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Species: Mus musculus
Genetic Insert: p37
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30344098
Proper citation: RRID:Addgene_113504 Copy
Species: Mus musculus
Genetic Insert: p37
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30344098
Proper citation: RRID:Addgene_113503 Copy
Species: Mus musculus
Genetic Insert: p37
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30344098
Proper citation: RRID:Addgene_113502 Copy
Species: Mus musculus
Genetic Insert: PAR2
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29411438
Proper citation: RRID:Addgene_113609 Copy
Species: Mus musculus
Genetic Insert: I-Ab beta Derp1-117
Vector Backbone Description: Backbone Size:3818; Vector Backbone:pRMHa-3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29956152
Comments: This construct, when co-expressed with the corresponding alpha chain construct in Drosophila S2 cells and induced with copper sulfate, results in secreted expression of soluble peptide:MHCII complexes. The antigenic peptide sequence (residues 117-127 of Der p 1 allergen) is encoded as a fusion to the N-terminus of I-Ab beta, connected via a Gly-Ser linker. This fusion is encoded just downstream of the signal peptide, which should be cleaved during post-translational processing. Alternative antigenic peptides can be cloned in place of Der p 1 117-127 by exploiting the XmaI and SpeI restriction sites flanking this sequence.
Proper citation: RRID:Addgene_113639 Copy
Species: Mus musculus
Genetic Insert: I-Ab alpha BirA
Vector Backbone Description: Backbone Size:3808; Vector Backbone:pRMHa-3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29956152
Comments: This construct, when co-expressed with the corresponding beta chain construct in Drosophila S2 cells and induced with copper sulfate, results in secreted expression of soluble peptide:MHCII complexes.
Proper citation: RRID:Addgene_113638 Copy
Species: Mus musculus
Genetic Insert: miR-223
Vector Backbone Description: Backbone Size:6963; Vector Backbone:MDH1-PGK-GFP 2.0; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14657504
Proper citation: RRID:Addgene_11378 Copy
Species: Mus musculus
Genetic Insert: Cadm1
Vector Backbone Description: Backbone Marker:Jun-ichi Miyazaki; Vector Backbone:pCAG-GS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18003830
Proper citation: RRID:Addgene_115496 Copy
Species: Mus musculus
Genetic Insert: JNK KTR-mCherry
Vector Backbone Description: Backbone Marker:Allan Bradley (Wellcome Sanger Institute); Backbone Size:6777; Vector Backbone:pPB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30184500
Comments: See also: Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
Proper citation: RRID:Addgene_115493 Copy
Species: Mus musculus
Genetic Insert: SENP3
Vector Backbone Description: Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18948745
Proper citation: RRID:Addgene_115521 Copy
Species: Mus musculus
Genetic Insert: SENP3-C526A
Vector Backbone Description: Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18948745
Proper citation: RRID:Addgene_115522 Copy
Species: Mus musculus
Genetic Insert: Mouse Cdk4
Vector Backbone Description: Vector Backbone:pSRalpha-tkneo; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1423597
Proper citation: RRID:Addgene_115525 Copy
Species: Mus musculus
Genetic Insert: mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
Vector Backbone Description: Backbone Size:6378; Vector Backbone:SIN18; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23507034
Proper citation: RRID:Addgene_115783 Copy
Species: Mus musculus
Genetic Insert: Rag2
Vector Backbone Description: Vector Backbone:pLEXm; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21149691
Proper citation: RRID:Addgene_115650 Copy
Species: Mus musculus
Genetic Insert: Citrine-mTalin
Vector Backbone Description: Vector Backbone:pMpGWB105; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27703856
Proper citation: RRID:Addgene_100584 Copy
Species: Mus musculus
Genetic Insert: mTalin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5585; Vector Backbone:pDONR207; Vector Types:Repoter plasmid; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27703856
Proper citation: RRID:Addgene_100591 Copy
Species: Mus musculus
Genetic Insert: epn1
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28954824
Proper citation: RRID:Addgene_100733 Copy
Species: Mus musculus
Genetic Insert: mouse-Bnip3_deltaExon3
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26505960
Proper citation: RRID:Addgene_100793 Copy
Species: Mus musculus
Genetic Insert: Ctnnb1
Vector Backbone Description: Backbone Marker:Dave Turner Lab; Vector Backbone:pCS2MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28390865
Proper citation: RRID:Addgene_100883 Copy
Species: Mus musculus
Genetic Insert: Ctnnb1
Vector Backbone Description: Backbone Marker:Dave Turner Lab; Vector Backbone:pCS2MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28390865
Proper citation: RRID:Addgene_100884 Copy
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