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Species: Saccharomyces cerevisiae
Genetic Insert: mito-yHS1-M7A,H102A
Vector Backbone Description: Backbone Marker:M. Funk; Backbone Size:6900; Vector Backbone:p415GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32265272
Comments: The first-generation heme sensor, HS1, consists of a heme-binding domain, the His/Met coordinating 4-alpha-helical bundle hemoprotein cytochrome b562 (Cyt b562) (13), fused to a pair of fluorescent proteins, EGFP and Katushka 2 (mKATE2)
Proper citation: RRID:Addgene_159165 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
Vector Backbone Description: Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27939849
Comments: Yeast gene fragments were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genomic sequence.
Proper citation: RRID:Addgene_98299 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27939849
Comments: Yeast gene fragments were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genomic sequence.
Proper citation: RRID:Addgene_98298 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1
Defining Citation: PMID:28239415
Comments: Yeast gene fragments were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genomic sequence.
Proper citation: RRID:Addgene_98302 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TRPL3
Vector Backbone Description: Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29471044
Comments: Please set 1/1/2018 as the initial day available for public access. Yeast promoters and terminators were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genom
Proper citation: RRID:Addgene_98307 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1
Defining Citation: PMID:29471044
Comments: Please set 1/1/2018 as the initial day available for public access. Yeast gene fragments were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genomic seq
Proper citation: RRID:Addgene_98309 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Truncated ubiquitin cleavase, codon optimized
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:2200; Vector Backbone:pZE21; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29301968
Comments: Addgene NGS results found H571Y in the UBP1 translation compared to NP_010161.1. The depositing lab confirms that this mutation should not affect protein function
Proper citation: RRID:Addgene_98567 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Truncated ubiquitin cleavase, codon optimized
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:2200; Vector Backbone:pZE21; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29301968
Comments: Addgene NGS results found that the second tet operator in the tet promoter is missing; however the depositing laboratory confirmed that the plasmid functions normally
Proper citation: RRID:Addgene_98566 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ACT1 promoter
Vector Backbone Description: Backbone Size:4149; Vector Backbone:pFA6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29525204
Proper citation: RRID:Addgene_99049 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Tropomyosin
Vector Backbone Description: Vector Backbone:pJC20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17452625
Proper citation: RRID:Addgene_99355 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Swi1 N-rich region (AA1-327)
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:p416TEF1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20679490
Proper citation: RRID:Addgene_99553 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Swi1 NQ-rich region (AA1-536)
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:p415TEF1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20679490
Proper citation: RRID:Addgene_99554 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Swi1 N-rich region (AA1-327)
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:p413TEF1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28035761
Proper citation: RRID:Addgene_99557 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Swi1 NQ-rich region (AA1-536)
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:p413TEF1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28035761
Proper citation: RRID:Addgene_99556 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SMT3
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:4989; Vector Backbone:YEplac112; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19107412
Comments: The ORF is followed by the ADH1 transcription terminator. Please see secondary publication PMID: 22941627 for more information about this plasmid.
Proper citation: RRID:Addgene_99537 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: UBI3(1-76)
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:5241; Vector Backbone:YEplac195; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22941627
Comments: The ORF is followed by the ADH1 transcription terminator.
Proper citation: RRID:Addgene_99540 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: POL30
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:4293; Vector Backbone:YIplac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22941627
Comments: The ORF is followed by the ADH1 transcription terminator; digest with AflII, BbsI or XcmI for integration into LEU2. Please see secondary publication PMID: 19107412 for more information about this plasmid.
Proper citation: RRID:Addgene_99542 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: UBI3(1-76)
Vector Backbone Description: Backbone Marker:Sikorski & Hieter (1989) Genetics 122:19-27; Backbone Size:5797; Vector Backbone:pRS423; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22941627
Comments: The ORF is followed by the ADH1 transcription terminator.
Proper citation: RRID:Addgene_99541 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: POL30
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22941627
Comments: The ORF is followed by 1129 bp of the POL30 3' region; digest with StuI for integration into URA3 or with PmlI for integration upstream of endogenous POL30 (for exchange of alleles by 5FOA selection.) Please see secondary publication PMID: 19107412 for more information about this plasmid.
Proper citation: RRID:Addgene_99548 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: POL30
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22941627
Comments: The ORF is followed by 1129 bp of the POL30 3' region; digest with StuI for integration into URA3 or with PmlI for integration upstream of endogenous POL30 (for exchange of alleles by 5FOA selection.) Please see secondary publication PMID: 19107412 for more information about this plasmid.
Proper citation: RRID:Addgene_99547 Copy
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