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Species: S pyogenes
Genetic Insert: SpCas9 EQR
Vector Backbone Description: Vector Backbone:px459 v2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29804665
Comments: Cas9 EQR sequence cloned from Addgene Plasmid #65772 cloned into px459 Addgene plasmid #62988
Proper citation: RRID:Addgene_101732 Copy
Species: S pyogenes
Genetic Insert: SpCas9 EQR
Vector Backbone Description: Vector Backbone:px458; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29804665
Comments: Cas9 EQR sequence cloned from Addgene Plasmid #65772 cloned into px458 Addgene plasmid #48138
Proper citation: RRID:Addgene_101731 Copy
Species: Rattus norvegicus
Genetic Insert: rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS
Vector Backbone Description: Backbone Marker:origene; Backbone Size:3405; Vector Backbone:CMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28740134
Proper citation: RRID:Addgene_101740 Copy
Genetic Insert: EGFP-miniAID
Vector Backbone Description: Vector Backbone:pcDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29804665
Comments: Please visit https://www.biorxiv.org/content/10.1101/182840v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_101714 Copy
Species: S pyogenes
Genetic Insert: SpCas9 VQR
Vector Backbone Description: Vector Backbone:px459 v2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29804665
Comments: Please visit https://www.biorxiv.org/content/10.1101/182840v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_101715 Copy
Species: Mus musculus
Genetic Insert: Pkr
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura; Vector Backbone:pMXs-IG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27633668
Proper citation: RRID:Addgene_101792 Copy
Genetic Insert: miniAID-EGFP
Vector Backbone Description: Vector Backbone:pcDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29804665
Comments: Please visit https://www.biorxiv.org/content/10.1101/182840v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_101713 Copy
Species: Mus musculus
Genetic Insert: Gcn2
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27633668
Proper citation: RRID:Addgene_101794 Copy
Species: Mus musculus
Genetic Insert: Activating transcription factor 4
Vector Backbone Description: Backbone Marker:Systems Biosciences Inc; Backbone Size:6592; Vector Backbone:pCDF1-MCS2-EF1-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101795 Copy
Species: Mus musculus
Genetic Insert: Fibroblast growth factor 21 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26487695
Proper citation: RRID:Addgene_101797 Copy
Species: S pyogenes
Genetic Insert: SpCas9 VRER
Vector Backbone Description: Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29804665
Comments: Cas9 VRER sequence cloned from Addgene Plasmid #65773 cloned into px330 Addgene plasmid #42230
Proper citation: RRID:Addgene_101729 Copy
Genetic Insert: S. pyogenes Cas9 codon optimized for expression in S. cerevisiae.
Vector Backbone Description: Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28886218
Proper citation: RRID:Addgene_101725 Copy
Species: S pyogenes
Genetic Insert: SpCas9 VRER
Vector Backbone Description: Vector Backbone:px458; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29804665
Comments: Cas9 VRER sequence cloned from Addgene Plasmid #65773 cloned into px458 Addgene plasmid #48138
Proper citation: RRID:Addgene_101728 Copy
Species: Cateneraria anguillulae
Genetic Insert: CatRhAC
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4568; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29799525
Proper citation: RRID:Addgene_101722 Copy
Species: Cateneraria anguillulae
Genetic Insert: CatRhGC
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4568; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29799525
Proper citation: RRID:Addgene_101720 Copy
Species: Homo sapiens
Genetic Insert: DYRK1A
Vector Backbone Description: Vector Backbone:pMH-SFB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38966995
Proper citation: RRID:Addgene_101770 Copy
Species: Homo sapiens
Genetic Insert: DYRK1B / MIRK
Vector Backbone Description: Vector Backbone:pMH-SFB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32611815
Proper citation: RRID:Addgene_101771 Copy
Species: Homo sapiens
Genetic Insert: BAF (BANF1)
Vector Backbone Description: Backbone Marker:Feng Zhang; Backbone Size:10370; Vector Backbone:Addgene plasmid # 52962; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28841419
Proper citation: RRID:Addgene_101774 Copy
Species: Homo sapiens
Genetic Insert: CETN2 Homology Arms with mTagRFPT-linker
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human CETN2 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_101781 Copy
Species: Homo sapiens
Genetic Insert: LAMP1 Homology Arms with linker-mEGFP
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human LAMP1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: EFGSTGSTGSTGADPPVAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_101782 Copy
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