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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pKAM-E-Flag-BFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_101867 | Evi-1 | Mus musculus | Ampicillin | Vector Backbone:pLV-EF1a-PGK-BFPT2aPuro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:22 | 1 | |||
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pcDNA3.1-ME-Flag Resource Report Resource Website 1+ mentions |
RRID:Addgene_101860 | Mds1-Evi-1 | Mus musculus | Ampicillin | Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:22 | 1 | |||
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Adeno FT Resource Report Resource Website 1+ mentions |
RRID:Addgene_101824 | U6_sgRNA(Fgfr3)_U6_sgRNA(Tacc3)_CBh_FLAG-Cas9 | Mus musculus | Ampicillin | PMID:28695888 | Backbone Marker:Gene Transfer Vector Core University of Iowa; Backbone Size:5679; Vector Backbone:pacAd5; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:21 | 1 | ||
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eGFP-proα2(I)-L Resource Report Resource Website |
RRID:Addgene_120160 | Type I procollagen α2 chain | Mus musculus | Ampicillin | PMID:30287488 | These mouse type I procollagen plasmids were designed for immortalized and primary murine osteoblasts and fibroblasts. Their expression in other cells might cause significant disruption of assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in osteoblasts and fibroblasts a fraction of transfected chains, particularly proα2, might be trafficked/degraded as monomers instead of being integrated into procollagen heterotrimers. In our experience, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12 h after transfection. Fluorescent molecules should be incorporated into fibers even when the transfected chains contain the N-propeptide cleavage site, because the cleavage is highly conformation sensitive and always incomplete. We recommend following these guidelines when using the plasmids 1) Only mouse cells that have high expression of endogenous type I procollagen are suitable for studies of physiologically-relevant processes with these constructs. 2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed). 3) Optimization of transfection efficiency is needed for all cell types. 4) Experiments beyond 24h after transfection are not recommended, to avoid excessive accumulation of aggregates and cell malfunction caused by increased procollagen synthesis. 5) Lack of extracellular fluorescent fibers 12-24 h after transfection indicates improper procollagen synthesis/trafficking or cellular malfunction. 6) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death. Contact information: Shakib Omari ([email protected]), Sergey Leikin ([email protected]). | Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Col1a2 exons 2-3 replaced by fluorescent tag | 2026-08-15 01:03:21 | 0 |
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mCh-KIF5A*-strep Resource Report Resource Website 1+ mentions |
RRID:Addgene_120163 | kinesin family member 5A | Mus musculus | Kanamycin | PMID:31100061 | Backbone Marker:Clontech; Backbone Size:4727; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Truncated KIF5A: 1-559 aa | 2026-08-15 01:03:21 | 1 | |
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mCh-KIF1A*-strep Resource Report Resource Website 1+ mentions |
RRID:Addgene_120165 | kinesin family member 1A | Mus musculus | Kanamycin | PMID:31100061 | Backbone Marker:Clontech; Backbone Size:4727; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Truncated KIF1A: 1-405 aa | 2026-08-15 01:03:19 | 1 | |
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mCh-BICD2*-strep Resource Report Resource Website 1+ mentions |
RRID:Addgene_120168 | BICD cargo adaptor 2 | Mus musculus | Kanamycin | PMID:31100061 | Backbone Marker:Clontech; Backbone Size:4713; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Truncated BICD2: 15-595 aa | 2026-08-15 01:03:19 | 1 | |
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pLV-EF1a-V5-Vrk2 Resource Report Resource Website |
RRID:Addgene_120240 | Vrk2 | Mus musculus | Ampicillin | PMID:31142202 | Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:03:22 | 0 | ||
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pLV-EF1a-V5-Sec61B Resource Report Resource Website |
RRID:Addgene_120244 | Sec61B | Mus musculus | Ampicillin | PMID:31142202 | Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:03:20 | 0 | ||
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pLV-EF1a-V5-Lem2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_120245 | Lem2 | Mus musculus | Ampicillin | PMID:31142202 | Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:03:20 | 1 | ||
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pLV-EF1a-V5-Mfsd10 Resource Report Resource Website |
RRID:Addgene_120238 | Mfsd10 | Mus musculus | Ampicillin | PMID:31142202 | Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:03:22 | 0 | ||
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pLV-EF1a-V5-Tmem128 Resource Report Resource Website |
RRID:Addgene_120239 | Tmem128 | Mus musculus | Ampicillin | Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:03:20 | 0 | |||
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pLV-EF1a-V5-Arl6ip6 Resource Report Resource Website |
RRID:Addgene_120235 | Arl6ip6 | Mus musculus | Ampicillin | PMID:31142202 | Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:03:22 | 0 | ||
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pLV-EF1a-V5-Smpd4 Resource Report Resource Website |
RRID:Addgene_120237 | Smpd4 | Mus musculus | Ampicillin | PMID:31142202 | Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:03:20 | 0 | ||
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jk96 Resource Report Resource Website |
RRID:Addgene_120177 | CAG:mCherry:P2A:HA_NLGN | Mus musculus | Ampicillin | Vector Backbone:paavCAG-Jx; Vector Types:AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:03:21 | 0 | |||
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pRK5 FLAG membrane-bound lipin-1 (TMD) Resource Report Resource Website |
RRID:Addgene_120277 | Lipin-1 | Mus musculus | Ampicillin | PMID:30718857 | Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:03:20 | 0 | ||
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pRK5 FLAG membrane-bound lipin-1 (delta200) Resource Report Resource Website |
RRID:Addgene_120278 | Lipin-1 | Mus musculus | Ampicillin | PMID:30718857 | Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:03:22 | 0 | ||
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PB-TAC-OKMS-Cas9 Resource Report Resource Website |
RRID:Addgene_120353 | OKMS cassette | Mus musculus | Ampicillin | PMID:30639212 | Please Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to depositor's provided sequence for this section of the plasmid. | Vector Backbone:PB-TAC-Cas9; Vector Types:Mammalian Expression, CRISPR, piggyBac transposon; Bacterial Resistance:Ampicillin | Klf4 [10-483] | 2026-08-15 01:03:23 | 0 |
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PB-TAC-OK+9MS-Cas9 Resource Report Resource Website |
RRID:Addgene_120354 | OK+9MS cassette | Mus musculus | Ampicillin | PMID:30639212 | Vector Backbone:PB-TAC-Cas9; Vector Types:Mammalian Expression, CRISPR, piggyBac transposon; Bacterial Resistance:Ampicillin | Klf4 [1-483] | 2026-08-15 01:03:21 | 0 | |
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pLenti-CMV-GFP-Sox11 Resource Report Resource Website 1+ mentions |
RRID:Addgene_120387 | sox11 | Mus musculus | Ampicillin | PMID:30401432 | Backbone Size:7424; Vector Backbone:pLenti CMV GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:03:22 | 1 |
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