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Species: Synthetic
Genetic Insert: 3xFLAG
Vector Backbone Description: Backbone Size:4578; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26861996
Proper citation: RRID:Addgene_127862 Copy
Species: Discosoma
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:4638; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26861996
Proper citation: RRID:Addgene_127861 Copy
Species: Mus musculus
Genetic Insert: Nos1
Vector Backbone Description: Backbone Size:4641; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26861996
Proper citation: RRID:Addgene_127869 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.N VP1 gene
Vector Backbone Description: Backbone Size:6314; Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32313222
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_127851 Copy
Species: E. coli
Genetic Insert: bla
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31604953
Comments: This plasmid is a derivative of repressilator plasmids presented in: Potvin-Trottier et al, 2016, Nature.
Proper citation: RRID:Addgene_127855 Copy
Genetic Insert: traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites
Vector Backbone Description: Vector Backbone:pBS; Vector Types:Drosophila OSC reporter construct; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31384064
Proper citation: RRID:Addgene_128010 Copy
Species: Drosophila melanogaster
Genetic Insert: Gal4-DBD-3xFlag
Vector Backbone Description: Vector Backbone:modified from pAcM; Vector Types:Gal4 entry vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31384064
Proper citation: RRID:Addgene_128014 Copy
Species: Synthetic
Genetic Insert: iGlucoSnFR-TS
Vector Backbone Description: Vector Backbone:pAAV.CAG...WPRE.SV40pA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31106909
Comments: Commercial use is protected by US Patent 9939437
Proper citation: RRID:Addgene_127952 Copy
Vector Backbone Description: Vector Backbone:pLEX Mcs; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128045 Copy
Vector Backbone Description: Vector Backbone:pcdna3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128047 Copy
Species: Synthetic
Genetic Insert: Cpf1
Vector Backbone Description: Backbone Size:10152; Vector Backbone:pAC575; Vector Types:Bacterial Expression, CRISPR, Fungal Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31061713
Comments: AMA1 sequence contains multiple ambiguous bases that are likely mixed nucleotide populations. AMA1 is a palindrome and thus difficult to sequence. Dep. confirms the mixed population does not affect plasmid function.
Proper citation: RRID:Addgene_127984 Copy
Vector Backbone Description: Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128038 Copy
Species: Synthetic
Genetic Insert: Cpf1
Vector Backbone Description: Backbone Size:10868; Vector Backbone:pAC573; Vector Types:Bacterial Expression, CRISPR, Fungal Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31061713
Proper citation: RRID:Addgene_127982 Copy
Vector Backbone Description: Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128037 Copy
Species: Synthetic
Genetic Insert: Cpf1
Vector Backbone Description: Backbone Size:10239; Vector Backbone:pAC572; Vector Types:Bacterial Expression, CRISPR, Fungal Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31061713
Comments: AMA1 sequence contains multiple ambiguous bases that are likely mixed nucleotide populations. AMA1 is a palindrome and thus difficult to sequence. Dep. confirms the mixed population does not affect plasmid function.
Proper citation: RRID:Addgene_127981 Copy
Species: Aspergillus fumigatus
Genetic Insert: pyrG
Vector Backbone Description: Vector Backbone:custom; Vector Types:Bacterial Expression, Fungal Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31061713
Comments: AMA1 is a palindrome which causes issues sequencing. There are a few nucleotides with a mixed population. This however does not affect plasmid function.
Published in: Nødvig CS, Hoof JB, Kogle ME, Jarczynska ZD, Lehmbeck J, Klitgaard DK, et al. Efficient oligo nucleotide mediated CRISPR-Cas9 gene editing in Aspergilli. Fungal Genet Biol. 2018;115:78–89, https://doi.org/10.1016/j.fgb.2018.01.004.
Proper citation: RRID:Addgene_127973 Copy
Species: Streptoalloteichus hindustanus
Genetic Insert: ble (bleomycin resistance marker)
Vector Backbone Description: Vector Backbone:custom; Vector Types:Bacterial Expression, Fungal Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31061713
Comments: AMA1 sequence contains multiple ambiguous bases that are likely mixed nucleotide populations. AMA1 is a palindrome and thus difficult to sequence. Dep. confirms the mixed population does not affect plasmid function.
Proper citation: RRID:Addgene_127976 Copy
Species: Homo sapiens
Genetic Insert: Hypoxia response element (x3) from PGK1 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pGL4.22; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29861175
Proper citation: RRID:Addgene_128095 Copy
Genetic Insert: sgRNA AAVS1 + GFP
Vector Backbone Description: Vector Backbone:AAV; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32076642
Proper citation: RRID:Addgene_128119 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments:
Proper citation: RRID:Addgene_128073 Copy
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