbone Size:7900; Vector Backbone:LEPG #111160; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31697837
Comments: Please visit https://www.biorxiv.org/content/10.1101/636969v1 for BioRxiv preprint
Proper citation: RRID:Addgene_125855 Copy
Species: Mus musculus
Genetic Insert: Zfp521
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pCMV Sport6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27107743
Comments: deletion of final six Zn fingers
Proper citation: RRID:Addgene_104190 Copy
Species: Mus musculus
Genetic Insert: Zfp521
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pCMV Sport6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27107743
Proper citation: RRID:Addgene_104189 Copy
Species: Mus musculus
Genetic Insert: Ran
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24908396
Proper citation: RRID:Addgene_104561 Copy
Species: Mus musculus
Genetic Insert: Mouse CL kappa
Vector Backbone Description: Backbone Marker:Invitrogen (Thermo Fisher); Backbone Size:10143; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30196504
Proper citation: RRID:Addgene_104579 Copy
Species: Mus musculus
Genetic Insert: Mouse IgG2c CH1/CH2/CH3 (mutated, no effector functions)
Vector Backbone Description: Backbone Marker:Invitrogen (Thermo Fisher); Backbone Size:10143; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30196504
Proper citation: RRID:Addgene_104581 Copy
Species: Mus musculus
Genetic Insert: calcium/calmodulin-dependent protein kinase II alpha
Vector Backbone Description: Backbone Size:2905; Vector Backbone:PX551; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29056297
Proper citation: RRID:Addgene_104589 Copy
Species: Mus musculus
Genetic Insert: gRNA targeting Tcf7l1
Vector Backbone Description: Backbone Marker:Yusa Lab; Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuroBFP-W; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29996108
Proper citation: RRID:Addgene_105015 Copy
Species: Mus musculus
Genetic Insert: Vps39
Vector Backbone Description: Backbone Marker:Yusa Lab; Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuroBFP-W; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29996108
Proper citation: RRID:Addgene_105019 Copy
Species: Mus musculus
Genetic Insert: Kcmf1
Vector Backbone Description: Backbone Marker:Yusa Lab; Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuroBFP-W; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29996108
Proper citation: RRID:Addgene_105017 Copy
Species: Mus musculus
Genetic Insert: Ndufa2
Vector Backbone Description: Backbone Marker:Yusa Lab; Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuroBFP-W; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29996108
Proper citation: RRID:Addgene_105030 Copy
Species: Mus musculus
Genetic Insert: Nelfb
Vector Backbone Description: Backbone Marker:Yusa Lab; Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuroBFP-W; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29996108
Proper citation: RRID:Addgene_105024 Copy
Species: Mus musculus
Genetic Insert: Nelfcd
Vector Backbone Description: Backbone Marker:Yusa Lab; Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuroBFP-W; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29996108
Proper citation: RRID:Addgene_105025 Copy
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