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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pHAGE-ALK
 
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1+ mentions
RRID:Addgene_116712 ALK Homo sapiens Ampicillin PMID:29533785 Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin WT 2026-08-15 01:02:47 1
pPRIME-CMV-Neo-FF3
 
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1+ mentions
RRID:Addgene_11665 FF3 Firefly Chloramphenicol PMID:16141338 CMV promoter transcribes Neo and miR30-based shRNA targeting FF3. The FF3 hairpin can be replaced with any other hairpin by cloning into the EcoR1/Xho1 site. Please note that the full plasmid sequence shown here is for the empty vector only and does not contain the FF3 targeting sequence (AGCTCCCGTGAATTGGAATCCTAGTGAAGCCACAGATGTAGGATTCCAATTCAGCGGGAGCC) Backbone Size:8595; Vector Backbone:pPRIME-CMV-Neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol 2026-08-15 01:02:46 3
pPRIME-TET-GFP-FF3
 
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1+ mentions
RRID:Addgene_11662 FF3 Firefly Chloramphenicol PMID:16141338 TET promoter (minimal CMV promoter containing seven upstream Tet-operator sites) transcribes GFP and miR30-based shRNA targeting FF3. The FF3 hairpin can be replaced with any other hairpin by cloning into the EcoR1/Xho1 site. Please note that the full plasmid sequence shown here is for the empty vector only and does not contain the FF3 targeting sequence (AGCTCCCGTGAATTGGAATCCTAGTGAAGCCACAGATGTAGGATTCCAATTCAGCGGGAGCC) Backbone Size:8226; Vector Backbone:pPRIME-TET-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol 2026-08-15 01:02:46 1
GPI_2xmCherry
 
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1+ mentions
RRID:Addgene_127812 glycosylphosphatidylinositol-mCherry-mCherry Other Kanamycin PMID:30006597 Vector Backbone:mCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:04:40 3
mRuby3-C1
 
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1+ mentions
RRID:Addgene_127808 mRuby3 Other Kanamycin PMID:30006597 Vector Backbone:mEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:04:40 3
pACYC-TtCas6-4xcrRNA4.5
 
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1+ mentions
RRID:Addgene_127764 Synthetic CRISPR array containing four identical T. thermophilus spacers Synthetic Chloramphenicol PMID:31278272 Backbone Marker:Novagen; Backbone Size:3920; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:04:39 4
pSpCas9(BB)-2A-Neo
 
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1+ mentions
RRID:Addgene_127762 Empty backbone Ampicillin pSpCas9(BB)-2A-Puro (PX459) V2.0 was digested with EcoRI to remove the T2A-Puro cassette, and a P2A-Neomycin cassette was amplified by PCR, digested with EcoRI, and ligated. Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene#62988); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:39 3
pUCmini-iCAP-PHP.B4
 
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1+ mentions
RRID:Addgene_127849 Synthetic construct isolate AAV-PHP.B4 VP1 gene Synthetic Ampicillin PMID:32313222 Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction. Backbone Size:6314; Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:04:40 1
MSCV-myc-CAR-2A-Thy1.1
 
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1+ mentions
RRID:Addgene_127890 myc, Mus musculus Ampicillin PMID:30814732 Backbone Marker:Clonetech; Backbone Size:5172; Vector Backbone:MSCV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:04:41 3
FH-TET3-HxDmut-pEF
 
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1+ mentions
RRID:Addgene_127896 TET3-HxDmut Homo sapiens Ampicillin PMID:23563267 Backbone Marker:Invitrogen; Backbone Size:6200; Vector Backbone:PEF1a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:41 1
pN-PITCh-FAID
 
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1+ mentions
RRID:Addgene_127885 GFP-Puro-H3F-AID Homo sapiens Ampicillin PMID:31138654 Vector Backbone:pCRIS-PITCh; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:04:41 1
pHelper_ShCAST
 
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1+ mentions
RRID:Addgene_127922 ShTnsB, ShTnsC, ShTniQ, ShCas12k Ampicillin PMID:31171706 Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:04:41 4
pHelper_ShCAST_sgRNA
 
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1+ mentions
RRID:Addgene_127921 ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites) Ampicillin PMID:31171706 Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:04:41 9
pTarget_CAST
 
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1+ mentions
RRID:Addgene_127926 PSP1 target (GGTT PAM) Chloramphenicol PMID:31171706 Vector Backbone:pACYC; Vector Types:; Bacterial Resistance:Chloramphenicol 2026-08-15 01:04:41 7
pDonor_ShCAST_kanR
 
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10+ mentions
RRID:Addgene_127924 KanR, R6K origin of replication Kanamycin PMID:31171706 Vector Backbone:pUC19, R6K origin of replication; Vector Types:; Bacterial Resistance:Kanamycin 2026-08-15 01:04:41 13
p35S-GW-3Avi
 
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1+ mentions
RRID:Addgene_127918 Chloramphenicol and Ampicillin PMID:33037196 Gateway expression cassette from pEAQ-HT-DEST1 Vector Backbone:pMD19-T; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:04:41 1
CMV-Mito4x-GCaMP6f
 
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1+ mentions
RRID:Addgene_127870 4 repeats of COX8 signal peptide plus GCaMP6f Synthetic Ampicillin PMID:31862210 Backbone Size:5433; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:41 4
pEf1a OsTIR ires NEO pA T2BH
 
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1+ mentions
RRID:Addgene_127910 OsTIR Arabidopsis thaliana Ampicillin PMID:34106828 Vector Backbone:pBSK; Vector Types:Transposon; Bacterial Resistance:Ampicillin 2026-08-15 01:04:41 1
pX330 Human 3' HP1a gRNA
 
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1+ mentions
RRID:Addgene_127907 gRNA for Human 3' HP1a Ampicillin PMID:34106828 Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:04:41 2
Human 3' HP1a AID GFP PuroR
 
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1+ mentions
RRID:Addgene_127906 HP1 Homo sapiens Ampicillin PMID:34106828 Vector Backbone:pBSK; Vector Types:Donor plasmid; Bacterial Resistance:Ampicillin Inserting GFP AID 2A Puro into mouse 3' Hp1a 2026-08-15 01:04:41 2

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