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Species: Homo sapiens
Genetic Insert: Ubiquitin C
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15728840
Comments: Please note that Addgene's sequencing results identified a V5 deletion in the ubiquitin sequence. This deletion was not intentional, but was present in the original source clone from which this plasmid was created (see associated publication for details).
Proper citation: RRID:Addgene_17602 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin C
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15728840
Proper citation: RRID:Addgene_17604 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin C
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15728840
Proper citation: RRID:Addgene_17605 Copy
Species: Homo sapiens
Genetic Insert: X-ray repair cross complementing 1
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34731617
Proper citation: RRID:Addgene_176062 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin C
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15728840
Proper citation: RRID:Addgene_17606 Copy
Species: Homo sapiens
Genetic Insert: ERBB2
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:9377; Vector Backbone:pLX304; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27416908
Proper citation: RRID:Addgene_175846 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176016 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176015 Copy
Species: Homo sapiens
Genetic Insert: IFN gamma promoter with 1st intron enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10640755
Comments: Young lab stock #D3314.
SalI should give 8.6kb + 0.6kb bands.
Proper citation: RRID:Addgene_17598 Copy
Species: Homo sapiens
Genetic Insert: IFN gamma promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10640755
Comments: Young lab stock #D3299.
SalI should give 8.5kb band
Proper citation: RRID:Addgene_17599 Copy
Species: Homo sapiens
Genetic Insert: Parkin
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11078524
Proper citation: RRID:Addgene_17612 Copy
Species: Homo sapiens
Genetic Insert: Parkin
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11078524
Proper citation: RRID:Addgene_17613 Copy
Species: Homo sapiens
Genetic Insert: EF-1a promoter
Vector Backbone Description: Backbone Marker:Dr. Didier Trono; Backbone Size:0; Vector Backbone:SIN LV pRLLhPGK.GFP Sin-18 (modified); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11781219
Comments: SIN lentiviral vector that expresses GFP controlled by the promoter of the housekeeping gene EF-1alpha.
We constructed EF.GFP by replacing the human PGK promoter (EcoRV to BamHI sites in the PGK.GFP vector) with human EF-1alpha promoter from plasmid pEF1/Myc-His-A (Invitrogen, Carlsbad, CA). The EF-1alpha promoter (nucleotide 373 to 1560 as numbered in GenBank accession J04616) contains the first exon, first intron, and part of the second exon before the coding sequence (starting at nucleotide 1582).
Recombinant LVs were generated using the 3-plasmid system by cotransfection of 293T cells through calcium phosphate precipitation. The ratio of a transducing vector, pMD.G, and pCMVDeltaR8.91 was fixed at 1.5:0.5:2 µg for 10^6 293T cells.
Proper citation: RRID:Addgene_17616 Copy
Species: Drosophila melanogaster
Genetic Insert: L596_g9608.t1
Vector Backbone Description: Backbone Size:8220; Vector Backbone:Addgene #104968; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34714893
Proper citation: RRID:Addgene_176078 Copy
Species: Drosophila melanogaster
Genetic Insert: L596_g25050.t1
Vector Backbone Description: Backbone Size:8220; Vector Backbone:Addgene #104968; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34714893
Proper citation: RRID:Addgene_176079 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin C
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15728840
Comments: The wild-type ubiquitin sequence in this plasmid is: MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG
Proper citation: RRID:Addgene_17608 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1 myc/his A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16269541
Comments: Compared to GenBank reference sequence NP_940980.3, the LRRK2 insert in this plasmid contains a M2397T mutation.
Proper citation: RRID:Addgene_17609 Copy
Species: Mus musculus
Genetic Insert: rhotekin
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37449837
Comments: Please visit https://doi.org/10.1101/2022.10.17.512253 for bioRxiv preprint.
Proper citation: RRID:Addgene_176108 Copy
Species: Homo sapiens
Genetic Insert: Paxillin
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34084168
Proper citation: RRID:Addgene_176107 Copy
Species: Mus musculus
Genetic Insert: rhotekin
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34357388
Proper citation: RRID:Addgene_176101 Copy
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