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Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-Neon(gfp)-Link-SED5
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179036 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-Neon(gfp)-Link-SCS2tmh
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179035 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-preCOX4-Link-Scarlet(rfp)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179070 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-PEX25-Link-Scarlet(rfp)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179069 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-Scarlet(rfp)-Link-VAM3
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179064 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-ERG6-Link-Scarlet(rfp)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179067 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-PIL1-Link-Scarlet(rfp)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179060 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-Scarlet(rfp)-Link-SFT2
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179058 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-SRM1-Link-Scarlet(rfp)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179052 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-RPC19-Link-Scarlet(rfp)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179051 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-Scarlet(rfp)-Link-SED5
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179057 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-Scarlet(rfp)-Link-TUB1
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179054 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-ARC35-Link-Scarlet(rfp)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179055 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TDH3p-Neon(gfp)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179077 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AMN1 (left homology arm) - KanMX - AMN1 (right homology arm)
Vector Backbone Description: Backbone Marker:Harvard Medical School; Backbone Size:3607; Vector Backbone:pBY011; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35361876
Proper citation: RRID:Addgene_183099 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BRR2
Vector Backbone Description: Backbone Size:4957; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27072132
Comments: Ledoux and Guthrie, JBC, 2017
Proper citation: RRID:Addgene_90257 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BRR2
Vector Backbone Description: Backbone Size:4957; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27072132
Comments: Ledoux and Guthrie, JBC, 2017
Proper citation: RRID:Addgene_90256 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pZ-SIC1
Vector Backbone Description: Backbone Size:6559; Vector Backbone:pNH604; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27930885
Proper citation: RRID:Addgene_90408 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SAD1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24681967
Comments: Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus.
Proper citation: RRID:Addgene_90305 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SAD1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24681967
Comments: Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus.
Proper citation: RRID:Addgene_90304 Copy
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