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Species: Homo sapiens
Genetic Insert: activating transcription factor 4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8102; Vector Backbone:pLVX-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30088945
Comments: This sensor leads to the expression of a mScarlet-I fusion protein in the nucleus upon induction of ER-stress. ER-stress kinetic through the PERK/ATF4 pathway can thus be monitored by recording the red fluorescent signal arising in the nucleus.
Designed by Adrien Nougarède.
Proper citation: RRID:Addgene_115969 Copy
Species: Synthetic
Genetic Insert: Lvl1 P2 With 3'UTR
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115956 Copy
Species: Homo sapiens
Genetic Insert: NSE promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14563701
Proper citation: RRID:Addgene_11606 Copy
Species: Homo sapiens
Genetic Insert: Insulin Growth Factor Binding Protein 5
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3-V5/His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11608 Copy
Species: Synthetic
Genetic Insert: Cit-tvmvs-DHFR
Vector Backbone Description: Vector Backbone:PB-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30237357
Proper citation: RRID:Addgene_116060 Copy
Species: Synthetic
Genetic Insert: TEVP
Vector Backbone Description: Vector Backbone:CMV-TO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30237357
Proper citation: RRID:Addgene_116062 Copy
Species: Homo sapiens
Genetic Insert: BRAF
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785
Proper citation: RRID:Addgene_116157 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP104
Vector Backbone Description: Backbone Size:5000; Vector Backbone:TMBS (CEN TRP1 MET3 promoter); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14978213
Proper citation: RRID:Addgene_1161 Copy
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:8500; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16550170
Comments: pLB is a modification of pLL3.7. Two genetic elements known to prevent epigenetic silencing were added. A fragment of one antirepressor element (#40) was cloned upstream of the mouse U6 promoter and a scaffold-attached region (SAR) was cloned downstream of GFP.
Please see author's map for more detailed information.
Note: A single base pair deletion at position 11 of the mouse U6 promoter in this plasmid does not impair the efficacy of this reagent.
There is also a base pair insertion upstream of the promoter. The depositing lab has no indication that it functionally impairs pLB.
Proper citation: RRID:Addgene_11619 Copy
Species: Homo sapiens
Genetic Insert: EGFR
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785
Proper citation: RRID:Addgene_116276 Copy
Species: Homo sapiens
Genetic Insert: merlin
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4950; Vector Backbone:pGEX2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10567424
Comments: The merlin insert in this plasmid contains A190V and G302E mutations compared to the closest match human Merlin reference sequence (NP_000259.1). The depositing laboratory states that these mutations should not affect merlin function, but cautions that it may be necessary to test merlin function if performing structure function experiments.
Proper citation: RRID:Addgene_11629 Copy
Species: Homo sapiens
Genetic Insert: CDKN2A
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785
Proper citation: RRID:Addgene_116222 Copy
Species: Homo sapiens
Genetic Insert: BRAF
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785
Proper citation: RRID:Addgene_116204 Copy
Species: Homo sapiens
Genetic Insert: moesin
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4970; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10567424
Proper citation: RRID:Addgene_11639 Copy
Species: Homo sapiens
Genetic Insert: NHE-RF
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4970; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10567424
Proper citation: RRID:Addgene_11636 Copy
Species: Homo sapiens
Genetic Insert: NHE-RF
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3/5'F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10567424
Proper citation: RRID:Addgene_11635 Copy
Species: Homo sapiens
Genetic Insert: moesin
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4970; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10567424
Proper citation: RRID:Addgene_11637 Copy
Species: Homo sapiens
Genetic Insert: ESR1
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785
Proper citation: RRID:Addgene_116372 Copy
Species: Homo sapiens
Genetic Insert: ESR1
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785
Proper citation: RRID:Addgene_116373 Copy
Species: Homo sapiens
Genetic Insert: ESR1
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785
Proper citation: RRID:Addgene_116374 Copy
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