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Genetic Insert: CAG promoters, GFP, tTR-KRAB, Tet-on
Vector Backbone Description: Backbone Size:12877; Vector Backbone:None; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16432520
Comments: Transgenes can be expressed from any RNA Pol II promoter as part of
bicistronic unit comprising the KRAB-based repressor; tetO sequences
are inserted into the vector LTR. Tet-on and Tet-off versions rely on
repressors that bind in the absence or the presence of doxycycline,
respectively. Addition of Pol III promoter-small hairpin RNA cassette
allows for drug-controllable RNA interference (Tet-on shRNA).
Cloning shRNA into pLVET, pLVCT, and pLVPT vectors: first clone
shRNA into pLVTHM downstream of the tetO-H1 region. Then cut pLVTHM
containing your shRNA with MscI-FspI and clone the insert containing
the 3'LTR to target plasmid opened with MscI-FspI. FspI cuts into
AmpR. This means for inverted clones AmpR will not be restored; after
selection you will be left with clones with the shRNA cassette and
functional AmpR.
pLVTHM and packaging plasmids for this system are also available at Addgene http://www.addgene.org/rnaitools Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab website http://tronolab.epfl.ch to see frequently asked
questions on cloning strategies and packaging.
Proper citation: RRID:Addgene_11643 Copy
Genetic Insert: hUbiquitin C, GFP, tTR-KRAB, no tetO
Vector Backbone Description: Backbone Size:11558; Vector Backbone:None; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16432520
Comments: pLVTHM and packaging plasmid for this system are also available at Addgene http://www.addgene.org/rnaitools Please visit Trono lab website http://tronolab.epfl.ch to see frequently asked questions on cloning strategies and packaging.
Proper citation: RRID:Addgene_11645 Copy
Genetic Insert: hEF-1alpha, GFP, tTR-KRAB, Tet-on
Vector Backbone Description: Backbone Size:12386; Vector Backbone:None; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16432520
Comments: Transgenes can be expressed from any RNA Pol II promoter as part of
bicistronic unit comprising the KRAB-based repressor; tetO sequences
are inserted into the vector LTR. Tet-on and Tet-off versions rely on
repressors that bind in the absence or the presence of doxycycline,
respectively. Addition of Pol III promoter-small hairpin RNA cassette
allows for drug-controllable RNA interference (Tet-on shRNA).
Cloning shRNA into pLVET, pLVCT, and pLVPT vectors: first clone
shRNA into pLVTHM downstream of the tetO-H1 region. Then cut pLVTHM
containing your shRNA with MscI-FspI and clone the insert containing
the 3'LTR to target plasmid opened with MscI-FspI. FspI cuts into
AmpR. This means for inverted clones AmpR will not be restored; after
selection you will be left with clones with the shRNA cassette and
functional AmpR.
pLVTHM and packaging plasmid for this system are also available at Addgene http://www.addgene.org/rnaitools Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit Trono lab website http://tronolab.epfl.ch to see frequently asked
questions on cloning strategies and packaging.
Proper citation: RRID:Addgene_11644 Copy
Species: Homo sapiens
Genetic Insert: MAP2K1
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785
Proper citation: RRID:Addgene_116428 Copy
Species: Homo sapiens
Genetic Insert: KRAS
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785
Proper citation: RRID:Addgene_116424 Copy
Species: Homo sapiens
Genetic Insert: mPGK, GDNF, rtTR-KRAB-2SM2, Tet-Off
Vector Backbone Description: Backbone Size:11516; Vector Backbone:pLVPT-rtTR-KRAB-2SM2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16432520
Comments: Transgenes can be expressed from any RNA Pol II promoter as part of
bicistronic unit comprising the KRAB-based repressor; tetO sequences
are inserted into the vector LTR. Tet-on and Tet-off versions rely on
repressors that bind in the absence or the presence of doxycycline,
respectively. Addition of Pol III promoter-small hairpin RNA cassette
allows for drug-controllable RNA interference (Tet-on shRNA).
Cloning shRNA into pLVET, pLVCT, and pLVPT vectors: first clone
shRNA into pLVTHM downstream of the tetO-H1 region. Then cut pLVTHM
containing your shRNA with MscI-FspI and clone the insert containing
the 3'LTR to target plasmid opened with MscI-FspI. FspI cuts into
AmpR. This means for inverted clones AmpR will not be restored; after
selection you will be left with clones with the shRNA cassette and
functional AmpR.
pLVTHM and packaging plasmid for this system are also available at Addgene http://www.addgene.org/rnaitools Please visit Trono lab website http://tronolab.epfl.ch to see frequently asked
questions on cloning strategies and packaging.
