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Species: Synthetic
Genetic Insert: 3xERSE-I
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4.29; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32577017
Proper citation: RRID:Addgene_101789 Copy
Species: Homo sapiens
Genetic Insert: SLC25A17 Homology Arms with linker-mEGFP
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human SLC25A17 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: RDPPVAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_101784 Copy
Species: Homo sapiens
Genetic Insert: TUBA1B Homology Arms with mTagRFPT-linker
Vector Backbone Description: Backbone Marker:New England BioLabs; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human TUBA1B in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GGSGGS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_101785 Copy
Vector Backbone Description: Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28886218
Proper citation: RRID:Addgene_101750 Copy
Species: Homo sapiens
Genetic Insert: SRY-box 2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29593326
Proper citation: RRID:Addgene_101761 Copy
Species: Homo sapiens
Genetic Insert: transforming growth factor beta 1 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29593326
Proper citation: RRID:Addgene_101762 Copy
Species: Homo sapiens
Genetic Insert: SMAD2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29593326
Proper citation: RRID:Addgene_101763 Copy
Vector Backbone Description: Backbone Marker:Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html; Backbone Size:4378; Vector Backbone:pCB1532; Vector Types:Fungal expression plasmid, tested in Magnaporthe; Bacterial Resistance:Ampicillin
Comments: Plasmid derived from the pCB1532 vector series Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html
Proper citation: RRID:Addgene_101854 Copy
Vector Backbone Description: Backbone Marker:Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html; Backbone Size:4835; Vector Backbone:pCB1532; Vector Types:Fungal expression plasmid, tested in Magnaporthe; Bacterial Resistance:Ampicillin
Comments: Plasmid derived from the pCB1532 vector series Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html
Proper citation: RRID:Addgene_101855 Copy
Species: Mus musculus
Genetic Insert: Ascl1, Brn2
Vector Backbone Description: Backbone Size:5845; Vector Backbone:pUC19; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28646119
Proper citation: RRID:Addgene_101852 Copy
Species: Mus musculus
Genetic Insert: Evi-1
Vector Backbone Description: Vector Backbone:pLV-EF1a-PGK-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101866 Copy
Species: Mus musculus
Genetic Insert: Evi-1
Vector Backbone Description: Vector Backbone:pLV-EF1a-PGK-BFPT2aPuro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101867 Copy
Species: Mus musculus
Genetic Insert: Mds1-Evi-1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101860 Copy
Species: Homo sapiens
Genetic Insert: Flag-BPTF
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: BPTF insert contains amino acid residues 140-2903 with C1746R, T1752A, D1852N variants compared to the NCBI reference [NM_004459.6].
Proper citation: RRID:Addgene_102242 Copy
Species: Homo sapiens
Genetic Insert: dCas9-HA-BFP-KRAB-NLS
Vector Backbone Description: Backbone Size:9651; Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: Contains HA tag and TagBFP fusion. The plasmid is derived from Addgene Plasmid #46911. The promoter was changed to human EF1a and the NLS was moved to the C-terminus of the fusion protein.
Proper citation: RRID:Addgene_102244 Copy
Species: Homo sapiens
Genetic Insert: CHMP2B
Vector Backbone Description: Backbone Size:6098; Vector Backbone:pTO_LAP_N-DEST; Vector Types:Mammalian Expression, Bacterial Expression, Flp-FRT recombination, Tet-inducible expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28604678
Comments: Part of the plasmid was derived from pmCherry_N_dest from the Helenius Lab (ETH Zuerich)
Proper citation: RRID:Addgene_101848 Copy
Species: Homo sapiens
Genetic Insert: METTL14
Vector Backbone Description: Vector Backbone:pFBOH-LIC notag; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin
Comments: N terminal tag: none. SGC Clone Sample ID: METTL14:JMC097-G06:C232534. SGC or PDG link: http://www.thesgc.org/structures/5TEY/
Proper citation: RRID:Addgene_101891 Copy
Species: Homo sapiens
Genetic Insert: METTL3
Vector Backbone Description: Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 62304); Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: METTL3:JMC094-C09:C231635. SGC or PDG link: http://www.thesgc.org/structures/5TEY/
Proper citation: RRID:Addgene_101892 Copy
Vector Backbone Description: Backbone Marker:Bassik lab (Addgene Plasmid #89359); Vector Backbone:pMCB320; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28474669
Proper citation: RRID:Addgene_101930 Copy
Vector Backbone Description: Backbone Marker:Bassik lab (Addgene Plasmid #89359); Vector Backbone:pMCB320; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28474669
Proper citation: RRID:Addgene_101931 Copy
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