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Species: Synthetic
Genetic Insert: gRNA scaffold
Vector Backbone Description: Vector Backbone:pCambia; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: Modified from the pHEE401E T-DNA vector described by Wang &al. (2015) Genome Biol 16: 144, doi:10.1186/s13059-015-0715-0: the Hygromycin selection maker of the T-DNA was exchanged with a cassette enabling selection on the basis of CFP-fluorescence in seeds; the egg-cell specific promoter of Cas9 was exchanged with a ubiquitin promoter. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253203v1 for BioRxiv preprint
Proper citation: RRID:Addgene_159747 Copy
Species: Synthetic
Genetic Insert: gRNA scaffold
Vector Backbone Description: Vector Backbone:pCambia; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: Modified from the pHEE401E T-DNA vector described by Wang &al. (2015) Genome Biol 16: 144, doi:10.1186/s13059-015-0715-0: the Hygromycin selection maker of the T-DNA was exchanged with a cassette enabling selection on the basis of YFP-fluorescence in seeds. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253203v1 for BioRxiv preprint
Proper citation: RRID:Addgene_159750 Copy
Species: Synthetic
Genetic Insert: NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21
Vector Backbone Description: Backbone Size:4543; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037199
Comments: Constructs are multipart, modular and generated iteratively.
Proper citation: RRID:Addgene_159959 Copy
Species: Synthetic
Genetic Insert: NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21
Vector Backbone Description: Backbone Size:4543; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037199
Comments: Constructs are multipart, modular and generated iteratively.
Proper citation: RRID:Addgene_159957 Copy
Species: Synthetic
Genetic Insert: mTurquoise2-C1
Vector Backbone Description: Backbone Marker:Addgene plasmid 127862; Backbone Size:4543; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037199
Comments: Constructs are multipart, modular and generated iteratively.
Proper citation: RRID:Addgene_159953 Copy
Species: Synthetic
Genetic Insert: Ypet-3XNLS
Vector Backbone Description: Backbone Size:4543; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037199
Comments: Constructs are multipart, modular and generated iteratively.
Proper citation: RRID:Addgene_159950 Copy
Species: Synthetic
Genetic Insert: mScarlet-optoJNKi
Vector Backbone Description: Backbone Size:4543; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037199
Comments: Constructs are multipart, modular and generated iteratively.
Proper citation: RRID:Addgene_159968 Copy
Species: Synthetic
Genetic Insert: mTurquoise2-optoJNKi-C450A
Vector Backbone Description: Backbone Size:4543; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037199
Comments: Constructs are multipart, modular and generated iteratively.
Proper citation: RRID:Addgene_159969 Copy
Species: Synthetic
Genetic Insert: mTurquoise2-optoJNKi
Vector Backbone Description: Backbone Size:4543; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037199
Comments: Constructs are multipart, modular and generated iteratively.
Proper citation: RRID:Addgene_159966 Copy
Species: Synthetic
Genetic Insert: NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21
Vector Backbone Description: Backbone Size:4651; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037199
Comments: Constructs are multipart, modular and generated iteratively.
Proper citation: RRID:Addgene_159964 Copy
Species: Synthetic
Genetic Insert: NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21
Vector Backbone Description: Backbone Size:4651; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037199
Comments: Constructs are multipart, modular and generated iteratively.
Proper citation: RRID:Addgene_159965 Copy
Species: Synthetic
Genetic Insert: NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21
Vector Backbone Description: Backbone Size:4651; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037199
Comments: Constructs are multipart, modular and generated iteratively.
Proper citation: RRID:Addgene_159963 Copy
Species: Synthetic
Genetic Insert: NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21
Vector Backbone Description: Backbone Size:4543; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037199
Comments: Constructs are multipart, modular and generated iteratively.
Proper citation: RRID:Addgene_159960 Copy
Species: Synthetic
Genetic Insert: NFPnls
Vector Backbone Description: Vector Backbone:pPB-MCSfwd; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33453576
Comments: The Brainbow3.2 cassette was derived from pThy1-Brainbow3.2 (https://www.addgene.org/45179/).
Addgene QC identified several ambiguous bases in the backbone sequence that could not be resolved by standard NGS. These ambiguities are present in the backbone sequence and are not of concern for the function of the construct.
Proper citation: RRID:Addgene_159928 Copy
Species: Synthetic
Genetic Insert: NES-tdTomato-LINuS
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:5335; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31757418
Comments: Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.1038/ncomms5404.
Proper citation: RRID:Addgene_159894 Copy
Species: Synthetic
Genetic Insert: mTurquoise-3XNLS
Vector Backbone Description: Backbone Size:4543; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037199
Comments: Constructs are multipart, modular and generated iteratively.
Proper citation: RRID:Addgene_159949 Copy
Species: Synthetic
Genetic Insert: YPET-3XNLS-optoNES
Vector Backbone Description: Backbone Size:4543; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037199
Comments: Constructs are multipart, modular and generated iteratively.
Proper citation: RRID:Addgene_159947 Copy
Species: Synthetic
Genetic Insert: NLS-Dronpa-optoNES
Vector Backbone Description: Backbone Size:4543; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037199
Comments: Constructs are multipart, modular and generated iteratively.
Proper citation: RRID:Addgene_159944 Copy
Species: Synthetic
Genetic Insert: NLS-YPET-optoNES
Vector Backbone Description: Backbone Size:4543; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037199
Comments: Constructs are multipart, modular and generated iteratively.
Proper citation: RRID:Addgene_159942 Copy
Species: Synthetic
Genetic Insert: NLS-mScarlet-optoNES
Vector Backbone Description: Backbone Size:4543; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037199
Comments: Constructs are multipart, modular and generated iteratively.
Proper citation: RRID:Addgene_159943 Copy
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