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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pRK5-HA-Ubiquitin-K33 Resource Report Resource Website 10+ mentions |
RRID:Addgene_17607 | Ubiquitin C | Homo sapiens | Ampicillin | PMID:15728840 | Please note that Addgene's sequencing results identified P19S and E24D mutations in the ubiquitin sequence. These mutations were not intentional, but were present in the original source clone from which this plasmid was created (see associated publication for details). | Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | K33 only, other lysines mutated to arginines; P19S and E24D | 2026-08-15 01:10:54 | 30 |
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mNeonGreen-3xrGBD Resource Report Resource Website 1+ mentions |
RRID:Addgene_176091 | rhotekin | Mus musculus | Kanamycin | PMID:34357388 | Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:10:55 | 1 | ||
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H2A-mTurquoise-RAC1-G12V-deltaCAAX Resource Report Resource Website 1+ mentions |
RRID:Addgene_176095 | RAC1 | Homo sapiens | Kanamycin | PMID:34357388 | Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | G12V | 2026-08-15 01:10:55 | 1 | |
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dimericTomato-2xrGBD Resource Report Resource Website 1+ mentions |
RRID:Addgene_176098 | rhotekin | Mus musculus | Ampicillin | PMID:34357388 | Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:55 | 7 | ||
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PBSK HRbc Resource Report Resource Website 1+ mentions |
RRID:Addgene_1761 | human Rb | Homo sapiens | Ampicillin | Backbone Size:3000; Vector Backbone:pBSK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:55 | 1 | |||
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Lck-mTurquoise2-iLID Resource Report Resource Website 1+ mentions |
RRID:Addgene_176125 | iLID | Synthetic | Kanamycin | PMID:37449837 | Please visit https://doi.org/10.1101/2022.10.17.512253 for bioRxiv preprint. | Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:10:55 | 1 | |
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OA-1010Av2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_176082 | L596_g9608.t1 | Drosophila melanogaster | Ampicillin | PMID:34714893 | Backbone Size:8220; Vector Backbone:Addgene #104968; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:55 | 1 | ||
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pNLS-2xBFP(4C) Resource Report Resource Website 1+ mentions |
RRID:Addgene_176151 | NLS-2xBFP(4C) | Aequorea victoria | Ampicillin | PMID:35013558 | Backbone Marker:Invitrogen; Backbone Size:4404; Vector Backbone:pTrcHisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Compared with EGFP, the two BFP domains have the following mutations: L64F, Y66H, Q80R, V160A, S174C, and L230H | 2026-08-15 01:10:56 | 1 | |
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pLV-Hygro-Ef1A-Myc-POLB(PAMmut) Resource Report Resource Website 1+ mentions |
RRID:Addgene_176150 | PolB | Homo sapiens | Ampicillin | PMID:34731617 | Backbone Marker:VectorBuilder; Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | Mutation in the PAM site used by POLB gRNA1 | 2026-08-15 01:10:55 | 1 | |
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EF.CMV.RFP Resource Report Resource Website 10+ mentions |
RRID:Addgene_17619 | Ampicillin | PMID:12788657 | In a SIN LV containing the elongation factor 1alpha promoter, we included a second promoter from cytomegalovirus that drives expression of RFP as a reporter. Dual-promoter LVs can coexpress multiple transgenes efficiently in a single target cell. Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes. There is an a->g mutation in this plasmid that abolishes the HpaI site. A 10bp insertion in Addgene sequence corresponding to position 6893, 21bp insertion in Addgene sequence at position 7405, and 11bp deletion in Addgene sequence at position 7544 do not alter function of the plasmid or RFP expression. The uploaded author's map shows an example with GFP cloned downstream of the EF-1a promoter. The vector supplied by Addgene does not contain the GFP. | Backbone Size:8220; Vector Backbone:EF.CMV.RFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:56 | 16 | |||
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pAAV-hAdipoq-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_176215 | GFP | Other | Ampicillin | PMID:32184391 | Backbone Size:5050; Vector Backbone:pAAV-CBA-w; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:56 | 1 | ||
