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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pUC57kan-T7-gRNA-U6 V2
 
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1+ mentions
RRID:Addgene_115520 sgRNA scaffolf-U6 promoter Other Kanamycin Vector Backbone:pUC57; Vector Types:CRISPR; Bacterial Resistance:Kanamycin 2026-08-15 01:02:34 3
pGPA1
 
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1+ mentions
RRID:Addgene_115476 Chloramphenicol and Gentamicin PMID:29162049 Backbone Size:11760; Vector Backbone:pZXL5; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Gentamicin 2026-08-15 01:02:34 1
pH-PABE-7-esgRNA
 
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1+ mentions
RRID:Addgene_115620 wtTadA-TadA7.10-nCas9-3*NLS Other Kanamycin PMID:29807545 Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin 2026-08-15 01:02:36 2
PABE-7
 
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RRID:Addgene_115628 wtTadA-TadA7.10-nCas9-3*NLS Other Ampicillin PMID:29807545 Vector Backbone:pJIT163; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:02:35 1
pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS
 
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RRID:Addgene_115694 NmeCas9 Neisseria meningitidis, strain 8013 Ampicillin PMID:30518407 Backbone Size:5693; Vector Backbone:pSimpleII; Vector Types:Mammalian Expression, Insect Expression, CRISPR; Bacterial Resistance:Ampicillin None 2026-08-15 01:02:35 2
pGM1192
 
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1+ mentions
RRID:Addgene_115683 mCherry, aac(3)IV, tsr Synthetic Apramycin Mobilizable, stable replicating expression vector for Streptomyces, strong constitutive SF14* promoter, codon optimized Strep-tagII-mCherry gene, tsr, aac(3)IV Vector Backbone:pSG5; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin mCherry codon optimized for Streptomyces 2026-08-15 01:02:37 1
pSico
 
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10+ mentions
RRID:Addgene_11578 Ampicillin PMID:15240889 pSico allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS ON shRNA expression. The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on http://web.mit.edu/jacks-lab/ (the Jacks Lab website) Backbone Size:7566; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin 2026-08-15 01:02:37 29
pMN19
 
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1+ mentions
RRID:Addgene_11572 CaMV 35S enhancers Spectinomycin PMID:10759496 Used to confirm activation-tagged genes. Contains multiple cloning sites adjacent to the same tetramerized CaMV 35S enhancers as in the activation-tagging vectors from this article. Kanamycin gene for plant selection on MS media. Plasmid selection in E. coli and in A. tumefaciens is spectinomycin (100 mg/l). The four enhancer repeats in the construct may be unstable in E. coli and Agrobacterium if stored at +4'C for extended time. Check by PCR with T7 (or M13-20) oligo and this one derived from the RB sequence: 5' acc cgc caa tat atc ctg 3'. This should give 1.4 kb band. Note that these oligos will not work in a transgenic plant, as the RB sequence is not transfered. Using this test, we found that after 1-2 weeks in a fridge the Agrobacterium strain loses on average one copy of a repeat, and after a month in a fridge there is only one copy left. It is a good idea to use a freshly streaked colony from a good -70'C glycerol stock for every infiltration. Backbone Marker:Hajdukiewicz et al 1994; Backbone Size:11073; Vector Backbone:pPZP212; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:36 1
pDSG360
 
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1+ mentions
RRID:Addgene_115601 regulated adhesin construct Kanamycin PMID:30033369 Vector Backbone:pSB3K3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:02:36 1
pDC191
 
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1+ mentions
RRID:Addgene_115792 Synaptobrevin2 Rattus norvegicus Ampicillin PMID:28772123 C103S is an additional mutation compared to NCBI reference- This will not affect plasmid function Backbone Marker:Novagen; Vector Backbone:pET31b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin S28C and C103S (please see comments below) 2026-08-15 01:02:36 1
pGMGUS
 
