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Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 Aro2+
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232982 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 Aro3+
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232983 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 solvvol W+
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232977 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 S/ArotoL
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232979 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 R++
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232973 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 F108A
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232974 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 R++ W+
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232975 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 Proline
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232971 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 L113A
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene #14145); Vector Backbone:pAG415Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_232972 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Syf1
Vector Backbone Description: Vector Backbone:pRS414; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234114 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Syf1
Vector Backbone Description: Vector Backbone:pRS414; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234115 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cef1
Vector Backbone Description: Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234119 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Syf1
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234113 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cef1
Vector Backbone Description: Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234117 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MFA-IgKappa-FAPoptim-STE3
Vector Backbone Description: Vector Backbone:pRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The Ste3 promoter sequence (500 bprs upstream of the Ste3 ATG) was amplified from chromosomal DNA extracts using primers containing restriction enzyme adaptors for XhoI and XbaI. The pRS415-TEF1pr-Ste3-GFP plasmid was then digested with XhoI/XbaI to remove the TEF1 promoter and the Ste3 promoter sequences was ligated into its place.
Proper citation: RRID:Addgene_221112 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: STE3-GFP
Vector Backbone Description: Vector Backbone:pRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The pRS415-TEF1pr plasmid (Mumberg et al., 1995) was used to insert the Ste3 coding sequence fused at the C-terminus to GFP. The GFP was inserted into the plasmid first between the XhoI and SalI restriction enzyme sites and then Ste3 coding sequence (lacking the stop codon) was inserted into the SpeI and SmaI restriction enzyme sites.
Proper citation: RRID:Addgene_221113 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Fyv6
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234141 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Fyv6
Vector Backbone Description: Vector Backbone:pRS414; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234139 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Fyv6
Vector Backbone Description: Vector Backbone:pRS414; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234135 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Fyv6
Vector Backbone Description: Vector Backbone:pRS414; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234138 Copy
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