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Species: Synthetic
Genetic Insert: sgPTPN14-4
Vector Backbone Description: Vector Backbone:LentiCRISPR v2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32581101
Proper citation: RRID:Addgene_163316 Copy
Species: Synthetic
Genetic Insert: antiGCN4scFV-VEEGFRtm-BFP (GCN4scFV-VB)
Vector Backbone Description: Backbone Size:8102; Vector Backbone:pLVX; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33025906
Proper citation: RRID:Addgene_163387 Copy
Species: Synthetic
Genetic Insert: LaG17-VEEGFRtm-BFP (LVB)
Vector Backbone Description: Backbone Size:8102; Vector Backbone:pLVX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33025906
Proper citation: RRID:Addgene_163381 Copy
Species: synthetic
Genetic Insert: lox-EM7p-Zeo-lox cassette flanked by Tn5 outer element
Vector Backbone Description: Backbone Marker:Epicentre; Backbone Size:2000; Vector Backbone:pMOD
Defining Citation: PMID:15784610
Comments: Note that Bleomycin and Zeocin are the same antibiotic.
Proper citation: RRID:Addgene_27182 Copy
Species: synthetic
Genetic Insert: MC1 promoter diphtheria toxin-A fragment
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2800; Vector Backbone:pBluescript II; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15784610
Comments: The MCS in the pBSDT-AII is extensibly modified by synthetic oligos to create multiple rare restriction sites
Proper citation: RRID:Addgene_27179 Copy
Species: Synthetic
Genetic Insert: TALE1
Vector Backbone Description: Backbone Size:9204; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21248753
Proper citation: RRID:Addgene_27969 Copy
Species: Synthetic
Genetic Insert: TAL-Backbone(NI)
Vector Backbone Description: Backbone Size:9204; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21248753
Proper citation: RRID:Addgene_27962 Copy
Species: Synthetic
Genetic Insert: Perceval (fluorescent ATP:ADP sensor)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2764; Vector Backbone:pRset B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19122669
Proper citation: RRID:Addgene_20336 Copy
Species: synthetic
Genetic Insert: opto-b2AR
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19295515
Comments: Please see http://www.optogenetics.org
for additional information.
Proper citation: RRID:Addgene_20948 Copy
Species: synthetic
Genetic Insert: opto-a1AR
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19295515
Comments: Please see http://www.optogenetics.org
for additional information.
Proper citation: RRID:Addgene_20947 Copy
Species: Synthetic
Genetic Insert: ECFP-IETD2x-Venus
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19053173
Comments: Initiator caspase reporter protein (IC-RP) carries tandem copies of IETD in its linker, a sequence that is efficiently cleaved by caspase-8, but poorly by caspases-3 and -7. IETD constitutes the site in procaspase-3 for initiator caspase cleavage, and IC-RP cleavage is therefore a good readout of procaspase-3 activation.
IC-RP was constructed by ligating Venus(YFP) between BamHI and EcoRI sites of pECFP-C1 and ligating linkers encoding cleavage sequences as BspEI-BamHI fragments between ECFP and Venus. Multiple serine and glycine residues flanking the cleavage sequences were added to increase linker flexibility and substrate accessibility. See https://www.ncbi.nlm.nih.gov/pubmed/18406323 for more information
Proper citation: RRID:Addgene_24536 Copy
Species: synthetic
Genetic Insert: Prb
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21458342
Proper citation: RRID:Addgene_24785 Copy
Species: synthetic
Genetic Insert: PdarC1
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pCTCON2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21458342
Proper citation: RRID:Addgene_24835 Copy
Species: Synthetic
Genetic Insert: Perceval (fluorescent ATP:ADP sensor)
Vector Backbone Description: Backbone Marker:British Biotech; Backbone Size:5076; Vector Backbone:pGW1-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19122669
Proper citation: RRID:Addgene_21737 Copy
Species: Synthetic
Genetic Insert: reverse tetracycline-regulated transactivator Advanced
Vector Backbone Description: Backbone Marker:unpublished; Backbone Size:7209; Vector Backbone:pMA2636rc; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19655272
Proper citation: RRID:Addgene_25434 Copy
Species: synthetic
Genetic Insert: mRFPmars
Vector Backbone Description: Backbone Marker:Martin Fraunholz; Backbone Size:6500; Vector Backbone:pCerulean; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20708040
Comments: Antibiotic selection: Ampicillin in E. coli, Chloramphenicol in S. aureus
Proper citation: RRID:Addgene_26252 Copy
Species: Synthetic
Genetic Insert: Codon optimized SpCas9-NG
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31501500
Comments: This plasmid vector was constructed by mutation PCR of pCAG-T3-hCAS9-pA plasmid vector (Addgene plasmid 48625) according to the amino acid sequences of SpCas9-NG shown in previous study (Nishimasu H et al., Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science, 2018).
Proper citation: RRID:Addgene_131465 Copy
Species: Synthetic
Genetic Insert: Codon optimized eSpCas9-NG
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31501500
Comments: This plasmid vector was constructed by mutation PCR of pCAG-T3-hCAS9-pA plasmid vector (Addgene plasmid 48625) according to the amino acid sequences of SpCas9-NG shown in previous study (Nishimasu H et al., Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science, 2018) with high-fidelity eSp variant shown in previous study (Slaymaker IM et al, Rationally engineered Cas9 nucleases with improved specificity. Science, 2016).
Proper citation: RRID:Addgene_131466 Copy
Species: Synthetic
Genetic Insert: Streptomyces codon optimized spCas9n (D10A)
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:31548381
Proper citation: RRID:Addgene_131464 Copy
Species: Synthetic
Genetic Insert: Codon optimized st1Cas9
Vector Backbone Description: Vector Backbone:pCAG-T3-hCAS9-pA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27318086
Proper citation: RRID:Addgene_131542 Copy
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