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Species: Saccharomyces cerevisiae
Genetic Insert: SAD1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24681967
Comments: Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus.
Proper citation: RRID:Addgene_90307 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SAD1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24681967
Comments: Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus.
Proper citation: RRID:Addgene_90306 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SAD1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24681967
Comments: Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus.
Proper citation: RRID:Addgene_90302 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP8
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Vector Backbone:pDEST15; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27072132
Comments: Ledoux and Guthrie, JBC, 2016
Proper citation: RRID:Addgene_90264 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BRR2
Vector Backbone Description: Backbone Size:4957; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27072132
Comments: Ledoux and Guthrie, JBC, 2017
Proper citation: RRID:Addgene_90263 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BRR2
Vector Backbone Description: Backbone Size:4957; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27072132
Comments: Ledoux and Guthrie, JBC, 2017
Proper citation: RRID:Addgene_90260 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BRR2
Vector Backbone Description: Backbone Size:4957; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27072132
Comments: Ledoux and Guthrie, JBC, 2017
Proper citation: RRID:Addgene_90261 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUB2
Vector Backbone Description: Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11156604
Comments: Addgene NGS results found E16G, H60Y, A114T, K202E, E222G, and E267G in the SUB2 translation compared to NP_010199.1
Proper citation: RRID:Addgene_90455 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:3882; Vector Backbone:2 micron; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: For more information see https://benchling.com/pub/ellis-crispr-tools
Proper citation: RRID:Addgene_90519 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Yeast sgRNA expression vector
Vector Backbone Description: Backbone Size:1753; Vector Backbone:N/A; Vector Types:Yeast Expression, CRISPR, Yeast gap repair; Bacterial Resistance:Ampicillin
Comments: Refer to https://benchling.com/pub/ellis-crispr-tools for guidelines on how to use
Proper citation: RRID:Addgene_90516 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP8
Vector Backbone Description: Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28416677
Proper citation: RRID:Addgene_90976 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP8
Vector Backbone Description: Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28416677
Proper citation: RRID:Addgene_90975 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP8
Vector Backbone Description: Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28416677
Proper citation: RRID:Addgene_90977 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP8
Vector Backbone Description: Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28416677
Proper citation: RRID:Addgene_90974 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP8
Vector Backbone Description: Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28416677
Proper citation: RRID:Addgene_90970 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP8
Vector Backbone Description: Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28416677
Proper citation: RRID:Addgene_90969 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP8
Vector Backbone Description: Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28416677
Proper citation: RRID:Addgene_90968 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP8
Vector Backbone Description: Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28416677
Proper citation: RRID:Addgene_90967 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:4163; Vector Backbone:2 micron; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: For more information see https://benchling.com/pub/ellis-crispr-tools
Proper citation: RRID:Addgene_90960 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:3728; Vector Backbone:2 micron; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: For more information see https://benchling.com/pub/ellis-crispr-tools
Proper citation: RRID:Addgene_90963 Copy
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