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1+ mentions
RRID:Addgene_115678 gusA, aac(3)IV, tsr Streptomyces Apramycin Thermosensitive replication (< 37°C) in Streptomyces, Detection of single/double cross over by X-gluc colour reaction , aac(3)IV Vector Backbone:pSG5; Vector Types:Gene knock out; Bacterial Resistance:Apramycin 2026-08-15 01:02:35 2
LV-Sox10MCS5-GFP
 
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1+ mentions
RRID:Addgene_115783 mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter Mus musculus Ampicillin PMID:23507034 Backbone Size:6378; Vector Backbone:SIN18; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:02:37 1
pKEW189-MBP-TEV-AcrVA1
 
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1+ mentions
RRID:Addgene_115660 AcrVA1 Moraxella bovoculi Ampicillin PMID:30190307 Backbone Marker:UC Berkeley; Backbone Size:5900; Vector Backbone:2CT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:02:35 1
PB-CAG-GFPd2
 
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1+ mentions
RRID:Addgene_115665 GFPd2 Synthetic Ampicillin PMID:30794383 Co-transfection with super piggybac transposase leads to stable integration in mammalian chromosomal DNA. Backbone Size:5551; Vector Backbone:pCAG-GFP; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:02:35 4
pCMV6-XL4 FLAG-NGRN-Fc
 
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1+ mentions
RRID:Addgene_115773 NGRN Homo sapiens Ampicillin Codon Optimized, FLAG-tag is followed by a NotI site and hIgG-tag is preceeded by a XbaI site. The reverse sequencing primer will sequence the CBLN1 insert only and not the hIgG-tag. Backbone Size:111; Vector Backbone:pCMV6-XL4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin none 2026-08-15 01:02:36 2
pCMV-Rev(NL4.3)
 
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1+ mentions
RRID:Addgene_115776 HIV-1 Rev protein (NL4.3) Other Kanamycin PMID:30735158 Also see Khoury et al. Retrovirology (2018) 15:36 https://doi.org/10.1186/s12977-018-0421-6 Vector Backbone:pHIS; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin None 2026-08-15 01:02:36 1
pKEW302-AcrVA4
 
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1+ mentions
RRID:Addgene_115656 AcrVA4 Moraxella bovoculi Ampicillin PMID:30190307 Backbone Marker:N/A; Backbone Size:2900; Vector Backbone:ColE1/AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:02:35 1
pSMAL-CellTag-V2
 
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1+ mentions
RRID:Addgene_115644 CellTag Barcode Synthetic Ampicillin PMID:30518857 The pSMAL backbone was a kind gift from John Dick: van Galen, P. et al. The unfolded protein response governs integrity of the haematopoietic stem-cell pool during stress. Nature 510, 268–72 (2014) Backbone Size:8427; Vector Backbone:pSMAL; Vector Types:Mammalian Expression, Lentiviral, Barcoding library; Bacterial Resistance:Ampicillin 2026-08-15 01:02:36 2
pCMV6-XL4 FLAG-CBLN1-Fc
 
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1+ mentions
RRID:Addgene_115765 CBLN1 Homo sapiens Ampicillin Codon Optimized, FLAG-tag is followed by a NotI site and hIgG-tag is preceeded by a XbaI site. The reverse sequencing primer will sequence the CBLN1 insert only and not the hIgG-tag. Backbone Size:111; Vector Backbone:pCMV6-XL4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin none 2026-08-15 01:02:37 1
pSMAL-CellTag-V3
 
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1+ mentions
RRID:Addgene_115645 CellTag Barcode Synthetic Ampicillin PMID:30518857 The pSMAL backbone was a kind gift from John Dick: van Galen, P. et al. The unfolded protein response governs integrity of the haematopoietic stem-cell pool during stress. Nature 510, 268–72 (2014) Backbone Size:8427; Vector Backbone:pSMAL; Vector Types:Mammalian Expression, Lentiviral, Barcoding library; Bacterial Resistance:Ampicillin 2026-08-15 01:02:35 2

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