Proper citation: RRID:Addgene_11647 Copy
Species: Homo sapiens
Genetic Insert: PIK3CA
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785
Proper citation: RRID:Addgene_116479 Copy
Species: Homo sapiens
Genetic Insert: PIK3CA
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785
Proper citation: RRID:Addgene_116500 Copy
Species: Homo sapiens
Genetic Insert: PDGFRA
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785
Proper citation: RRID:Addgene_116453 Copy
Species: Homo sapiens
Genetic Insert: hUbiquitin C, GFP, tTR-KRAB, shRNA against GATA1, Tet-on
Vector Backbone Description: Backbone Size:11876; Vector Backbone:pLVUT-tTR-KRAB; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16432520
Comments: Transgenes can be expressed from any RNA Pol II promoter as part of
bicistronic unit comprising the KRAB-based repressor; tetO sequences
are inserted into the vector LTR. Tet-on and Tet-off versions rely on
repressors that bind in the absence or the presence of doxycycline,
respectively. Addition of Pol III promoter-small hairpin RNA cassette
allows for drug-controllable RNA interference (Tet-on shRNA).
Please visit Trono lab website http://tronolab.epfl.ch to see frequently asked
questions on cloning strategies and packaging.
Proper citation: RRID:Addgene_11650 Copy
Genetic Insert: hUbiquitin C, GFP, tTR-KRAB, Tet-on
Vector Backbone Description: Backbone Size:11480; Vector Backbone:None; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16432520
Comments: Transgenes can be expressed from any RNA Pol II promoter as part of bicistronic unit comprising the KRAB-based repressor; tetO sequences are inserted into the vector LTR. Tet-on and Tet-off versions rely on repressors that bind in the absence or the presence of doxycycline, respectively. Addition of Pol III promoter-small hairpin RNA cassette allows for drug-controllable RNA interference (Tet-on shRNA).
pLVTHM and packaging plasmid for this system are also available at Addgene http://www.addgene.org/rnaitools Please visit Trono lab website http://tronolab.epfl.ch to see frequently asked
questions on cloning strategies and packaging.
Note: There is another EcoRI site at 6235 that is not depicted in the author's map.
Proper citation: RRID:Addgene_11651 Copy
Species: Homo sapiens
Genetic Insert: PIK3CA
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785
Proper citation: RRID:Addgene_116457 Copy
Species: Homo sapiens
Genetic Insert: NRAS
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785
Proper citation: RRID:Addgene_116444 Copy
Species: Homo sapiens
Genetic Insert: PIK3CB
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785
Proper citation: RRID:Addgene_116553 Copy
Species: Homo sapiens
Genetic Insert: PIK3CB
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785
Proper citation: RRID:Addgene_116551 Copy
Species: Homo sapiens
Genetic Insert: PIK3CA
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533785
Proper citation: RRID:Addgene_116539 Copy
Species: Homo sapiens
Genetic Insert: hUbiquitin C, GFP, tTR-KRAB, shRNA against TP53, no tetO
Vector Backbone Description: Backbone Size:11579; Vector Backbone:pLVU-tTR-KRAB; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16432520
Comments: Please visit Trono lab website http://tronolab.epfl.ch to see frequently asked questions on cloning strategies and packaging.
Proper citation: RRID:Addgene_11654 Copy
Species: Homo sapiens
Genetic Insert: GFP, shRNA against TP53
Vector Backbone Description: Backbone Size:9931; Vector Backbone:None; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16432520
Comments: Please visit the Trono lab website: http://tronolab.epfl.ch to see frequently asked questions on cloning strategies and packaging.
shRNA sequence 5'-AGTAGATTACCACTGGAGTCTT
Proper citation: RRID:Addgene_11653 Copy
Vector Backbone Description: Backbone Size:8633; Vector Backbone:pPRIME-CMV-GFP-recipient; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16141338
Comments: This recipient plasmid contains pheS Gly294 between the I-SceI sites, which can be selected against using Cl-Phe.
Proper citation: RRID:Addgene_11657 Copy
Vector Backbone Description: Backbone Size:8719; Vector Backbone:pPRIME-CMV-Neo-recipient; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16141338
Comments: This recipient plasmid contains pheS Gly294 between the I-SceI sites, which can be selected against using Cl-Phe.
Proper citation: RRID:Addgene_11659 Copy
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