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Species: Saccharomyces cerevisiae
Genetic Insert: SUP35
Vector Backbone Description: Vector Backbone:pAG416-GAL-ccdb; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_181705 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Prp8
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234124 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Syf1
Vector Backbone Description: Vector Backbone:pRS414; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234126 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PrARG8-yeGFP
Vector Backbone Description: Backbone Marker:Irina Borodina Easy Clone 2.0 (Addgene #67296); Backbone Size:6049; Vector Backbone:pCfB2188; Vector Types:Yeast Expression, Cre/Lox, Integrative; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40172974
Comments: For further details refer to: EasyClone 2.0 Yeast Toolkit (Addgene Kit #1000000073 )
EasyClone 2.0: expanded toolkit of integrative vectors for stable gene expression in industrial Saccharomyces cerevisiae strains. Stovicek V, Borja GM, Forster J, Borodina I. J Ind Microbiol Biotechnol. 2015 Nov;42(11):1519-31. doi: 10.1007/s10295-015-1684-8. Epub 2015 Sep 16.
Proper citation: RRID:Addgene_216512 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC61-FAPoptim
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The coding sequence of SEC61 lacking the stop codon was PCR amplified with primers containing BamHI and SmaI restriction enzyme adaptors. The PCR product was then inserted into the pRS413-TEF1pr-NFAPoptim plasmid using standard cloning approaches.
Proper citation: RRID:Addgene_221126 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NAPstar3
Vector Backbone Description: Backbone Size:5542; Vector Backbone:p413 TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39702652
Proper citation: RRID:Addgene_232740 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FAPoptim
Vector Backbone Description: Vector Backbone:pFA6-NATMX4; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: This FAPoptim integration plasmid was constructed by subcloning the FAPoptim sequence into the pFA6a-NATMX4 plasmid described in (Goldstein and McCusker, 1999). The resulting plasmid can be used for C-terminal tagging of endogenous protiens using the PCR-based integration strategy described in (Longtine et al., 1998).
Proper citation: RRID:Addgene_221129 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ACT1-CUP1
Vector Backbone Description: Vector Backbone:pGAC24; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234131 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ACT1-CUP1
Vector Backbone Description: Vector Backbone:pGAC24; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234130 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ACT1-CUP1
Vector Backbone Description: Vector Backbone:pGAC24; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234129 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLPe recombinase
Vector Backbone Description: Vector Backbone:pEE083; Vector Types:Plant Expression, Recombinase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40111273
Proper citation: RRID:Addgene_234535 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Lifeact-3xmNeonGreen::TRP1
Vector Backbone Description: Vector Backbone:pRS304; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39024291
Proper citation: RRID:Addgene_227593 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPS21
Vector Backbone Description: Vector Backbone:pOPTH; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42207670
Proper citation: RRID:Addgene_235769 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: spt6-tsh2
Vector Backbone Description: Backbone Size:5429; Vector Backbone:pDEST15; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please note: This plasmid contains a V1480M mutation in the spt6 tsh2 domain. It is not known how this mutation affects plasmid function.
Proper citation: RRID:Addgene_227087 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YRR1
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37125648
Proper citation: RRID:Addgene_190585 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TEA1
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37125648
Proper citation: RRID:Addgene_190586 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU3
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37125648
Proper citation: RRID:Addgene_190582 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScHut1p-mTurquoise2-2xCrk_SH3
Vector Backbone Description: Backbone Marker:Internal; Vector Backbone:TMP138; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Comments: Additional inserts: 1) ScYnd1p(1-495)-ePTS1; 2) ScPex22(1-36)-mRuby2-SH3pep
Proper citation: RRID:Addgene_239510 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScYea4p-mTurquoise2-2xCrk_SH3
Vector Backbone Description: Backbone Marker:Internal; Vector Backbone:PSG747; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Comments: Additional inserts: 1) ScYnd1p(1-495)-ePTS1; 2) ScPex22(1-36)-mRuby2-SH3pep_dead
Proper citation: RRID:Addgene_239529 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScYea4p-mTurquoise2-2xCrk_SH3
Vector Backbone Description: Backbone Marker:Internal; Vector Backbone:PSG747; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Comments: Additional inserts: 1) ScYnd1p(1-495)-ePTS1; 2) ScPex22(1-36)-mRuby2-SH3pep
Proper citation: RRID:Addgene_239528 Copy
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