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Species: Other
Genetic Insert: L7Ae
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2 DsRed-Express; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31765565
Comments: CDS of L7Ae was inserted between SalI and BamHI sites of pTAPmyc-T2A-tagRFP (ID: 140278).
Proper citation: RRID:Addgene_140263 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pGbait; Vector Types:Mouse Targeting; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32353257
Proper citation: RRID:Addgene_140283 Copy
Species: Other
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32353257
Proper citation: RRID:Addgene_140284 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:doi:10.1038/nchembio.1366; Backbone Size:14245; Vector Backbone:pKW20088; Vector Types:CRISPR, Synthetic Biology, Proof-of-concept test target of a in filamentous fungi.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32526136
Comments: To be cotransformed in strains expressing dSpCas9-VPR. This proof-of-concept vector is intended to serve as a positive control and aid the implementation of the CRISPRa vector set in other fungal species, facilitating initial testing and troubleshooting. The proof of concept activation is easily observable in Aspergillus nidulans with fluorescence microscopy when Cas9 is expressed from pCRI006. The mCherry mutation G174D does not appear to affect fluorescence.
Proper citation: RRID:Addgene_140205 Copy
Species: Other
Genetic Insert: Coat protein of bacteriphage MS2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2 DsRed-Express; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31765565
Comments: CDS of MS2CP was inserted between SalI and BamHI sites of pTAPmyc-T2A-tagRFP (ID: 140278).
Proper citation: RRID:Addgene_140259 Copy
Species: Other
Genetic Insert: T7-lacO-MobR-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140387 Copy
Species: Other
Genetic Insert: T7-lacO-CtcS-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140383 Copy
Species: Other
Genetic Insert: T7-lacO-CsoR-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140397 Copy
Species: Other
Genetic Insert: T7-lacO-SmtB-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140395 Copy
Species: Other
Genetic Insert: sgRNA for pAID-EF1a plasmid digestion
Vector Backbone Description: Backbone Size:8500; Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32941625
Comments: pX330 plasmid was obtained from Addgene (#42230).
Please visit https://www.biorxiv.org/content/10.1101/2020.01.20.912113v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_140609 Copy
Species: Other
Genetic Insert: LbCas12a
Vector Backbone Description: Backbone Marker:Fillip Port and Simon Bullock; Backbone Size:12400; Vector Backbone:act5c-Cas9; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32843348
Proper citation: RRID:Addgene_140620 Copy
Species: Other
Genetic Insert: LbCas12a
Vector Backbone Description: Backbone Marker:Johannes Bischof and Konrad Basler; Backbone Size:8500; Vector Backbone:pUASTattB; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32843348
Proper citation: RRID:Addgene_140621 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pBWB162; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32246719
Proper citation: RRID:Addgene_140636 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pBAV1k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32246719
Proper citation: RRID:Addgene_140634 Copy
Species: Other
Genetic Insert: CjCas9
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28220790
Proper citation: RRID:Addgene_137929 Copy
Species: Other
Genetic Insert: araC
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Backbone Size:3252; Vector Backbone:pSB3T5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:32251274
Proper citation: RRID:Addgene_138224 Copy
Species: Other
Genetic Insert: vir gene cluster
Vector Backbone Description: Vector Backbone:pVS1; Vector Types:Vir gene expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31558579
Proper citation: RRID:Addgene_138192 Copy
Species: Other
Genetic Insert: araC
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Backbone Size:3252; Vector Backbone:pSB3T5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:32251274
Proper citation: RRID:Addgene_138167 Copy
Species: Other
Genetic Insert: araC
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Backbone Size:3252; Vector Backbone:pSB3T5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:32251274
Proper citation: RRID:Addgene_138219 Copy
Species: Other
Genetic Insert: araC
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Backbone Size:3252; Vector Backbone:pSB3T5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:32251274
Proper citation: RRID:Addgene_138180 Copy
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