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Species: Other
Genetic Insert: NLS-AsCas12f1-NLS
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34475565
Proper citation: RRID:Addgene_171613 Copy
Species: Other
Genetic Insert: AsCas12f1_sgRNA_1
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34475565
Proper citation: RRID:Addgene_171611 Copy
Species: Other
Genetic Insert: sgRNA scaffold-nSaCas9-NLS
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33972728
Proper citation: RRID:Addgene_171695 Copy
Species: Other
Genetic Insert: MBP-mCherry-8xHis-SacB(-)
Vector Backbone Description: Backbone Marker:Center for Eukaryotic Structural Genomics (CESG); Vector Backbone:pVP65K; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34749604
Comments: Korean patent: Auto-induction Plasmid, 10-2286426.
For more information, please see Chae YK (2021) FASEB J, https://doi.org/10.1096/fasebj.2021.35.S1.01779
Proper citation: RRID:Addgene_171646 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34270613
Proper citation: RRID:Addgene_171599 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression, Synthetic Biology, Promoterless Bxb1 DNA recombination; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34270613
Proper citation: RRID:Addgene_171598 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression, Synthetic Biology, Promoterless Bxb1 DNA recombination; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34270613
Proper citation: RRID:Addgene_171597 Copy
Species: Other
Genetic Insert: YFP
Vector Backbone Description: Vector Backbone:pKS; Vector Types:Toxoplasma gondii; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19218426
Proper citation: RRID:Addgene_83128 Copy
Species: Other
Genetic Insert: shGFP
Vector Backbone Description: Backbone Marker:Addgene #21915; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161592
Comments: The targeting sequence was identified from the Addgene #30323. Used as control knockdown vector.
Proper citation: RRID:Addgene_83085 Copy
Species: Other
Genetic Insert: shLuc
Vector Backbone Description: Backbone Marker:Addgene #21915; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161592
Comments: The targeting sequence was identified from the TRC (TRCN0000072243). Used as control knockdown vector.
Proper citation: RRID:Addgene_83092 Copy
Species: Other
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:Addgene #25890; Backbone Size:9377; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161592
Comments: Plasmid for stable expression of LacZ with C-terminal V5 tag.
Proper citation: RRID:Addgene_83099 Copy
Species: Other
Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25853840
Comments: Precursor strain: ElectroMAX DH10B E. coli cells
Landing pad cassette at at the aptI/gidB locus includes in this order: I-SceI restriction site - Landing Pad 1 (LP1) sequence - tetA gene - Landing Pad 2 (LP2) sequence - I-SceI restriction site.
This strain was made based on the technology described in the Kulman and Cox 2010 Nucleic Acids Res. paper: Site-specific chromosomal integration of large synthetic constructs. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847246/
Proper citation: RRID:Addgene_83036 Copy
Species: Other
Genetic Insert: shRNA to Luciferase
Vector Backbone Description: Backbone Marker:OD260 Inc; Vector Backbone:pAd388; Vector Types:Retroviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28969075
Proper citation: RRID:Addgene_83275 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pKS; Vector Types:Toxoplasma gondii; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19218426
Proper citation: RRID:Addgene_83364 Copy
Species: Other
Genetic Insert: NatMx3
Vector Backbone Description: Vector Backbone:pRSII426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid is similar to pML104 except that the URA3 marker has been switched for NatMx3 marker using Gibson Assembly. Cloning of guide sequence using BclI-SwaII restriction sites requires growth in dam- E. coli cells.
Proper citation: RRID:Addgene_83477 Copy
Species: Other
Genetic Insert: KanMX4
Vector Backbone Description: Vector Backbone:pRSII426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid is similar to pML104 except that the URA3 marker has been switched for KanMX4 marker using Gibson Assembly. Cloning of guide sequence using BclI-SwaII restriction sites requires growth in dam- E. coli cells.
Proper citation: RRID:Addgene_83476 Copy
Species: Other
Genetic Insert: WingGFP-tan
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM T-easy; Vector Types:Bacterial Expression, Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27494619
Comments: To be used as a selectable marker in two-stage CRISPR-mediated allele replacement strategies as described in Lamb, et al 2016. This donor contains a selectable green fluorescent protein driven by an enhancer from the D. melanogaster yellow gene that can be screened in the developing pupal wing. The current insert contains homology arms targeting the D. americana tan gene, which can be replaced with homology arms targeting the experimenter's locus of choice.
Construct contains:
5’ homology arm
5’ target PAM
Unique portion of 5’ target site for reporter excision (restriction sites: BglII, BsiWI, Acc65I)
Pupal wing enhancer from D. melanogaster yellow gene
EGFP with Hsp70 promoter and SV40 polyadenylation signal
Unique portion of 3’ target site for reporter excision (restriction sites: NarI, Bsu36I, ClaI)
3’ homology arm
3’ target PAM
Restriction sites within pGEM backbone for homology arm removal: 5’ end – ApaI, SphI; 3’ end – SbfI, MluI
Proper citation: RRID:Addgene_83473 Copy
Species: Other
Genetic Insert: HphMX4
Vector Backbone Description: Vector Backbone:pRSII426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid is similar to pML104 except that the URA3 marker has been switched for hphMX4 marker using Gibson Assembly. Cloning of guide sequence using BclI-SwaII restriction sites requires growth in dam- E. coli cells.
Proper citation: RRID:Addgene_83475 Copy
Species: Other
Genetic Insert: 3XP3.RFP-tan
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM T-easy; Vector Types:Bacterial Expression, Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27494619
Comments: To be used as a selectable marker in two-stage CRISPR-mediated allele replacement strategies as described in Lamb, et al 2016. This donor contains a selectable red fluorescent protein maker that can be screened in the adult eye. The current insert contains homology arms targeting the D. americana tan gene, which can be replaced with homology arms targeting the experimenter's locus of choice.
Construct contains:
5’ homology arm
5’ target PAM
Unique portion of 5’ target site for reporter excision (restriction sites: BglII, BsiWI, Acc65I)
3XP3 eye-expression Promoter
RFP with α-tubulin 3’UTR
Unique portion of 3’ target site for reporter excision (restriction sites: NarI, Bsu36I, ClaI)
3’ homology arm
3’ target PAM
Restriction sites for homology arm removal: 5’ end – ApaI, NcoI; 3’ end – SexAI, MluI
Proper citation: RRID:Addgene_83474 Copy
Species: Other
Genetic Insert: YFP-CFP
Vector Backbone Description: Backbone Marker:Muller Lab; Vector Backbone:based on pDH3 and pDH5; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12073355
Comments: For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
Proper citation: RRID:Addgene_83803 Copy
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