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Species: Other
Genetic Insert: sgRNA scaffolf-U6 promoter
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_115520 Copy
Vector Backbone Description: Backbone Size:11760; Vector Backbone:pZXL5; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Gentamicin
Defining Citation: PMID:29162049
Proper citation: RRID:Addgene_115476 Copy
Species: Other
Genetic Insert: wtTadA-TadA7.10-nCas9-3*NLS
Vector Backbone Description: Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29807545
Proper citation: RRID:Addgene_115620 Copy
Species: Other
Genetic Insert: wtTadA-TadA7.10-nCas9-3*NLS
Vector Backbone Description: Vector Backbone:pJIT163; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29807545
Proper citation: RRID:Addgene_115628 Copy
Species: Neisseria meningitidis, strain 8013
Genetic Insert: NmeCas9
Vector Backbone Description: Backbone Size:5693; Vector Backbone:pSimpleII; Vector Types:Mammalian Expression, Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30518407
Proper citation: RRID:Addgene_115694 Copy
Species: Synthetic
Genetic Insert: mCherry, aac(3)IV, tsr
Vector Backbone Description: Vector Backbone:pSG5; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Comments: Mobilizable, stable replicating expression vector for Streptomyces, strong constitutive SF14* promoter, codon optimized Strep-tagII-mCherry gene, tsr, aac(3)IV
Proper citation: RRID:Addgene_115683 Copy
Vector Backbone Description: Backbone Size:7566; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15240889
Comments: pSico allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS ON shRNA expression.
The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on http://web.mit.edu/jacks-lab/ (the Jacks Lab website)
Proper citation: RRID:Addgene_11578 Copy
Genetic Insert: CaMV 35S enhancers
Vector Backbone Description: Backbone Marker:Hajdukiewicz et al 1994; Backbone Size:11073; Vector Backbone:pPZP212; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:10759496
Comments: Used to confirm activation-tagged genes. Contains multiple cloning sites adjacent to the same tetramerized CaMV 35S enhancers as in the activation-tagging vectors from this article.
Kanamycin gene for plant selection on MS media.
Plasmid selection in E. coli and in A. tumefaciens is spectinomycin (100 mg/l).
The four enhancer repeats in the construct may be unstable in E. coli and Agrobacterium if stored at +4'C for extended time. Check by PCR with T7 (or M13-20) oligo and this one derived from the RB sequence: 5' acc cgc caa tat atc ctg 3'. This should give 1.4 kb band. Note that these oligos will not work in a transgenic plant, as the RB sequence is not transfered. Using this test, we found that after 1-2 weeks in a fridge the Agrobacterium strain loses on average one copy of a repeat, and after a month in a fridge there is only one copy left. It is a good idea to use a freshly streaked colony from a good -70'C glycerol stock for every infiltration.
Proper citation: RRID:Addgene_11572 Copy
Genetic Insert: regulated adhesin construct
Vector Backbone Description: Vector Backbone:pSB3K3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30033369
Proper citation: RRID:Addgene_115601 Copy
Species: Rattus norvegicus
Genetic Insert: Synaptobrevin2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET31b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28772123
Comments: C103S is an additional mutation compared to NCBI reference- This will not affect plasmid function
Proper citation: RRID:Addgene_115792 Copy
Species: Streptomyces
Genetic Insert: gusA, aac(3)IV, tsr
Vector Backbone Description: Vector Backbone:pSG5; Vector Types:Gene knock out; Bacterial Resistance:Apramycin
Comments: Thermosensitive replication (< 37°C) in Streptomyces, Detection of single/double cross over by X-gluc colour reaction , aac(3)IV
Proper citation: RRID:Addgene_115678 Copy
Species: Mus musculus
Genetic Insert: mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
Vector Backbone Description: Backbone Size:6378; Vector Backbone:SIN18; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23507034
Proper citation: RRID:Addgene_115783 Copy
Species: Moraxella bovoculi
Genetic Insert: AcrVA1
Vector Backbone Description: Backbone Marker:UC Berkeley; Backbone Size:5900; Vector Backbone:2CT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190307
Proper citation: RRID:Addgene_115660 Copy
Species: Synthetic
Genetic Insert: GFPd2
Vector Backbone Description: Backbone Size:5551; Vector Backbone:pCAG-GFP; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30794383
Comments: Co-transfection with super piggybac transposase leads to stable integration in mammalian chromosomal DNA.
Proper citation: RRID:Addgene_115665 Copy
Species: Homo sapiens
Genetic Insert: NGRN
Vector Backbone Description: Backbone Size:111; Vector Backbone:pCMV6-XL4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Codon Optimized, FLAG-tag is followed by a NotI site and hIgG-tag is preceeded by a XbaI site. The reverse sequencing primer will sequence the CBLN1 insert only and not the hIgG-tag.
Proper citation: RRID:Addgene_115773 Copy
Species: Other
Genetic Insert: HIV-1 Rev protein (NL4.3)
Vector Backbone Description: Vector Backbone:pHIS; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30735158
Comments: Also see Khoury et al. Retrovirology (2018) 15:36
https://doi.org/10.1186/s12977-018-0421-6
Proper citation: RRID:Addgene_115776 Copy
Species: Moraxella bovoculi
Genetic Insert: AcrVA4
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:2900; Vector Backbone:ColE1/AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190307
Proper citation: RRID:Addgene_115656 Copy
Species: Synthetic
Genetic Insert: CellTag Barcode
Vector Backbone Description: Backbone Size:8427; Vector Backbone:pSMAL; Vector Types:Mammalian Expression, Lentiviral, Barcoding library; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30518857
Comments: The pSMAL backbone was a kind gift from John Dick: van Galen, P. et al. The unfolded protein response governs integrity of the haematopoietic stem-cell pool during stress. Nature 510, 268–72 (2014)
Proper citation: RRID:Addgene_115644 Copy
Species: Homo sapiens
Genetic Insert: CBLN1
Vector Backbone Description: Backbone Size:111; Vector Backbone:pCMV6-XL4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Codon Optimized, FLAG-tag is followed by a NotI site and hIgG-tag is preceeded by a XbaI site. The reverse sequencing primer will sequence the CBLN1 insert only and not the hIgG-tag.
Proper citation: RRID:Addgene_115765 Copy
Species: Synthetic
Genetic Insert: CellTag Barcode
Vector Backbone Description: Backbone Size:8427; Vector Backbone:pSMAL; Vector Types:Mammalian Expression, Lentiviral, Barcoding library; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30518857
Comments: The pSMAL backbone was a kind gift from John Dick: van Galen, P. et al. The unfolded protein response governs integrity of the haematopoietic stem-cell pool during stress. Nature 510, 268–72 (2014)
Proper citation: RRID:Addgene_115645 Copy
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