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DR.GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_17617 | HLA-DRa promoter | Homo sapiens | Ampicillin | PMID:11781219 | SIN lentiviral vector that expresses GFP controlled by the promoter of the human HLA-DRalpha gene, which is selectively expressed in antigen-presenting cells (APCs). We constructed DR.GFP by using the human (MHC II) HLA-DRalpha promoter (nucleotide 182 to 480, as numbered in GenBank accession X00274) to replace the PGK promoter (XhoI to BamHI sites in the PGK.GFP vector). This 300-base pair promoter sequence has been shown to confer selective expression of a transgene in MHC II+ cells. Recombinant LVs were generated using the 3-plasmid system by cotransfection of 293T cells through calcium phosphate precipitation. The ratio of a transducing vector, pMD.G, and pCMVDeltaR8.91 was fixed at 1.5:0.5:2 µg for 10^6 293T cells. | Backbone Marker:Dr. Didier Trono; Backbone Size:0; Vector Backbone:SIN LV pRLLhPGK.GFP Sin-18 (modified); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:56 | 1 | |
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EF.delmFasL.CMV.GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_17620 | delFasL | Mus musculus | Ampicillin | PMID:12788657 | The CMV promoter was inserted immediately upstream of GFP in the EF.GFP LV to make the intermediate LV, EF.V-CMV.GFP, with a unique EcoRV site available to insert a gene of interest under the control of the EF promoter. Noncleavable mouse delFasL cDNA was constructed using PCR cloning to delete the exon 2 region, which contains the metalloproteinase cleavage site. The delFasL gene was then cloned into the EcoRV site of EF.CMV.GFP to make the EF.delFasL-CMV.GFP LV. Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes. | Backbone Size:0; Vector Backbone:EF.CMV.GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | Noncleavable mouse delFasL . Deleted the exon 2 region, which contains the metalloproteinase cleavage site. | 2026-08-15 01:10:56 | 1 |
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EF.hICN1.CMV.GFP Resource Report Resource Website 10+ mentions |
RRID:Addgene_17623 | ICN1 | Homo sapiens | Ampicillin | PMID:16513761 | Addgene sequence shows that there is an A1902S mutation in Notch1 relative to NCBI sequences. This mutation is not known to affect function. The CMV promoter was inserted immediately upstream of GFP in the EF.GFP LV to make the intermediate LV, EF.V-CMV.GFP. hICN1 was cloned downstream of the EF-1a promoter. Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes. | Backbone Marker:Linzhao Cheng Lab; Backbone Size:8200; Vector Backbone:EF.v-CMV.GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:56 | 17 | |
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EF.hICN1.Ubc.GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_17626 | ICN1 | Homo sapiens | Ampicillin | PMID:16513761 | Addgene sequence shows that there is an A1902S mutation in Notch1 relative to NCBI sequences. This mutation is not known to affect function. hICN1 driven by the EF-1a promoter and a GFP reporter driven by the ubiquitin C (Ubc) promoter. Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes. | Backbone Size:11500; Vector Backbone:cFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:57 | 8 | |
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Duet011 Resource Report Resource Website 1+ mentions |
RRID:Addgene_17627 | UBC promoter - GFP - PGK promoter - Hygro | Ampicillin | PMID:17158240 | Lentiviral dual promoter/dual-gene expression vector is dubbed Duet (for Dual-gene, excisable transgene). | Backbone Size:0; Vector Backbone:Duet011; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:57 | 4 | ||
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pMVS223 Resource Report Resource Website 1+ mentions |
RRID:Addgene_176293 | Ampicillin | PMID:35120642 | Please visit https://www.biorxiv.org/content/10.1101/2020.10.28.359026v2 for bioRxiv preprint. | Backbone Size:5897; Vector Backbone:pMVS223; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:57 | 2 | |||
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pAB1678 CMV-HIVNES-GS-Cas13bt1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_176316 | human codon optimized Cas13bt1 | Atlantic deep subsurface metagenome | Ampicillin | PMID:34462587 | Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:57 | 1 | ||
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pGST1-Osh4 (2-434) Resource Report Resource Website 1+ mentions |
RRID:Addgene_17632 | Osh4 | Saccharomyces cerevisiae | Ampicillin | PMID:16136145 | DNA encoding Osh4 residues 2–434 was amplified by PCR and subcloned into the BamHI and XhoI sites of a pGEX-4T vector modified to contain a TEV protease recognition site. | Backbone Size:5000; Vector Backbone:modified pGEX-4T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:57 | 1 | |
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pGFP-Ub-VPS13D Resource Report Resource Website 1+ mentions |
RRID:Addgene_176462 | VPS13D | Homo sapiens | Kanamycin | PMID:33891012 | GFP-Ub-VPS13D will be translated into one fusion protein. However, endogenous DUBs will cleave it into GFP-Ub and VPS13D fragments. GFP-Ub hence acts as a fluorescent reporter for VPS13D expression. | Backbone Marker:Clontech; Backbone Size:3679; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:10:59 | 1 |
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