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Species: Saccharomyces cerevisiae
Genetic Insert: POL30
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22941627
Comments: The ORF is followed by 1129 bp of the POL30 3' region; digest with StuI for integration into URA3 or with PmlI for integration upstream of endogenous POL30 (for exchange of alleles by 5FOA selection.) Please see secondary publication PMID: 19107412 for more information about this plasmid.
Proper citation: RRID:Addgene_99549 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Kre33
Vector Backbone Description: Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29054886
Comments: Addgene NGS results found W737R in the Kre33 translation compared to the best-match NCBI reference sequence (EDN62683.1)
Proper citation: RRID:Addgene_99990 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rcl1
Vector Backbone Description: Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29054886
Comments: Compared to the best match NCBI reference sequence EIW07424.1, Addgene NGS results found A24T and R207S
Proper citation: RRID:Addgene_99992 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Brr2
Vector Backbone Description: Vector Backbone:pAS2-1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29054886
Proper citation: RRID:Addgene_99995 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sen1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29054886
Proper citation: RRID:Addgene_99994 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Pfa1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29054886
Proper citation: RRID:Addgene_99999 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mrt4
Vector Backbone Description: Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29054886
Proper citation: RRID:Addgene_99998 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Utp15
Vector Backbone Description: Vector Backbone:pAS2-1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29054886
Comments: Addgene NGS results detected the presence of an additional 29 amino acids at the C-terminus of the Utp15 translation compared to NP_013811.1
Proper citation: RRID:Addgene_99977 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Utp15
Vector Backbone Description: Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29054886
Comments: Addgene NGS results detected the presence of an additional 16 amino acids at the C-terminus of the Utp15 translation compared to NP_013811.1
Proper citation: RRID:Addgene_99978 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ckb1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29054886
Proper citation: RRID:Addgene_99981 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Utp16
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29054886
Proper citation: RRID:Addgene_99985 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Esf1
Vector Backbone Description: Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29054886
Proper citation: RRID:Addgene_99988 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Kre33
Vector Backbone Description: Vector Backbone:pAS2-1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29054886
Proper citation: RRID:Addgene_99989 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P-YPS1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: The part was cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176711 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P-SST2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: The part was cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176710 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-SPC42-Link-Neon(gfp)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179032 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-PDR16-Link-Neon(gfp)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179047 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: sopB
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35484249
Proper citation: RRID:Addgene_183677 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-SNF7-Link-Neon(gfp)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179041 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-PIL1-Link-Neon(gfp)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179039 Copy
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